前体离子扫描超高效液相色谱-三重四级杆串联质谱法快速筛查化妆品中非法添加的磺胺类药物

doi:10.3969/j.issn.2097-2806.2024.02.014

摘要/Abstract

摘要：

磺胺类药物（SAs）为化妆品中禁用物质。采用超高效液相色谱-三重四级杆/复合线性离子阱串联质谱（UPLC-QTrap），利用前体离子-增强子离子扫描（PIS-IDA-EPI）模式，用于快速筛查化妆品中非法添加的SAs。选择三个碰撞诱导解离形成的产物离子m/z 92，m/z 108，m/z 156，对12种化妆品基质中的20个SAs进行PIS-IDA-EPI扫描分析，检出限LODs为0.05~0.1 μg/g，该方法在12批次化妆品加标样品中进行了验证，并测定了60批次化妆品样品，以确保其有效性、灵敏度和选择性。实验结果表明：UPLC-QTrap结合PIS-IDA-EPI采集模式是一种有效的筛查方法，并成功应用于化妆品样品中目标和非目标SAs的快速筛查。

关键词: 磺胺类药物, 化妆品, 三重四级杆/复合线性离子阱质谱（QTrap）, 前体离子扫描（PIS）

Abstract:

Sulfonamides （SAs） are prohibited substances in cosmetics. An ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry （UPLC-QTrap） method using precursor ion-information dependent acquisition-enhanced product ion （PIS-IDA-EPI） scanning mode was developed to screen SAs in cosmetics. Twenty SAs were investigated to identify their common product ions formed by collision-induced dissociation. Three common positive product ions, m/z 92, m/z 108, m/z 156 are selected for PIS analysis. Limits of detection （LODs） are 0.05-0.1 μg/g for all 20 SAs in 12 kinds of cosmetic matrix. Sixty samples are analyzed. An effective screening method using UPLC-QTrap with PIS-IDA-EPI acquisition mode is developed and successfully applied to screen for the targeted and untargeted SAs in cosmetic samples.

Key words: sulfonamides （SAs）, cosmetics, triple quadrupole/composite linear ion trap mass spectrometry （QTrap）, precursor ion scan （PIS）

中图分类号:

TQ658

芦丽, 方方, 冯有龙, 曹玲. 前体离子扫描超高效液相色谱-三重四级杆串联质谱法快速筛查化妆品中非法添加的磺胺类药物[J]. 日用化学工业（中英文）, 2024, 54(2): 216-223.

Li Lu, Fang Fang, Youlong Feng, Ling Cao. Screening for illegal addition of sulfonamides in cosmetic products using ultra-performance liquid chromatographytriple quadrupoletandem mass spectrometry with precursor ion scanning[J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 216-223.

图/表 6

图1

图2

图3

表1

磺胺类药物的前体离子、碎片离子和检出限（CE=25 eV）"

序号	化合物	英文名称	保留时间/min	前体离子（m/z）	PIS模式下子离子			碎片离子	LOD/（μg/g）
序号	化合物	英文名称	保留时间/min	前体离子（m/z）	92.0	108.0	156.0	碎片离子	LOD/（μg/g）
1	磺胺胍	Sulfaguanidine	1.215	215.1	√	√	√	191.0，172.9，120.1	0.05
2	磺胺醋酰钠	Sulfacetamide sodium	3.228	237.0	√	√	√	227.5，220.1，131.1	0.1
3	磺胺二甲基异嘧啶	Sulfisomidine	3.990	279.1	√	√	√	227.5，204.0，186.0，124.1	0.05
4	磺胺吡啶	Sulfapyridine	4.662	250.1	√	√	√	227.5，217.4，184.1	0.05
5	磺胺甲嘧啶	Sulfamerazine	4.915	265.1	√	√	√	227.5，190.0，110.1	0.05
6	磺胺二甲噁唑	Sulfamoxol	5.244	268.1	√	√	√	227.5，192.0，113.1	0.05
7	磺胺甲二唑	Sulfamethizole	5.493	271.0	√	√	√	227.5	0.05
8	磺胺甲氧哒嗪	Sulfamethoxypyridazine	5.576	281.1	√	√	√	247.5，227.5，215.1，126.1	0.05
9	磺胺二甲嘧啶	Sulfamethazine	5.510	279.1	√	√	√	227.5，204.0，124.1，186.0	0.05
10	磺胺对甲氧嘧啶	Sulfameter	5.576	281.1	√	√	√	227.5， 126.1	0.05
11	磺胺氯哒嗪	Sulfachloropyridazine	6.158	285.0	√	√	√	227.5，207.1，130.1，110.1	0.05
12	磺胺甲氧吡嗪	Sulfalen	6.050	281.1	√	√	√	227.5，215.1，199.6，188.0	0.05
13	磺胺间甲氧嘧啶	Sulfamonomethoxine	5.993	281.1	√	√	√	227.5，215.1，175.5	0.05
14	磺胺甲噁唑	Sulfamethoxazole	6.325	254.1	√	√	√	227.5，188.1，147，1	0.05
15	周效磺胺	Sulfadoxine	6.408	311.1	√	√	√	245.1，140.0，227.5	0.05
16	磺胺二甲异噁唑	Sulfisoxazole	6.574	268.1	√	√	√	227.5，175.0，140.0，113.1	0.05
17	苯甲酰磺胺	Sulfabenzamide	6.826	277.1	√	√	√	227.5，197.2	0.05
18	磺胺苯吡唑	Sulfaphenazole	7.075	315.1	√	√	√	222.0，188.1，124.1	0.05
19	磺胺二甲氧嗪	Sulfadimethoxine	6.992	311.1	√	√	√	245.1，230.1，218.0	0.05
20	磺胺喹噁啉	Sulfaquinoxaline	7.004	301.1	√	√	√	235.1，227.5，208.0	0.05

表1

图4

图5

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