基于网络药理学探讨罗勒抗炎抗菌分子机制

doi:10.3969/j.issn.1001-1803.2023.01.008

摘要/Abstract

摘要：

利用网络药理学探究罗勒抗炎抗菌的作用机制，并通过体外试验进行初步验证。通过相关数据库筛选到罗勒的18种化合物和6种挥发油成分，作用靶点521个，获得抗炎抗菌相关靶点975个。使用Venny 2.1做药物和疾病靶点交集的韦恩图，得到88个罗勒抗炎抗菌靶点。构建蛋白互作PPI网络，并进行GO和KEGG富集分析。根据蛋白关联度可知核心靶点涉及TNF-α，IL-1β，IL-6等，主要通过对细菌的反应、对脂多糖的反应等信号通路发挥抗炎抗菌作用。体外细胞试验采用LPS及热灭活痤疮丙酸杆菌（Cutibacterium acnes，C.a）诱导的THP-1两种炎症细胞模型。采用ELISA法检测添加不同含量罗勒精油（Ocimum basilicum L. essential oil，OBEO）细胞的TNF-α，IL-1β、IL-6的分泌量。结果显示，体积分数为0.025%的OBEO能够抑制两种炎症损伤模型TNF-α，IL-1β，IL-6的表达；且OBEO对灭活C.a致THP-1细胞炎症损伤的抑制作用显著，分别降低TNF-α，IL-1β，IL-6的表达24%，45%，13%左右。

关键词: 罗勒, 抗炎抗菌, 网络药理, THP-1, 信号通路

Abstract:

Network pharmacology was used to explore the anti-inflammatory and antibacterial mechanism of basil, which was preliminarily verified by in vitro test. Through the relevant database, 18 compounds and 6 volatile oil components of basil are screened, and 521 action targets, 975 anti-inflammatory and antibacterial targets are obtained. The Venny 2.1 is used to make the Wayne diagram of the intersection of drugs and disease targets, and 88 basil anti-inflammatory and antibacterial targets are obtained. The protein-protein interaction PPI network is constructed and enriched by go and KEGG. According to the protein correlation degree, the core target involving TNF-α, IL-1 β and IL-6 plays an anti-inflammatory and antibacterial role mainly through the signal pathways such as response to bacteria and response to lipopolysaccharide. In vitro, two inflammatory cell models of THP-1 induced by LPS and heat inactivated Cutibacterium acnes （C.a） were used to detect TNF in cells supplemented with different concentrations of Ocimum basilicum L. essential oil （OBEO） by TNF-α, IL-1 β, IL-6 secretion. The results show that OBEO at a volume fraction of 0.025% is able to inhibit the expression of TNF-α, IL-1β, and IL-6 in both inflammatory injury models. And the inhibitory effect of OBEO on inactivated C.a caused inflammatory injury in THP-1 cells is significant, reducing the expression of TNF-α, IL-1β, and IL-6 by about 24%, 45%, and 13%, respectively.

Key words: Ocimum basilicum L., anti-inflammatory and antibacterial, network pharmacology, THP-1, pathway

中图分类号:

TQ658

孙锦月, 武楠楠, 张瑜, 何华名, 何聪芬. 基于网络药理学探讨罗勒抗炎抗菌分子机制[J]. 日用化学工业（中英文）, 2023, 53(1): 54-61.

Sun Jinyue, Wu Nannan, Zhang Yu, He Huaming, He Congfen. Molecular mechanism of anti-inflammatory and antibacterial of Ocimum basilicum L. based on network pharmacology[J]. China Surfactant Detergent & Cosmetics, 2023, 53(1): 54-61.

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