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日用化学工业（中英文） ›› 2025, Vol. 55 ›› Issue (5): 581-590.doi: 10.3969/j.issn.2097-2806.2025.05.006

• 开发与应用 • 上一篇    下一篇

白花蛇舌草提取液对日光性皮炎小鼠的保护作用及机制研究

马悦文1,2,3,李小静4,黄雪锋4,*(),陈贤5,王春丽1,2,3,*()   

  1. 1.华东理工大学 药学院，上海 200237
    2.制药工程与过程化学教育部工程研究中心，上海 200237
    3.上海市新药设计重点实验室，上海 200237
    4.珀莱雅化妆品股份有限公司，浙江 杭州 310012
    5.广州伽能生物科技有限公司，广东 广州 511335
  • 收稿日期:2024-12-17 修回日期:2025-04-11 出版日期:2025-05-22 发布日期:2025-06-11
  • 基金资助:
    国家科技重大专项(2018ZX09735002)

Protective effect and mechanism of Hedyotis diffusa extract on mice with solar dermatitis

Yuewen Ma1,2,3,Xiaojing Li4,Xuefeng Huang4,*(),Xian Chen5,Chunli Wang1,2,3,*()   

  1. 1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
    2. Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, Shanghai 200237, China
    3. Shanghai Key Laboratory of New Drug Design, Shanghai 200237, China
    4. PROYA Cosmetics Co., Ltd., Hangzhou, Zhejiang 310012, China
    5. Eneplus Biotech Co., Ltd., Guangzhou, Guangdong 511335, China
  • Received:2024-12-17 Revised:2025-04-11 Online:2025-05-22 Published:2025-06-11
  • Contact: *E-mail: wangchunli@ecust.edu.cn（Chunli Wang）; huangxuefeng70@proya.com（Xuefeng Huang）.

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摘要：

研究白花蛇舌草提取液对紫外线B（UVB）诱导的日光性皮炎小鼠的抗氧化和抗炎作用。使用KM小鼠进行UVB照射7天，同时外涂质量分数1%和2%的白花蛇舌草提取液进行治疗。对小鼠皮肤组织进行苏木精-伊红染色（HE）染色、苦味酸-酸性品红法（VG）染色观察小鼠皮肤状态。通过生化和免疫组化检测皮肤组织中氧化应激指标NOS、NO、T-SOD以及炎症指标TNF-α、IL-6、IL-1β水平，通过qRT-PCR和Western blotting检测皮肤组织中Nrf2、Keap1、HO-1、NQO1、GCLC、NF-κB、HMGB1、p65的mRNA或蛋白表达水平。结果表明，与UVB组对比，白花蛇舌草提取液1%组和2%组小鼠皮肤损伤程度有所改善，胶原纤维排列整齐，胶原密度升高，NOS、NO水平降低、T-SOD水平提高，TNF-α、IL-6、IL-1β表达水平降低，皮肤组织中Nrf2、Keap1、HO-1、NQO1、GCLC的mRNA表达水平升高，NF-κB、HMGB1 mRNA表达水平降低，皮肤组织中Nrf2、Keap1、HO-1、NQO1、GCLC、p65的蛋白相对表达量升高，HMGB1的蛋白表达水平降低。可见，白花蛇舌草提取液可以改善UVB介导的小鼠日光性皮炎，其抗氧化抗炎机制可能与Nrf2和NF-κB信号通路有关。

关键词: 白花蛇舌草提取液, 紫外线B, 日光性皮炎, 抗氧化, 抗炎

Abstract:

The paper aimed to study the antioxidant and anti-inflammatory effects of Hedyotis diffusa extract on mice with ultraviolet B （UVB）-induced solar dermatitis. KM mice were subjected to UVB irradiation for 7 days, which were also treated with topical application of 1% and 2% Hedyotis diffusa extract. Hematoxylin-eosin （HE） staining, and picric acid-acidic magenta method （VG） staining were performed to observe the state of mouse skin. The levels of oxidative stress indicators NOS, NO, T-SOD, and inflammation indicators TNF-α, IL-6, and IL-1β were detected by biochemistry and immunohistochemistry, and the mRNA or protein levels of Nrf2, Keap1, HO-1, NQO1, GCLC, NF-κB, HMGB1 and p65 were detected by qRT-PCR and western blotting in skin tissues. Compared with the UVB group, the degree of skin damage in mice in the 1% and 2% Hedyotis diffusa extract groups is improved, with collagen fibers aligned, collagen density increased, NOS and NO levels decreased, T-SOD levels increased, TNF-α, IL-6, IL-1β expression levels decreased, mRNA expression levels of Nrf2, Keap1, HO-1, NQO1, and GCLC in skin tissue increased, NF-κB, HMGB1 mRNA expression levels decreased, the relative protein expression levels of Nrf2, Keap1, HO-1, NQO1, GCLC, and p65 in skin tissues increased, and the protein expression level of HMGB1 decreased. It can be seen that Hedyotis diffusa extract can ameliorate UVB-mediated solar dermatitis in mice, and its antioxidant and anti-inflammatory mechanism may be related to Nrf2 and NF-κB signaling pathway.

Key words: Hedyotis diffusa extract, ultraviolet B, solar dermatitis, antioxidant, anti-inflammatory

中图分类号: 

  • TQ658