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日用化学工业（中英文）

日用化学工业 ›› 2021, Vol. 51 ›› Issue (7): 655-661.doi: 10.3969/j.issn.1001-1803.2021.07.011

• 开发与应用 • 上一篇    下一篇

大麻二酚的抗炎和抑菌活性研究

刘娟1,余思宜1,翟文丽1,2,李国庆1,李学涛3,刘继涛1,*()   

  1. 1.云南白药集团股份有限公司,云南 昆明 650500
    2.东亚肌肤健康研究中心,北京 100089
    3.辽宁中医药大学 药学院,辽宁 大连 116600
  • 收稿日期:2020-10-27 修回日期:2021-07-04 出版日期:2021-07-22 发布日期:2021-07-23
  • 通讯作者: 刘继涛
  • 基金资助:
    云南省科技计划项目(2108ZF005)

Investigation on the anti-inflammatory and antimicrobial activity of cannabidiol

Liu Juan1,Yu Siyi1,Zhai Wenli1,2,Li Guoqing1,Li Xuetao3,Liu Jitao1,*()   

  1. 1. Yunnan Baiyao Group Co., Ltd., Kunming, Yunnan 650500, China
    2. East Asia Skin Health Research Center, Beijing 100089, China
    3. School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning 116600, China
  • Received:2020-10-27 Revised:2021-07-04 Online:2021-07-22 Published:2021-07-23
  • Contact: Jitao Liu

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摘要：

采用CCK-8法考察大麻二酚（CBD）对HaCat细胞和RAW264.7细胞相对细胞活性的影响。采用LPS诱导的HaCat细胞和RAW264.7细胞炎症模型考察CBD对炎症因子分泌的抑制作用。采用抑菌环和抑菌率等实验方法对CBD抑制厌氧菌痤疮丙酸杆菌（P.acnes）的效果进行测试。结果表明,CBD浓度低于20 μmol/L时,其对正常/炎症HaCat细胞以及正常/炎症RAW264.7细胞均无细胞毒性,也不会促进相关细胞的增殖分化;CBD对炎症RAW264.7模型中TNF-α具有良好的抑制作用,10 μmol/L浓度下与阳性对照地塞米松（DEX）效果相当,对两种炎症模型中IL-6均有显著抑制作用,20 μmol/L浓度下对两种炎症模型中IL-1β均有显著抑制作用,浓度为10、20 μmol/L时对两种炎症模型中IFN-γ均有显著抑制作用,表明其具有良好的抗炎活性;将CBD乳液作用于 P.acnes,实验结果显示CBD浓度在20 μmol/L及以上时对 P.acnes有显著抑制作用。此外,抑菌率实验结果表明,CBD浓度为80 μmol/L时对 P.acnes的抑菌率高达99%,表明其具有良好的抑菌功效。

关键词: 大麻二酚, 抗炎, 痤疮丙酸杆菌, 抑菌

Abstract:

CCK-8 assay was performed to evaluate the effect of cannabinol （CBD） on the relative cell activity of HaCat cells and RAW264.7 cells. The inhibitory effect of CBD on the secretion of inflammatory cytokines in HaCat cells and RAW264.7 cells induced by LPS was investigated. The inhibitory effect of CBD on anaerobe Propionibacterium acnes（P.acnes） was tested by the means of inhibition ring and inhibition rate. The results show that when the concentration is lower than 20 μmol/L, CBD presents no cytotoxicity to normal/inflammatory HaCat cells and normal/inflammatory RAW264.7 cells, nor does it promote the proliferation and differentiation of the cells. CBD shows a good inhibitory effect on TNF-α in inflammatory RAW264.7 cell model, and its effect is equivalent to the positive control dexamethasone （DEX） at the concentration of 10 μmol/L. CBD shows significant inhibitory effect on IL-6 in both inflammatory cell models. At the concentration of 20 μmol/L, it presents significant inhibitory effect on IL-1β in both cell models; and presents significant inhibitory effect on IFN-γ in both cell models at the concentrations of 10 and 20 μmol/L, all indicating its good anti-inflammatory activity. The CBD emulsion was applied toP.acnes, and the results show that when the concentration of CBD is above 20 μmol/L, it shows significant inhibitory effect on P.acnes. In addition, the results of bacteriostatic rate test show that when the concentration of CBD is 80 μmol/L, its antibacterial rate against P.acnes is as high as 99%, indicating that it has a good antibacterial effect.

Key words: cannabidiol, anti-inflammatory, Propionibacterium acnes, antibacterial

中图分类号: 

  • TQ658