响应面法优化水解制备柞蚕丝素肽及其性能

doi:10.3969/j.issn.1001-1803.2021.07.005

摘要/Abstract

摘要：

以柞蚕丝素蛋白为原料,用磷酸水解方法制备柞蚕丝素肽。在单因素实验基础上,通过响应面法优化制备工艺,考察各因素对柞蚕丝素肽回收率的影响,并对该工艺条件制备的样品进行了性能分析。结果表明：柞蚕丝素肽最佳水解工艺条件为：柞蚕丝素质量分数9.93%、水解温度91.11 ℃、水解时间5.44 h,理论回收率为70.34%,经检验实际回收率可以达到69%,与理论值基本相符,建立了响应面分析法优化的柞蚕丝素肽水解制备工艺。柞蚕丝素肽性能实验结果表明,样品具有良好的吸湿保湿性、防晒性及抑菌性,说明柞蚕丝素肽在天然护肤品开发方面具有良好的应用价值。

关键词: 柞蚕, 丝素肽, 水解工艺, 响应面法

Abstract:

Tussah fibroin peptides were prepared from tussah silk fibroin by hydrolysis method with phosphoric acid. On the basis of single-factor test, the preparation process was optimized using the response surface methodology. The effects of various factors on the recovery of tussah fibroin peptides were investigated, and the performance of samples obtained under the optimized conditions was analyzed. The results show that the optimal hydrolysis conditions are as follows: the mass fraction of liquid material is 9.93%; the hydrolysis temperature is 91.11 ℃; the hydrolysis time is 5.44 h; the theoretical recovery rate is 70.34%. The actual recovery rate can reach 69% which is consistent with the theoretical level. The optimized hydrolysis technology for preparation of tussah fibroin peptides was established using response surface methodology. The performance experiments of tussah fibroin peptides show that the sample has good hygroscopic and moisturizing efficacy, and functions of sunscreen and bacteriostasis. Tussah silk peptides have high application value in the development of natural skin care products.

Key words: tussah, fibroin peptide, hydrolysis process, response surface method

中图分类号:

TQ658

米锐,李学军,马淑慧,孟楠,李树英,都兴范. 响应面法优化水解制备柞蚕丝素肽及其性能[J]. 日用化学工业, 2021, 51(7): 612-619.

Mi Rui,Li Xuejun,Ma Shuhui,Meng Nan,Li Shuying,Du Xingfan. Optimization of hydrolysis preparation of tussah fibroin peptides by response surface methodology and the performance thereof[J]. China Surfactant Detergent & Cosmetics, 2021, 51(7): 612-619.

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水平	因素
水平	水解温度/℃ （A）	柞蚕丝素质量分数/% （B）	水解时间/h （C）
-1	85	9.5	3
0	90	10	5
1	95	10.5	7

方差来源	平方和	自由度	均方	F值	P值
模型	2 190.25	9	243.36	53.54	<0.000 1
A	786.06	1	786.06	172.95	<0.000 1
B	454.51	1	454.51	100.00	<0.000 1
C	137.78	1	137.78	30.31	0.000 9
AB	32.49	1	32.49	7.15	0.031 8
AC	16.40	1	16.40	3.61	0.099 3
BC	8.12	1	8.12	1.79	0.223 1
A²	321.26	1	321.26	70.68	<0.000 1
B²	229.63	1	229.63	50.52	0.000 2
C²	127.83	1	127.83	28.13	0.001 1
残差	31.82	7	4.55
失拟	25.13	3	8.38	5.01	0.076 8
纯误差	6.69	4	1.67
合计	2 222.06	16
相关系数r²	0.985 7
调整确定系数r²_adj	0.967 3
变异系数	2.13%