基于网络药理学的化妆品原料粉防己抗敏作用机制分析

doi:10.3969/j.issn.2097-2806.2024.03.009

摘要/Abstract

摘要：

采用网络药理学方法探索化妆品原料粉防己对敏感肌肤的抗敏作用机制。通过TCMSP、中国知网、PubMed等数据库收集粉防己的主要化学成分，并利用SwissTargetPrediction预测其靶点，与从Genecards数据库获得的疾病靶点取交集后采用String数据库构建蛋白质相互作用（PPI）网络，采用Cytoscape3.9.1软件分析获得核心靶点，再运用DAVID在线数据库对核心靶点进行基因GO功能分析和KEGG通路富集分析，Cytoscape3.9.1软件构建粉防己活性成分-靶点交互网络。共筛选出粉防己潜在活性成分50个及相应靶点682个，敏感肌肤疾病相关靶点2 389个，交集靶点295个，其中核心靶点39个。GO富集分析结果显示，39个核心靶点主要涉及蛋白磷酸化、信号转导、转录调控、基因表达等多种生物学过程。KEGG通路富集分析结果显示，粉防己发挥抗敏作用可能与VEGF信号通路、HIF-1信号通路、ErbB信号通路、PI3K-Akt信号通路密切相关。粉防己中荷包牡丹碱、千金藤素、防己诺林碱等活性成分可能是通过作用于JAK2、JAK3、SYC等靶点发挥抗敏作用，体现了粉防己多成分-多靶点-多通路的作用特点。

关键词: 网络药理学, 化妆品, 粉防己, 抗敏机制

Abstract:

Explore the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra on sensitive skin using a network pharmacological approach. The main chemical components of Stephania tetrandra were collected through TCMSP, CNKI, PubMed and other databases, and their targets were predicted using Swiss Target Prediction, intersected with the disease targets obtained from Genecards database and then used String database to construct protein interaction （PPI） network. Cytoscape3.9.1 software was used to analyze the core targets, and the DAVID online database was used to perform GO functional analysis and KEGG pathway enrichment analysis on the core targets. The results of GO enrichment analysis show that the 39 core targets are mainly involved in various biological processes such as protein phosphorylation, signal transduction, transcriptional regulation and gene expression. The results of KEGG pathway enrichment analysis show that the anti-sensitive skin effect of Stephania tetrandra may be closely related to VEGF signaling pathway, HIF-1 signaling pathway, ErbB signaling pathway and PI3K-Akt signaling pathway. The active ingredients in Stephania tetrandra, such as Dicentrined, Cepharanthine and Fangchinoline, may exert their anti-sensitive skin effects by acting on the targets of JAK2, JAK3 and SYC, reflecting the multicomponent-multitarget-multipathway action characteristics of Stephania tetrandra.

Key words: network pharmacology, cosmetics, Stephania tetrandra, sensitive skin

中图分类号:

TQ658

毕武, 潘小红, 涂晓琴, 殷帅, 孙辉. 基于网络药理学的化妆品原料粉防己抗敏作用机制分析[J]. 日用化学工业（中英文）, 2024, 54(3): 305-312.

Wu Bi, Xiaohong Pan, Xiaoqin Tu, Shuai Yin, Hui Sun. Analysis of the mechanism of anti-sensitive skin effect of cosmetic raw material Stephania tetrandra based on network pharmacology[J]. China Surfactant Detergent & Cosmetics, 2024, 54(3): 305-312.

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序号	化合物名称	Pubchem/ Chemspider ID	序号	化合物名称	Pubchem/Chemspider ID
1	Argentinine	8261415	21	Homoaromaline	9851833
2	Capaurine	9759	22	Isoboldine	98369
3	Cassameridine	12302502	23	Isoscoulerine	188442
4	Cassythicine	442194	24	Isotetrandrine	457825
5	Cepharanthine	320333	25	Juziphine	24692115
6	Coclaurine	248088	26	l-Stepholidine	6917970
7	Corydalmine	161655	27	Magnoflorine	73337
8	Corytuberine	160500	28	Methylfangchinoline	14753650
9	Cyclanoline	3082134	29	N-Methylcoclaurine	389483
10	Cycleanine	108295	30	Nantenine	197001
11	Cycleanorine	3082494	31	Norcepharanthine	181384
12	Dehydrocrebanine	149600	32	Norfangchinoline	10531531
13	Dehydrodicentrine	1084326	33	Obaberine	90581
14	Dehydrodiscretamine	102316663	34	Oblongine	138294
15	Dicentrine	101300	35	Oxofangchirine	128108
16	Fangchinoline	66173	36	Oxonantenine	3084224
17	Fenfangjine A	102121497	37	Protosinomenine	102067840
18	Fenfangjine B	102121498	38	Reticuline	9816
19	Fenfangjine C	102121829	39	Roemerine	119204
20	Fenfangjine F	8956505	40	Scoulerine	22955
41	Stephaflavone A	12087758	46	Tazopsine	11653181
42	Stephaflavone B	5324248	47	Tetrahydrojatrorrhizine	185605
43	Stepharanine	10358881	48	Tetrahydropalmatine	5224
44	Stephenanthrine	161379	49	Tetrandrine	73078
45	Stephibaberine	9938773	50	Fenfangjine G	102004748

通路分类（大类）	通路分类（小类）	通路名称
Environmental information processing	Signal transduction	VEGF signaling pathway
	Signal transduction	HIF-1 signaling pathway
	Signal transduction	ErbB signaling pathway
	Signal transduction	PI3K-Akt signaling pathway
	Endocrine system	Prolactin signaling pathway
	Cellular community-eukaryotes	Focal adhesion
	Endocrine system	Relaxin signaling pathway
	Immune system	Chemokine signaling pathway
	Nervous system	Neurotrophin signaling pathway
Human diseases		Pathways in cancer
		AGE-RAGE signaling pathway in diabetic complications
		Proteoglycans in cancer
		Endocrine resistance
		Chemical carcinogenesis-receptor activation
		Kaposi sarcoma-associated herpesvirus infection
		Hepatitis B
		Pancreatic cancer
		EGFR tyrosine kinase inhibitor resistance
		Lipid and atherosclerosis
		Human cytomegalovirus infection