藏荆芥提取物对酪氨酸酶活性及其动力学研究

doi:10.3969/j.issn.1001-1803.2019.05.007

日用化学工业 ›› 2019, Vol. 49 ›› Issue (5): 315-319.doi: 10.3969/j.issn.1001-1803.2019.05.007

藏荆芥提取物对酪氨酸酶活性及其动力学研究

王飞飞¹,王敏竹¹,王晗¹,黄山¹,旦增江白²,李斌¹()

^1. 青岛科技大学化工学院,山东青岛 266042
^2. 班戈卓攀健康产业有限责任公司,西藏那曲 852500

收稿日期:2018-09-08 修回日期:2019-04-30 出版日期:2019-05-22 发布日期:2019-05-24
通讯作者: 李斌
作者简介:王飞飞（1996-）,女,河北人,电话：18233670964,E-mail：1548028099@qq.com。
基金资助:
青岛科技大学校级大学生创新创业训练计划项目(201710426109);青岛科技大学校级大学生创新创业训练计划项目(2018020013);国家自然科学基金资助项目(81360686);西藏自治区2017年科技孵化器项目

The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosinase activity and kinetics

WANG Fei-fei¹,WANG Min-zhu¹,WANG Han¹,HUANG Shan¹,DANZENG Jiang-bai²,LI Bin¹()

^1. School of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China
^2. Bangor Zhuopan Health Industry Co., Ltd., Naqu, Tibet 852500, China

Received:2018-09-08 Revised:2019-04-30 Online:2019-05-22 Published:2019-05-24
Contact: Bin LI

摘要/Abstract

摘要：

以L-酪氨酸和L-多巴为底物,采用酶促反应动力学方法研究藏荆芥提取物对酪氨酸单酚酶和二酚酶活性的影响以及抑制动力学。结果表明,藏荆芥提取物对酪氨酸单酚酶和二酚酶均有抑制作用,单酚酶和二酚酶活力下降50%的藏荆芥提取物质量浓度（IC₅₀）分别为0.138 8和0.533 2 g/L。藏荆芥提取物对酪氨酸二酚酶的抑制作用属于可逆过程,Lineweaver-Burk图显示其作用类型表现为竞争性抑制,抑制常数为504.846 mg/L。藏荆芥提取物对酪氨酸酶有明显的抑制作用,具有开发成天然酪氨酸酶抑制剂的潜力。

关键词: 化妆品添加剂, 藏荆芥提取物, 酪氨酸酶, 动力学, 可逆竞争性抑制

Abstract:

The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosine monophenolase and diphenolase activity and inhibition kinetics were studied by enzymatic reaction using L-tyrosinase and L-DOPA as substrates. Experimental results show that the extract of Nepeta angustifolia C.Y.Wu, has inhibitory effect on tyrosine monophenolase and diphenolase, and the concentrations of the extract of Nepeta angustifolia C.Y.Wu leading to 50% decrease in monophenolase and diphenolase activity（IC₅₀）are calculated to be 0.138 8 and 0.533 2 g/L, respectively. The inhibition of tyrosine diphenolase by the extract of Nepeta angustifolia C.Y.Wu is a reversible process. The Lineweaver-Burk plots show that the type of action is competitive inhibition with an inhibition constant of 504.846 mg/L. The extract of Nepeta angustifolia C.Y.Wu has a significant inhibitory effect on tyrosinase and has the potential to be developed into a natural tyrosinase inhibitor.

Key words: cosmetic additive, extract of Nepeta angustifolia C.Y.Wu, tyrosinase, kinetics, reversible competitive inhibition

中图分类号:

TQ658

王飞飞,王敏竹,王晗,黄山,旦增江白,李斌. 藏荆芥提取物对酪氨酸酶活性及其动力学研究[J]. 日用化学工业, 2019, 49(5): 315-319.

WANG Fei-fei,WANG Min-zhu,WANG Han,HUANG Shan,DANZENG Jiang-bai,LI Bin. The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosinase activity and kinetics[J]. China Surfactant Detergent & Cosmetics, 2019, 49(5): 315-319.

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参考文献 16

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ρ（藏荆芥提取物）/ （mg·L^-1）	回归方程	R²	K_m/（mg·L^-1）
0	y=17.217x+13.365	0.993 1	1.288
100	y=18.039x+15.567	0.998 4	1.159
200	y=26.436x+11.840	0.998 7	2.233
300	y=26.356x+15.165	0.991 1	1.738
400	y=22.401x+20.754	0.995 0	1.079
500	y=32.365x+15.592	0.983 2	2.076

藏荆芥提取物对酪氨酸酶活性及其动力学研究

The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosinase activity and kinetics

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反应液的组成	V/μL
反应液的组成	a	b	c	d
底物	10	10	10	10
溶剂	90	80	70	60
药品	–	–	20	20
酪氨酸酶	–	10	–	10

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