酶水解法制备黄芪素及应用研究

doi:10.3969/j.issn.2097-2806.2023.05.008

摘要/Abstract

摘要：

探讨酶水解法制备黄芪素及其应用研究。以黄芪素的提取率为评价指标，采用酶水解法提取黄芪素，使用高效液相色谱仪（HPLC）进行测定。通过构建衰老模型小鼠，将酶水解法制备的黄芪素作用于衰老模型小鼠，观察衰老模型小鼠学习记忆能力及脑组织氧化水平检测。结果显示，酶水解法正交试验较优条件为：当料液比1∶10，提取次数3次，提取温度80 ℃，提取时间1 min时，黄芪素提取率最高。与对照组相比，黄芪素作用衰老模型小鼠后，能够显著改善其学习记忆能力（P<0.05）；经黄芪素处理后，衰老模型小鼠脑组织中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）含量显著增加（P<0.05），而丙二醛（MDA）含量显著下降（P<0.05）。本研究中，通过优化黄芪素制备方法，并检测了黄芪素抗衰老能力，该研究为将来黄芪素的进一步开发和临床应用奠定了理论和实践基础。

关键词: 酶水解法, 黄芪素, 衰老模型小鼠, 抗氧化, 应用研究

Abstract:

This research investigated the preparation of astragalin by enzymatic hydrolysis method and its application. Using the extraction rate of Astragalus as the evaluation index, astragalin was extracted by enzymatic hydrolysis method and determined by using high performance liquid chromatography （HPLC）. By constructing aging model mice, the astragalin prepared by enzymatic hydrolysis method was applied to the aging model mice, and the learning and memory ability and the oxidation level of brain tissue of the aging model mice were observed. The results show that the orthogonal test of enzymatic hydrolysis method is superior when the material-liquid ratio is 1∶10, the number of extractions is 3 times, the extraction temperature is 80 ℃ and the extraction time is 1 min, with the highest extraction rate of Astragalus. Compared with the control group, astragalin significantly improves the learning and memory abilities of the aging model mice （P<0.05）. The contents of superoxide dismutase （SOD） and catalase （CAT） in the brain tissues of the aging model mice increase significantly after treatment with astragalin （P<0.05）, while the contents of malondialdehyde （MDA） decrease significantly （P<0.05）. In this study, by optimising the preparation method of Astragalus and testing the anti-ageing ability of Astragalus, the study lays the theoretical and practical foundation for the further development and clinical application of Astragalus in the future.

Key words: enzymatic hydrolysis, Astragalus, aging model mice, anti-oxidation, application research

中图分类号:

TQ658

亓国锋, 李刚刚, 安晏. 酶水解法制备黄芪素及应用研究[J]. 日用化学工业（中英文）, 2023, 53(5): 544-550.

Qi Guofeng, Li Ganggang, An Yan. Study on the preparation of Astragalus by enzymatic hydrolysis and its application[J]. China Surfactant Detergent & Cosmetics, 2023, 53(5): 544-550.

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水平	A 提取温度/℃	B 提取时间/min	C 料液比/（g∶mL）	D 提取次数/次
1	60	1	1∶12	1
2	70	3	1∶10	2
3	80	5	1∶ 8	3

组号	D	C	B	A	黄芪素含量/（mg/g）
1	1	1	1	1	1.590 7
2	1	2	2	2	1.629 3
3	1	3	3	3	1.436 0
4	2	1	2	3	2.117 5
5	2	2	3	1	2.279 7
6	2	3	1	2	2.302 4
7	3	1	3	2	2.890 6
8	3	2	1	3	2.940 1
9	3	3	2	1	2.912 4
均值1	1.498	2.103	2.146	2.153
均值2	2.187	2.170	2.118	2.137
均值3	2.806	2.102	2.193	2.161
极差R	1.243	0.076	0.035	0.063