银耳多糖提取工艺优化及对人体细胞毒性检测

doi:10.13218/j.cnki.csdc.2016.02.009

摘要/Abstract

摘要： 以多糖提取率为指标,以料液比(g∶mL)、提取温度和提取时间为影响因素进行热水浸提银耳多糖的单因素试验,在此基础上应用Box-Behnken进行响应面分析。得到较佳工艺条件:料液比为1∶60,提取时间为5.57 h,提取温度为97.18 ℃,理论多糖提取率为20.52%,实际测得值为20.25%,说明该方法可靠。采用MTT(四甲基偶氮唑盐)实验和二氢乙啶(DHE)染色实验检测水提银耳多糖对人皮肤成纤维细胞(HSF)的毒性。MTT实验表明,银耳多糖溶液质量浓度在0~200 mg/L范围内HSF成活率为1,DHE染色实验结果为阴性,说明水提银耳多糖对HSF没有毒性。

关键词: 化妆品, 银耳多糖, 响应面法, MTT, 二氢乙啶染色

Abstract: Single factor tests for hot water extraction of Tremella fuciformis polysaccharide were conducted with polysaccharide yield as the criteria index as well as solid to solvent ratio (g∶mL),extraction time and extraction temperature as the influencing factors.Based upon the test results,a response surface analysis was derived according to Box-Behnken central composite experimental design.The optimal extraction conditions were obtained as following: solid to solvent ratio,1∶60;extraction time,5.57 h;extraction temperature,97.18 ℃.Under the above conditions,the yield of Tremella fuciformis polysaccharide reaches 20.52% while the yield obtained by quantitative analysis is 20.25%.Such comparison showed that the test method is reliable.Methyl thiazolyl tetrazolium (MTT) test method and dihydroethidium (DHE) staining test method were used for detection of toxicity to fibroblast of human skin (HSF).When the mass concentration of the polysaccharide is within range of 0-200 mg/L,the MTT test showed that the survival rate of HSF achieves 1 and the DHE staining test showed negative.Such results proved that the Tremella fuciformis polysaccharide has no cytotoxicity on HSF.

Key words: cosmetics, Tremella fuciformis polysaccharide, response surface analysis, MTT, DHE

中图分类号:

TQ658

段超,王昌涛. 银耳多糖提取工艺优化及对人体细胞毒性检测[J]. 日用化学工业, 2016, 46(2): 101-105.

DUAN Chao,WANG Chang-tao. Optimization of extracting Tremella fuciformis polysaccharide and detection of its toxicity to cells of human body[J]. China Surfactant Detergent & Cosmetics, 2016, 46(2): 101-105.

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