莲子心总黄酮多元醇提取工艺筛选及其性能研究

doi:10.3969/j.issn.1001-1803.2023.04.005

摘要/Abstract

摘要：

莲子心作为一种药食同源的植物，极具开发潜力。该研究通过单因素实验和响应面Box-Behnken设计实验筛选并优化莲子心总黄酮的多元醇超声辅助提取工艺，对该工艺获得的莲子心提取物进行了安全性及体外抗氧化、抑菌和抗敏活性的测定。结果表明，在提取溶剂为1,2-己二醇，溶剂体积分数87%，料液比3.6:100（g:mL），提取时间39 min的条件下，莲子心总黄酮得率为1.80%±0.08%，且其质量浓度在1.2 g/L以下时无刺激性，对DPPH和ABTS自由基的半数抑制质量浓度分别为0.76和0.69 g/L，对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的最小抑菌质量浓度分别为0.56，1.12和0.14 g/L；且其对透明质酸酶的半数抑制质量浓度为0.35 g/L。该研究提供了一种简单、安全、高效的莲子心总黄酮提取工艺，在保证较高总黄酮得率的基础上兼顾其提取物的生物活性，为莲子心资源的开发提供了理论依据。

关键词: 莲子心, 响应面, 总黄酮, 抗氧化活性, 抑菌活性, 抗敏活性

Abstract:

Nelumbinis Plumula is a kind of medicinal and edible plant with great development potential. Single factor test and response surface test were used to screen and optimize the ultrasonic-assisted extraction process of total flavonoids from Nelumbinis Plumula by polyols, and the safety, in vitro antioxidant, antibacterial and anti-allergy activities of Nelumbinis Plumula extract were determined. Results show that under the conditions of extraction solvent 1,2-hexanediol, solvent volume fraction 87%, material-liquid ratio 3.6:100 （g:mL）, and extraction time 39 min, the yield of total flavonoids is 1.80%±0.08%. At 1.2 g/L, the Nelumbinis Plumula extract has no irritation, the half maximal inhibitory concentration of DPPH radical and ABTS radical is 0.76 and 0.69 g/L, respectively, and the minimum inhibitory concentration for Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa are 0.56, 1.12 and 0.14 g/L, respectively. Moreover, the half maximal inhibitory concentration of hyaluronidase is 0.35 g/L. This study provides an easy, safe and efficient method for extracting total flavonoids from Nelumbinis Plumula, which ensures the biological activity of the extract and a high yield of total flavonoids, and provides a theoretical basis for the development of Nelumbinis Plumula.

Key words: Nelumbinis Plumula, response surface, total flavonoids, antioxidant activity, antibacterial activity, anti-allergy activity

中图分类号:

TQ658

郝晴, 吴二敏, 毕永贤, 刘金俊, 孔德承, 成志伟. 莲子心总黄酮多元醇提取工艺筛选及其性能研究[J]. 日用化学工业（中英文）, 2023, 53(4): 398-407.

Hao Qing, Wu Ermin, Bi Yongxian, Liu Jinjun, Kong Decheng, Cheng Zhiwei. Study on screening extraction process of total flavonoids from Nelumbinis Plumula by polyols and its properties[J]. China Surfactant Detergent & Cosmetics, 2023, 53(4): 398-407.

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水平	因素
水平	A 1,2-己二醇体积分数/%	B 料液比	C 提取时间/min
-1	70	1:100	30
0	80	3:100	40
1	90	5:100	50

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	0.58	9	0.065	115.01	<0.000 1	***
A	0.14	1	0.14	246.19	<0.000 1	***
B	0.090	1	0.090	159.97	<0.000 1	***
C	8.646×10^-4	1	8.646×10^-4	1.54	0.254 6
AB	0.012	1	0.012	20.85	0.002 6	**
AC	3.696×10^-3	1	3.696×10^-3	6.58	0.037 2	*
BC	6.663×10^-4	1	6.663×10^-4	1.19	0.312 1
A²	0.053	1	0.053	93.65	<0.000 1	***
B²	0.21	1	0.21	372.74	<0.000 1	***
C²	0.045	1	0.045	79.38	<0.000 1	***
残差	3.931×10^-3	7	5.615×10^-4
失拟项	1.247×10^-3	3	4.158×10^-4	0.62	0.638 2	不显著
纯误差	2.683×10^-3	4	6.708×10^-4
总差	0.59	16

样品名称	sH	sL	sC	IS值	刺激分级
0.9%氯化钠	301	301	301	0	无刺激性
0.1 mol/L氢氧化钠	5	30	53	18.70	强刺激性
莲子心提取物	280	301	301	0.35	无刺激性