4-丁基间苯二酚微乳凝胶制备及美白抗氧化研究

doi:10.3969/j.issn.2097-2806.2024.05.004

摘要/Abstract

摘要：

通过配方筛选优化制备含有4-丁基间苯二酚的肉桂油微乳凝胶，考察其制剂学性质，并对其进行美白及抗氧化性能的评价。含有4-丁基间苯二酚的肉桂油微乳外观澄清透明，粒径15.68 nm、多分散系数（PDI）0.185。以卡波姆和白芨多糖作为凝胶基质制备微乳凝胶，该微乳凝胶的pH值为6.0，黏度为74.8 Pa·s，符合外用凝胶剂质量要求。通过酪氨酸酶抑制率实验测定4-丁基间苯二酚肉桂油微乳凝胶的美白功效，该微乳凝胶的酪氨酸酶抑制率随着质量浓度增加而逐渐增强，且微乳凝胶的酪氨酸酶抑制率均高于同等质量浓度下的游离4-丁基间苯二酚和熊果苷，表明4-丁基间苯二酚肉桂油微乳凝胶具有良好的美白功效。通过4-丁基间苯二酚肉桂油微乳凝胶对过氧化氢（H₂O₂）诱导B16-F10细胞氧化损伤保护作用的测定，评价其抗氧化性能，结果表明4-丁基间苯二酚肉桂油微乳凝胶在0.1 μg/mL质量浓度下无细胞毒性，通过抑制ROS产生抑制H₂O₂引起的B16-F10细胞损伤。

关键词: 4-丁基间苯二酚, 肉桂油, 微乳凝胶, 美白, 抗氧化

Abstract:

The 4-butylresorcinol-containing cinnamon oil microemulsion-gel was prepared and its pharmaceutical properties were investigated. Its whitening and antioxidant properties were evaluated. Cinnamon oil microemulsion containing 4-butylresorcinol is clear and transparent in appearance. Its particle size is 15.68 nm and the coefficient of dispersity （PDI） is 0.185. The microemulsion-gel is prepared by using carbopol and Bletilla striata （T.） Reichb. F. polysaccharides as the gel matrix with the pH value at 6.0 and the viscosity at 74.8 Pa·s. In this study, the whitening effect of 4-butylresorcinol-containing cinnamon oil microemulsion-gel was determined by measuring the tyrosinase inhibition. It is shown that the tyrosinase inhibition of 4-butylresorcinol-loaded cinnamon oil microemulsion-gel gradually enhances with its concentration increasing. The tyrosinase inhibition activity of microemulsion-gels is higher than that of the free 4-butylresorcinol and arbutin at the same concentration. The antioxidant effect of 4-butylresorcinol-loaded cinnamon oil microemulsion-gel on hydrogen peroxide （H₂O₂） -induced oxidative damage was tested in B16-F10 cells. The results show that microemulsion-gel has excellent antioxidant effect by reducing ROS production.

Key words: 4-butylresorcinol, cinnamon oil, microemulsion-gel, whitening, antioxidation

中图分类号:

TQ658

魏楚原, 张晓萍, 潘敬, 彭佩, 张雅鹃, 穆朝峰. 4-丁基间苯二酚微乳凝胶制备及美白抗氧化研究[J]. 日用化学工业（中英文）, 2024, 54(5): 520-526.

Chuyuan Wei, Xiaoping Zhang, Jing Pan, Pei Peng, Yajuan Zhang, Chaofeng Mu. Preparation of 4-butylresorcinol-loaded cinnamon oil microemulsion-gel and its whitening and antioxidative effects[J]. China Surfactant Detergent & Cosmetics, 2024, 54(5): 520-526.

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处方	V（油相） ∶V（乳化剂/助乳化剂） ∶V（水相）	外观	离心后	粒径/nm	PDI
①	0.5∶1.5∶4.5	透明，淡蓝色乳光	透明，无沉淀	16.02±0.16	0.103±0.02
②	0.5∶1∶5	近透明，略显淡蓝色乳光	沉淀	204.63±4.77	0.371±0.03
③	1∶1.5∶5	近透明，略显淡蓝色乳光	沉淀	126.00±5.60	0.565±0.03

参数	N₁	N₂	N₃	平均值±SD
粒径/nm	15.64	15.70	15.71	15.68±0.03
PDI	0.193	0.184	0.179	0.185±0.006
Zeta电位/mV	+0.027 1	-0.025 9	-0.032 0	-0.01±0.003