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日用化学工业（中英文）

日用化学工业 ›› 2014, Vol. 44 ›› Issue (1): 31-34.doi: 10.13218/j.cnki.csdc.2014.01.008

• 开发与应用 • 上一篇    下一篇

生物防晒剂活酵母细胞衍生物制备中酶解条件的优化

陈玲林1, 代彦2, 赵华1   

  1. 1.天津科技大学 生物工程学院,天津 300457;
    2.诺和诺德(中国)制药有限公司,天津 300457
  • 收稿日期:2013-06-28 发布日期:2019-04-09
  • 通讯作者: 赵 华,教授,电话:(022)60601396,E-mail:zhaohua@tust.edu.cn。
  • 作者简介:陈玲林(1988-),女,江苏盐城人,硕士研究生,电话:(022)60602126。

Optimization of enzymolysis conditions in preparation of biological sunscreen live yeast cell derivative

CHEN Ling-lin1, DAI Yan2, ZHAO Hua1   

  1. 1.College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China;
    2.Novo Nordisk (China) Pharmaceuticals Co.,Ltd,Tianjin 300457,China
  • Received:2013-06-28 Published:2019-04-09

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摘要： 研究了紫外照射培养后蛋白酶添加量、酶解温度、酶解时间及pH等酵母菌酶解条件对酵母水解液的紫外吸收性能的影响,并将活酵母细胞衍生物应用于防晒护肤品中。实验结果表明,活酵母细胞衍生物的优化制备条件为:质量分数10%酵母菌液中添加2%的蛋白酶,1%的纤维素酶,pH=6,52 ℃酶解26 h。所制得的活酵母细胞衍生物作为防晒护肤品添加剂,使其SPF值最大可提高128.2%。

关键词: 化妆品添加剂, 活酵母细胞衍生物, 防晒

Abstract: Effects of yeast enzymolysis conditions,such as dosage of UV irradiation cultivated protease,temperature,pH and time of the enzymolysis on the UV absorption performance of the yeast enzymolysis product solution were studied.Further,the live yeast cell derivative was applied in sun screening skin care cosmetic product.Experimental results showed that the optimum conditions for preparation of live yeast cell derivative are:addition of 2% of protease and 1% of cellulase into 10% (mass fraction) yeast solution,pH=6,temperature 52 ℃ and enzymolysis time of 26 h.The live yeast cell derivative can be used as additive in sun screening skin care cosmetics so as to increase maximum sun protection factor of the cosmetics by 128.2%.

Key words: additive of cosmetics, live yeast cell derivative, sun screening

中图分类号: 

  • TQ658.2