广藿香精油抗炎祛痘功效研究

doi:10.13218/j.cnki.csdc.2017.05.007

摘要/Abstract

摘要： 采用气相色谱-质谱联用技术对广藿香精油成分进行分析。通过细胞毒性实验确定广藿香精油对人急性单核细胞(THP-1)的安全质量浓度。利用痤疮丙酸杆菌(P.acnes)刺激THP-1建立的特异性痤疮炎症细胞模型考察广藿香精油对炎症因子IL-1β的影响。通过荧光实时定量逆转录聚合酶链反应(qRT-PCR)初步探讨广藿香精油抗炎祛痘的作用机制。研究结果表明:广藿香精油在最大安全质量浓度12.5 mg/L时,对炎症细胞模型中P.acnes活性影响较小,对IL-1β的分泌具有明显的抑制作用(抑制率66.54%),可显著降低细胞内Toll样受体2(TLR2) mRNA的转录(抑制率73.73%),从而起到抑制炎症信号转导和继发性炎症介质生成的作用。

关键词: 化妆品添加剂, 广藿香精油, 抗炎祛痘, 痤疮丙酸杆菌

Abstract: Chemical composition of the essential oil from Pogostemon cablin (Blanco) Benth.(EOP) was quantitatively analyzed by gas chromatography-mass spectrometry (GC-MS).The safe mass concentration of the EOP to Human Acute Mononuclear Cells (THP-1) was determined via cytotoxicity screening test.An inflammatory acne model was established by stimulating THP-1 with Propionibacterium acnes (P.acnes).Subsequently,the effect of the EOP on the expression of proinflammatory cytokine IL-1β was assessed.The anti-acne mechanism of the EOP was examined by qRT-PCR.Results show that at maximum safe mass concentration of 12.5 mg/L of the EOP,the influence on the activity of P.acnes is rather small and the secretion of IL-1β is significantly inhibited by a rate of 66.54%.The EOP also significantly reduces the mRNA level of Toll-like receptor 2 (TLR2) by 73.73%.Thereby,the EOP inhibits proinflammatory signal transduction and production of secondary inflammatory mediators.

Key words: cosmetic additive, essential oil from Pogostemon cablin, anti-acne, Propionibacterium acnes

中图分类号:

TQ658

王玥, 郭苗苗, 施雁勤, 潘仙华, 卢艳花, 曹平. 广藿香精油抗炎祛痘功效研究[J]. 日用化学工业, 2017, 47(5): 272-276.

WANG Yue, GUO Miao-miao, SHI Yan-qin, PAN Xian-hua, LU Yan-hua, CAO Ping. Anti-acne efficacy of essential oil from Pogostemon cablin (Blanco) Benth.[J]. China Surfactant Detergent & Cosmetics, 2017, 47(5): 272-276.

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