甘草次酸对金黄地鼠皮脂腺斑影响的实验研究

doi:10.3969/j.issn.1001-1803.2022.06.007

摘要/Abstract

摘要：

以甘草次酸为活性成分,通过3T3-L1脂肪前体细胞模型测试不同质量浓度的甘草次酸对3T3-L1增殖及分泌甘油三酯（Triglyceride,TG）成分的影响,进一步采用金黄地鼠动物模型实验测定不同剂量的甘草次酸对金黄地鼠皮脂腺斑影响,并初步分析其可能的作用机制。结果显示,在25~100 μg/mL质量浓度范围内不抑制3T3-L1细胞增殖,在质量浓度为50~100 μg/mL时具有显著抑制3T3-L1细胞分泌TG（P<0.01）并呈浓度依赖性。给予金黄地鼠药物30 d后,与空白对照组、基质组（50%丙二醇水溶液）相比,甘草次酸在设定质量浓度下显著降低金黄地鼠腹部双侧皮脂腺斑面积,减少皮脂腺厚度及数量,抑制皮脂腺生长进而减少皮脂腺分泌皮脂中的甘油三酯成分,甘草次酸在100 μg/mL质量浓度下主要通过显著调控皮脂腺组织中SREBP-1C（P<0.001）,TG（P<0.01）,K6（P<0.01）,K16（P<0.01）,IL-6（P<0.05）指标的表达,即抑制皮脂腺组织中SREBP蛋白活性,抑制皮脂合成及分泌TG成分的含量,进一步降低角质形成细胞中角蛋白K6及K16表达,发挥调控皮脂腺生长及皮脂中甘油三酯成分分泌的作用。

关键词: 甘草次酸, 皮脂腺斑, 金黄地鼠, 抗炎

Abstract:

This experiment preliminarily confirmed that glycyrtinic acid at the mass concentration of 50-100 μg/mL could significantly inhibit the secretion of triglyceride by 3T3-L1 cells （P<0.01） in a mass concentration-dependent manner. 3T3-L1 cells, as preadipocytes, continue to increase adipocyte proliferation and triglyceride accumulation under exogenous stimulation. They differentiate into mature adipocytes and form adipose tissue. Glycyrrhetinic acid can inhibit the proliferation of preadipocytes and reduce the accumulation of triglycerides, indicating that glycyrrhetinic acid has the effect of inhibiting lipid synthesis and metabolism at the cellular level. Further, through the golden hamsters model, it is confirmed that glycyrrhetic acid at the set mass concentration significantly reduces the area of bilateral sebaceous gland on the abdomen of golden hamsters, reduces the thickness and number of sebaceous glands, inhibites the growth of sebaceous glands and thus reduces the abnormal excessive secretion of sebaceous glands. At the mass concentration of 100 μg/mL, glycyrrhetinic acid significantly regulates the expression of SrebP-1C （P<0.001）, TG （P<0.01）, K6 （P<0.01）, K16 （P<0.01） and IL-6 （P<0.05） in sebaceous glands. It inhibits the SREBP protein activity in sebaceous gland tissue, inhibits sebum synthesis and secretion of triglyceride composition content, and further reduces the cutin formation and keratin K6, K16 expression in cells, which play a role in regulating sebaceous gland growth and oil secretion. Abnormal sebum secretion from sebaceous glands can induce skin health problems such as acne, skin barrier dysfunction and specific dermatitis. Through the above experimental studies, it has been preliminarily confirmed that the single use of glycyrrhetinic acid can effectively inhibit the growth of sebaceous gland spots in golden hamsters and reduce sebum secretion and inflammatory reaction. These results suggest that glycyrrhetinic acid may play a role in regulating oily skin, inhibiting acne, improving skin barrier function and specific dermatitis, and can be used as an active ingredient in skin surgery drugs and functional daily cosmetics that relieve acne and skin inflammation.

Key words: glycyrrhetinic acid, sebaceous gland, golden hamsters, anti-inflammatory

中图分类号:

TQ658

何益飞,鲁旺旺,马诗经,刘冠廷,王梦萍,杜志云. 甘草次酸对金黄地鼠皮脂腺斑影响的实验研究[J]. 日用化学工业, 2022, 52(6): 620-625.

He Yifei,Lu Wangwang,Ma Shijing,Liu Guanting,Wang Mengping,Du Zhiyun. Experimental study on the effect of glycyrrhetinic acid on sebaceous gland of golden hamsters[J]. China Surfactant Detergent & Cosmetics, 2022, 52(6): 620-625.

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实验组	甘草次酸质量浓度/（μg/mL）	3T3-L1细胞活性/%	毒性分级
空白对照组		102.10±1.05	0
甘草次酸组	25	99.13±1.36	1
	50	98.58±1.76	1
	100	97.69±1.07	1
	125	71.99±0.55	3

实验组	甘草次酸质量浓度/（μg/mL）	TG含量/（μmol/L）
空白对照组		24.63±3.51
模型组		49.63±4.38^###
甘草次酸组	25	46.53±4.09
	50	33.51±3.80^**
	100	26.07±3.12^***

组别	给药前		给药后
组别	左侧/mm²	右侧/mm²	左侧/mm²	右侧/mm²
空白对照组	37.57±3.11	35.88±1.91	58.92±1.90 ^a	57.50±3.82 ^a
基质组	39.02±4.48	36.24±3.66	59.71±1.17 ^b	61.76±2.81 ^b
甘草次酸低剂量（50 μg/mL）组	36.83±2.95	35.96±4.80	47.20±2.80 ^{**, ##}	49.80±1.79 ^{**, ##}
甘草次酸高剂量（100 μg/mL）组	38.05±2.36	37.48±5.07	45.71±1.94 ^{***, ###}	44.16±2.32 ^{***, ###}

实验组	皮脂腺厚度/mm	皮脂腺数量/个
空白对照组	0.872±0.080	42.00±5.48
基质组	0.838±0.068	41.00±3.56
甘草次酸低剂量（50 μg/mL）组	0.561±0.084^**,##	31.25±2.75^**,##
甘草次酸高剂量（100 μg/mL）组	0.432±0.093^***,###	23.00±2.58^***,###

实验组	SREBP-1C/（pg/mL）	TG/（μmol/mL）	K6/（pg/mL）	K16/（pg/mL）	IL-6/（pg/mL）
空白对照组	876.02±25.81	1.88±0.24	24.82±2.39	56.01±4.21	27.31±2.71
基质组	849.38±32.27	1.83±0.37	26.36±1.18	59.87±2.08	30.81±4.01
甘草次酸低剂量（50 μg/mL）组	615.77±19.63^**,##	1.32±0.13^*,#	20.27±2.09	43.29±4.18^**,##	24.27±2.20
甘草次酸高剂量（100 μg/mL）组	528.03±35.1^***,###	1.06±0.22^**,##	18.06±0.95^**,##	42.05±1.92^**,##	20.15±2.42^*,#