羟基积雪草苷通过激活Nrf2-HO-1通路发挥抗衰老及皮肤修复作用

doi:10.3969/j.issn.2097-2806.2024.06.009

摘要/Abstract

摘要：

考察羟基积雪草苷（MA）对D-半乳糖诱导的皮肤衰老小鼠的抗衰老及皮肤修复作用。将小鼠分为6组（n=12）：正常组（N）、模型组（M）、低、中、高剂量MA组（L-MA，M-MA，H-MA）和高剂量MA+Nrf2抑制剂ML385组（H-MA+ML385）。分别检测了各组小鼠的体重、皮肤含水量、皮肤组织中的抗氧化指标（SOD、CAT、GSH-Px和MDA）、HYP和HA水平。采用HE染色评价了小鼠皮肤组织结构。通过RT-qPCR检测了小鼠皮肤组织中MMP-1、MMP-3、Nrf2、Keap1和HO-1的转录水平。通过Western blotting检测了小鼠皮肤组织中Nrf2、Keap1和HO-1的蛋白表达水平。结果显示，与M组比较，L-MA组、M-MA组和H-MA组小鼠皮肤含水量升高，表皮和真皮厚度增加，皮肤结构明显改善，SOD、CAT和GSH-Px水平升高，MDA水平降低，HYP和HA水平升高，MMP-1和MMP-3 mRNA相对表达量降低，Nrf2和HO-1的mRNA和蛋白相对表达量升高，Keap1 mRNA和蛋白相对表达量降低（P<0.05）。ML385部分减弱了MA对Nrf2-HO-1通路的激活效果，并减弱了MA的抗衰老和皮肤修复作用（P<0.05）。本研究表明，MA具有良好的抗衰老和皮肤修复作用，其机制与激活Nrf2-HO-1通路有关。

关键词: 羟基积雪草苷, 抗衰老, 皮肤修复, 抗氧化, Nrf2-HO-1通路

Abstract:

The study aimed to investigate the anti-aging and skin repair effects of madecassoside （MA） on D-galactose-induced skin aging mice. Mice were divided into 6 groups （n=12）: normal group （N）, model group （M）, low, medium, high-dose MA groups （L-MA, M-MA, H-MA） and high-dose MA+Nrf2 inhibitor ML385 group （H-MA+ML385）. The body weight, skin water content, antioxidant indicators （SOD, CAT, GSH-Px and MDA）, HYP and HA levels in skin tissue of mice in each group were detected. Skin histology was evaluated by HE staining. The transcript levels of MMP-1, MMP-3, Nrf2, Keap1 and HO-1 in skin tissues were detected by RT-qPCR. The protein expression levels of Nrf2, Keap1 and HO-1 in skin tissue were detected by Western blotting. The results show that compared with the M group, the skin water content of mice in L-MA group, M-MA group and H-MA group increases, the thickness of the epidermis and dermis increases, the skin structure is significantly improved, the levels of SOD, CAT and GSH-Px increase, the level of MDA decreases, the level of HYP and HA increases, the relative expression of MMP-1 and MMP-3 mRNA decreases, the relative expression of Nrf2 and HO-1 mRNA and protein increases, and the relative expression of Keap1 mRNA and protein decrease （P<0.05）. ML385 partially weakens the activation effect of MA on the Nrf2-HO-1 pathway, and weakens the anti-aging and skin repair effects of MA （P<0.05）. This study shows that MA has good anti-aging and skin repair effects, and its mechanism is related to the activation of Nrf2-HO-1 pathway.

Key words: madecassoside, anti-aging, skin repair, antioxidant, Nrf2-HO-1 pathway

中图分类号:

TQ658

王丹, 俞舜, 王玉英, 李京玲, 刘晨阳, 吕国忠. 羟基积雪草苷通过激活Nrf2-HO-1通路发挥抗衰老及皮肤修复作用[J]. 日用化学工业（中英文）, 2024, 54(6): 683-690.

Dan Wang, Shun Yu, Yuying Wang, Jingling Li, Chenyang Liu, Guozhong Lv. Madecassoside exerts anti-aging and skin repair effects by activating Nrf2-HO-1 pathway[J]. China Surfactant Detergent & Cosmetics, 2024, 54(6): 683-690.

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Gene	Sequence
MMP-1	F: 5'-GATGGATCCCAAGGCATATATGGAGGTTCC-3'
MMP-1	R: 5'-TTGGAATTCCGGACTTCATCTCTGTCGG-3'
MMP-3	F: 5'-TATGGATCCCCCCCTGACTCCCCTGAG-3'
MMP-3	R: 5'-ATGGAATTCAAGCTTCCTGAGGT-3'
Nrf2	F: 5'-GAGACGGCCATGACTGAT-3'
Nrf2	R: 5'-TGTGGAACATCTGGTAGACGGC-3'
Keap1	F: 5'-TGCCCCTGTGGTCAAAGTG-3'
Keap1	R: 5'-GGTTCGGTTACCGTCCTGC-3'
HO-1	F: 5'-CACGCATATACCCGCTACCT-3'
HO-1	R: 5'-CCAGAGTGTTCATTCGAGCA-3'
GAPDH	F: 5'-GTGAAGGTCGGAGTCAACG-3'
GAPDH	R: 5'-TGAGGTCAATGAAGGGGTC-3'