当期目录

2016年, 第46卷, 第2期　刊出日期：2016-02-22 上一期    下一期

基础研究

Select

季铵盐表面活性剂有机蒙脱土的制备及其苯酚吸附行为研究 Preparation of organo-montmorillonite modified with quaternary ammonium surfactant and its adsorption behavior for phenol 高艳丽,孙晨雅,齐丽云,方云 2016 (2):  61-65.  doi: 10.13218/j.cnki.csdc.2016.02.001 摘要 ( 263 )   PDF(860KB) ( 426 )   选用阳离子表面活性剂双十六烷基二甲基溴化铵(DHDMA)和Gemini型表面活性剂1,2-亚乙基-双(十六烷基二甲基溴化铵)(16-2-16)分别制备2种有机蒙脱土产品DHDMA-MMT和16-2-16-MMT。采用FT-IR,XRD和TGA进行产品结构表征;并对比研究了2种产品吸附水中苯酚的性能。实验结果表明,当表面活性剂负载量相当时,2种产品有机碳含量相近,而16-2-16-MMT对苯酚的吸附量和有机碳归一化吸附系数均显著大于DHDMA-MMT。2种产品的分子中均含有2条十六烷基碳链,16-2-16分子仅比DHDMA分子多1个季铵盐头基,表明季铵盐头基在有机蒙脱土吸附苯酚过程中具有重要的作用。 Cationic surfactant dihexadecyldimethylammonium bromide (DHDMA) and Gemini surfactant 1,2-ethylene bis(cetyldimethyl ammonium bromide) (16-2-16) were used separately to prepare two kinds of organo-montmorillonite DHDMA-MMT and 16-2-16-MMT.The products were characterized by FT-IR,X-ray diffraction (XRD) and thermal gravimetric analysis (TGA).Phenol adsorption behavior of the two kinds of product was investigated and compared.Experiment results demonstrated that the organic carbon content of the two kinds of product is close to each other at the same surfactant loadings while the adsorption amount of phenol and normalized organic carbon content adsorption coefficient of 16-2-16-MMT is remarkably higher than that of DHDMA-MMT.In view of that same carbon chains are containing in the molecule structure of the two kinds of product,and the structure difference of the two surfactants is only that 16-2-16 owns an extra quaternary ammonium headgroup as comparing with DHDMA,it is reasonable to conclude that the quaternary ammonium headgroup plays an important role in the process of adsorbing phenol from aqueous solution by the organo-montmorillonite. 参考文献 | 相关文章 | 计量指标

科技讲座

Select

氟表面活性剂和氟聚合物(Ⅱ)——环境与安全问题 Fluorinated surfactants and fluoropolymers (Ⅱ) Environmental and safety issues 邢航,陈现涛,肖进新 2016 (2):  66-74.  doi: 10.13218/j.cnki.csdc.2016.02.002 摘要 ( 282 )   PDF(1119KB) ( 319 )   主要从全氟辛烷磺酰氟/磺酸/磺酸盐(统称为PFOS)的生产、在环境中的存在和排放、接触和摄入、监管和减排、长距离迁移、生物累积和排泄、降解及毒性等8个方面对PFOS的环境与安全问题进行详细介绍;并就PFOS的安全性,从一个表面活性剂工作者的角度提出了自己的一点见解,旨在提高氟表面活性剂的应用价值。 The environmental and safety issues of perfluorooctane sulfonyl fluoride/perfluorooctane sulfonic acid/perfluorooctane sulfonates (PFOS) were introduced in detail,including their production,occurrence and emission in environment,exposure and intake to human being,supervision and emission reduction,long-distance transport,bioaccumulation and excretion,degradation and toxicity.Opinion with respect to safety issues of PFOS was also proposed in view of surfactant working field in order to upgrade their application value. 参考文献 | 相关文章 | 计量指标

Select

表面活性剂的性能与应用(ⅩⅩⅥ) ——表面活性剂在化妆品中的应用 Performance and applications of surfactants (ⅩⅩⅥ) Applications of surfactants in cosmetics 吴望波,赵莉,张华涛,徐宝财 2016 (2):  75-79.  doi: 10.13218/j.cnki.csdc.2016.02.003 摘要 ( 321 )   PDF(832KB) ( 450 )   介绍了化妆品中常用的表面活性剂。表面活性剂具有乳化、增溶、分散、起泡、润湿等作用,在各种化妆品中广泛应用。简要分析了生物表面活性剂、烷基聚葡糖苷表面活性剂、壳聚糖类表面活性剂等的研究进展。最后对表面活性剂在化妆品中的应用进行了总结和展望。 The commonly used surfactants in cosmetics were introduced.Surfactants are widely used in cosmetics on the basis of their emulsifying,solubilization,dispersion,foaming,wetting and other functions.Then,progress in research work field with respect to bio-surfactants,alkyl polyglycoside surfactants,chitosan surfactants et al.were briefed.Finally,the applications of surfactants in cosmetics were summarized and prospected. 参考文献 | 相关文章 | 计量指标

开发与应用

Select

非对称阳离子双子表面活性剂的合成和性能研究 Synthesis and performance of an asymmetric cationic Gemini surfactant 刘凡,许虎君 2016 (2):  80-83.  doi: 10.13218/j.cnki.csdc.2016.02.004 摘要 ( 277 )   PDF(857KB) ( 445 )   采用十六烷基二甲基叔胺和十八烷基二甲基叔胺分别与盐酸、环氧氯丙烷反应,制备得中间产物N-(3-氯-2-羟基)丙基-N,N-二甲基十六烷基氯化铵和N-(3-氯-2-羟基)丙基-N,N-二甲基十八烷基氯化铵。2种中间产物在碳酸钠催化下先后与乙二胺反应生成一种含长亲水链非对称阳离子双子表面活性剂(16-X-18)。采用IR和1H NMR对中间体和产物进行表征,采用电导率法研究了所制备的表面活性剂的表面活性。结果表明,所制备的非对称阳离子双子表面活性剂的Krafft点低于0 ℃,cmc在25 ℃时为2.935×10-5 mol/L,反离子结合度随温度的上升而降低。 Hexadecyl dimethyl tertiary amine and octadecyl dimethyl tertiary amine were separately used as starting material and to be reacted successively with hydrochloric acid and epichlorohydrin to prepare two intermediates,N-(3-chloro-2-hydroxy)propyl-N,N-dimethyl hexadecyl ammonium chloride and N-(3-chloro-2-hydroxy)propyl-N,N-dimethyl octadecyl ammonium chloride.Then ethylene di-amine reacted with the two intermediates successively in presence of sodium carbonate to obtain the product asymmetric Gemini surfactant (symbolized as 16-X-18).The intermediates and the product were characterized by IR and 1H NMR as well as the surface activities of 16-X-18 were measured by conductivity method.Results showed that Krafft point of the product is below 0 ℃,and its cmc at 25 ℃ is 2.935×10-5 mol/L.The degree of combination with the counter-ion decreases as the temperature rises. 参考文献 | 相关文章 | 计量指标

Select

配方组成及物化环境对烷基糖苷乳化体系液晶形成的影响 Influence of formulation ingredients and physicochemical environment on formation of liquid crystal in alkyl polyglucoside system 袁旻嘉,汪飞,章丽华,郭奕光 2016 (2):  84-88.  doi: 10.13218/j.cnki.csdc.2016.02.005 摘要 ( 367 )   PDF(3500KB) ( 516 )   系统地研究了配方组成及物化环境对烷基糖苷类乳化剂体系液晶形成的影响,探讨了可能的影响机理。配方组成主要包括4种不同种类的乳化剂、5种不同分子结构的助乳化剂、4种不同极性的油脂和4种不同分子结构的聚合物及其用量,物化环境主要包括pH及离子强度,在相同的实验条件下均质乳化获得液晶体系。实验结果表明:4种乳化剂中,花生醇葡糖苷所形成的液晶结构单元较多、尺寸较大,且结构规整;5种助乳化剂中,菊粉月桂基氨基甲酸酯可以有效促进液晶结构的形成,相对提高乳液稳定性;4种油脂中,白油所形成的液晶结构单元最多,尺寸较小且均匀;4种聚合物均可促进烷基糖苷类乳化剂液晶结构的形成,且随着聚合物质量分数的增加,液晶结构单元增多、尺寸更小且均匀;pH及离子强度对体系的液晶形成没有明显影响;然而,高离子强度(w(NaCl)>0.3%)会影响液晶乳液的稳定性。 The influences of formulation ingredients and physicochemical environment on the formation of liquid crystals in alkyl polyglucoside (APG) systems were studied systematically.Possible mechanism of forming of liquid crystal system was explored.The formulation ingredients included four kinds of APG emulsifier,five kinds of co-emulsifier with different molecular structure,four kinds of oil with different polarity and four kinds of polymer with different molecular structure.Meanwhile,different dosages of the above mentioned raw materials were discussed.The physicochemical environment included mainly pH value and ionic strength.Emulsions with liquid crystal were obtained under same experimental conditions via homogenizing.Results showed that,within the four kinds of emulsifier,that formed with arachyl alcohol has more amount of liquid crystals with bigger size and regular structure;within the five kinds of co-emulsifier,inulin amino lauryl formate can effectively promote the formation of liquid crystal structure,and increase the emulsion stability;within the four kinds of oil,that formed with white oil has the most amount,rather smaller size and homogenous liquid crystal structure units;the four kinds of polymer may promote the formation of liquid crystal structure,while as the increase of the polymer mass fraction,the liquid crystal unit structure is smaller in size and more homogeneous;pH value and ionic strength has no obvious influence on liquid crystal status,while high ionic strength (w(NaCl)>0.3%) would reduce stability of the liquid crystal emulsion. 参考文献 | 相关文章 | 计量指标

Select

季铵盐型洗手液的制备及其抗菌性能研究 Preparation and performance of a quaternary ammonium salt based antibacterial hand sanitizer 黎敏珊,郑成,毛桃嫣,周虹谷,欧志荣 2016 (2):  89-91.  doi: 10.13218/j.cnki.csdc.2016.02.006 摘要 ( 536 )   PDF(747KB) ( 355 )   通过对原料的选择和配方的优化,制备了一种季铵盐型抗菌洗手液。该洗手液配方(以质量分数计)由0.75%十二烷基聚氧乙烯醚硫酸钠(AES),2.25%十四烷基甲基二羟乙基溴化铵(TMDAB),20%椰油酰胺丙基甜菜碱(CAB-35),0.25%醇醚磺基琥珀酸单酯二钠盐(MES),1.5%椰子油二乙醇酰胺(6501),1%甘油,0.1%二羟甲基海因(DMDMH),少量香精及色素,少量柠檬酸调pH和余量的蒸馏水组成。经过测试表明该洗手液对大肠杆菌和金黄色葡萄球菌的杀菌率达到99.99%和100%,产品稳定性、起泡性好,生产成本较低,与市售的某些抗菌洗手液相比,该洗手液显示出一定的优越性。 By selecting and optimizing the formulation of components,a quaternary ammonium salt based antibacterial hand sanitizer was prepared.The formulation (expressed as mass fraction) consists of 0.75% sodium ethoxylated dodecyl alcohol sulfate (AES),2.25% tetradecylmethyldihydroxyethylammonium bromide (TMDAB),20% cocamidopropyl betaine (CAB-35),0.25% disodium ethoxylated alcohol succinic acid monoester sulfonate (MES),1.5% coconut acid diethanolamide (6501),1% glycerin,0.1% dimethylol dimethyl hydantoin (DMDMH),a little amount of fragrance and colorant,as well as a small amount of citric acid for adjusting pH value.The balance is distilled water.Test results of the product showed that the sterilization efficacy for Escherichia coli and Staphylococcus aureus achieves 99.99% and 100% respectively.The stability and foaming performance of the product are well.Besides,its production cost is rather low.As comparing with some liquid hand cleaners from the market,the prepared product shows somewhat better antibacterial efficacy. 参考文献 | 相关文章 | 计量指标

Select

橙皮素胶束溶液的制备及透明质酸对其稳定性和经皮渗透作用的影响 Preparation of hesperetin micelle pseudo solution and influence of hyaluronic acid on its stability and percutaneous osmosis action 张晓宇, 侯彩平, 赵丽萍, 谢茵, 刘丽清, 何亚丽, 田青平 2016 (2):  92-96.  doi: 10.13218/j.cnki.csdc.2016.02.007 摘要 ( 295 )   PDF(793KB) ( 359 )   制备橙皮素胶束溶液,并以Zeta电位和经皮渗透速率为指标,考察透明质酸对胶束溶液稳定性和经皮渗透活性的影响。结果表明,透明质酸可显著提高橙皮素胶束溶液的稳定性和经皮渗透活性,当其质量分数为0.5%时,胶束溶液透明稳定、无不良气味,室温下pH平均值为5.4±0.1,Zeta电位平均值为-32.62 mV,橙皮素经豚鼠皮肤的稳态渗透速率常数Js达7.475 8 μg/(cm2·h),为对照组的1.5倍。橙皮素胶束溶液耐寒和耐热实验均表现出良好的稳定性。 Pseudo solution of hesperetin micelle was prepared,and influence of hyaluronic acid on its stability and percutaneous osmosis activity was investigated using Zeta potential and percutaneous osmosis rate as the respective indices.Experimental results showed that hyaluronic acid can significantly enhance the stability and percutaneous osmosis activity of hesperetin micelle pseudo solution.When the mass fraction of hyaluronic acid achieves 0.5%,the micelle pseudo solution becomes transparent,stable and without unpleasant odor.Under room temperature condition,its average pH value is 5.4±0.1 and average Zeta potential is -32.62 mV.The steady osmosis rate constant Js of hesperetin through cavy skin achieves 7.475 8 μg/(cm2·h),which is 1.5 multiples as compared with that of the control group.The micelle pseudo solution shows good stability in cold resistance test and heat resistance test. 参考文献 | 相关文章 | 计量指标

Select

酵母提取物对成纤维细胞的作用及抑制黑素生成的研究 Effect of yeast extract on fibroblasts proliferation and melanin inhibition 廖峰,李国光,熊丽丹,杨海延,李利 2016 (2):  97-100.  doi: 10.13218/j.cnki.csdc.2016.02.008 摘要 ( 291 )   PDF(760KB) ( 350 )   通过探索米酒来源酵母提取物(WAE)对成纤维HS68细胞和黑素瘤B16细胞存活率的影响以及对黑素瘤B16细胞黑素生成的作用,研究WAE的安全性及功效性。结果表明,WAE对成纤维HS68细胞和黑素瘤B16细胞的安全质量分数为不高于0.10%;与空白对照组(不含WAE)相比,WAE的质量分数为0.10%和0.01%时,成纤维HS68细胞的存活率分别为106.36%和109.33%,说明WAE在安全质量分数范围内具有促进成纤维HS68细胞增殖的功效;与空白对照组相比,WAE的质量分数为0.10%,0.05%和0.01%时,黑素瘤B16细胞的黑素合成抑制率分别为68.32%,51.68%和19.77%,说明WAE在安全质量分数范围内有抑制黑素瘤B16细胞黑素生成的作用。与相同质量分数的啤酒来源日本酵母提取物(A)和国产酵母提取物(B)相比,WAE的细胞安全性与黑素抑制作用效果更优。 Via the exploration of effect of the yeast extract that originated from rice wine production (WAE) on viability of fibroblast HS68 cells as well as its action for formation of melanin by melanoma B16 cells,the safety and efficacy of the WAE were examined.The results showed that,the safety WAE mass fraction for HS68 and B16 is ≤0.10%.As comparing with the control group,when mass fraction of WAE is 0.10% and 0.01%,it promotes HS68 cells proliferation to 106.36% and 109.33% respectively.The experiments demonstrated that under the safe concentration,WAE displays the effect of promoting proliferation of HS68 cells.WAE can inhibit 68.32%,51.68% and 19.77% of melanin formation when the WAE mass fraction is 0.10%,0.05% and 0.01% respectively.The experiments demonstrated that under the safety mass fraction,WAE displays the effect of inhibiting melanin formation of B16 cells.As comparing with Japanese beer yeast extract (A) and domestic beer yeast extract (B) with the same mass fraction,the safety and efficacy of WAE are superior to that of A and B. 参考文献 | 相关文章 | 计量指标

Select

银耳多糖提取工艺优化及对人体细胞毒性检测 Optimization of extracting Tremella fuciformis polysaccharide and detection of its toxicity to cells of human body 段超,王昌涛 2016 (2):  101-105.  doi: 10.13218/j.cnki.csdc.2016.02.009 摘要 ( 376 )   PDF(2562KB) ( 377 )   以多糖提取率为指标,以料液比(g∶mL)、提取温度和提取时间为影响因素进行热水浸提银耳多糖的单因素试验,在此基础上应用Box-Behnken进行响应面分析。得到较佳工艺条件:料液比为1∶60,提取时间为5.57 h,提取温度为97.18 ℃,理论多糖提取率为20.52%,实际测得值为20.25%,说明该方法可靠。采用MTT(四甲基偶氮唑盐)实验和二氢乙啶(DHE)染色实验检测水提银耳多糖对人皮肤成纤维细胞(HSF)的毒性。MTT实验表明,银耳多糖溶液质量浓度在0~200 mg/L范围内HSF成活率为1,DHE染色实验结果为阴性,说明水提银耳多糖对HSF没有毒性。 Single factor tests for hot water extraction of Tremella fuciformis polysaccharide were conducted with polysaccharide yield as the criteria index as well as solid to solvent ratio (g∶mL),extraction time and extraction temperature as the influencing factors.Based upon the test results,a response surface analysis was derived according to Box-Behnken central composite experimental design.The optimal extraction conditions were obtained as following: solid to solvent ratio,1∶60;extraction time,5.57 h;extraction temperature,97.18 ℃.Under the above conditions,the yield of Tremella fuciformis polysaccharide reaches 20.52% while the yield obtained by quantitative analysis is 20.25%.Such comparison showed that the test method is reliable.Methyl thiazolyl tetrazolium (MTT) test method and dihydroethidium (DHE) staining test method were used for detection of toxicity to fibroblast of human skin (HSF).When the mass concentration of the polysaccharide is within range of 0-200 mg/L,the MTT test showed that the survival rate of HSF achieves 1 and the DHE staining test showed negative.Such results proved that the Tremella fuciformis polysaccharide has no cytotoxicity on HSF. 参考文献 | 相关文章 | 计量指标

Select

结合组织学评分的牛角膜浑浊和渗透性方法研究 Research on bovine corneal opacity and permeability test integrating with histopathological evaluation 陈彧,喻欢,程树军,谈伟君,秦瑶 2016 (2):  106-110.  doi: 10.13218/j.cnki.csdc.2016.02.010 摘要 ( 272 )   PDF(4009KB) ( 397 )   为了优化替代眼刺激动物试验的牛角膜浑浊和渗透性(BCOP)实验的方法,提高其适用范围和预测性,选取新鲜牛眼角膜,直接暴露液体化学受试物作用10 min,后孵育2 h并用荧光素钠处理90 min,采用浊度仪检测角膜浑浊度,用酶标仪检测荧光素钠渗透性,同时将角膜常规固定和制备组织学切片,观察组织学病理变化并评分,基于上述3个指标建立预测模型。结果显示,将角膜浑浊度和渗透性2个定量指标,与角膜上皮组织、基质组织和内皮细胞的组织学变化评分进行组合,可对轻微刺激到中等刺激的范围进行准确细分。研究表明,结合组织学观察的优化BCOP方法可提高其预测性,适用于眼刺激机制研究和化妆品评估。 In order to refine the alternative method for animal eye irritation test——the bovine corneal opacity and permeability (BCOP) test,and to enhance its application scope and predictability,the fresh bovine corneas were chosen and directly exposed to liquid chemical material to be tested for 10 min,post-incubated for 2 h and further treated with fluorescein sodium for 90 min.Then opacity of the treated corneas was measured with opacitometer;the permeability was determined with the multiskan spectrum microplate spectrophotometer and the histopathological changes of the cornea were observed and graded after routinely fixed and paraffin embedding.Finally,the predictive models based on the above three indices were established.The results showed that the corneal opacity and permeability,integrated with histopathological changes of epithelium,stroma and endothelium,the scope of irritation from mild level to moderate level can be accurately distinguished.In conclusion,the refined BCOP method integrated with histological observation could improve its predictability and suitable for study of eye irritation mechanism and cosmetic product evaluation. 参考文献 | 相关文章 | 计量指标

Select

降龙涎二醇的合成工艺研究 Synthesis of ambradiol 郝颜峰, 杨绍祥, 李丽慧, 吕艳羽, 田红玉, 刘永国 2016 (2):  111-113.  doi: 10.13218/j.cnki.csdc.2016.02.011 摘要 ( 468 )   PDF(745KB) ( 366 )   以香紫苏醇为原料,通过KMnO4氧化、LiAlH4还原制备降龙涎二醇。研究了氧化阶段反应物料配比、反应温度、反应时间等因素对降龙涎二醇收率的影响,得出较优反应条件:反应温度为29 ℃,反应时间为5 h,n(香紫苏醇)∶n(KMnO4)=1∶5.6,在此条件下降龙涎二醇的收率为22.3%。 Ambradiol was synthesized by potassium permanganate oxidizing and lithium aluminum hydride reducing from sclareol.Reactant ratio,reaction temperature and reaction time for the oxidation process were studied.The experimental results showed that under conditions as reaction temperature 29 ℃,reaction time 5 h,n(sclareol)∶n(potassium permanganate)=1∶5.6,yield of ambradiol achieves 22.3%. 参考文献 | 相关文章 | 计量指标

专论与综述

Select

抗衰老化妆品体外细胞水平上功效评价方法研究进展 Progress in research work with respect to in vitro evaluation method on cellular level for efficacy of anti-senility cosmetics 郭松鹤,高合意,曾飒,张革 2016 (2):  114-117.  doi: 10.13218/j.cnki.csdc.2016.02.012 摘要 ( 431 )   PDF(764KB) ( 390 )   从角质形成细胞模型抗衰老功效评价方法、成纤维细胞模型抗衰老功效评价方法以及三维皮肤模型抗衰老功效评价方法3方面,综述了抗衰老化妆品体外细胞水平上功效评价方法。在培养的角质形成细胞、成纤维细胞及三维皮肤模型上对化妆品有效成分进行细胞学观察及关键指标分子生物学检测,这些技术对抗衰老产品的功效评价和研发具有广泛的应用价值。 From three aspects for in vitro evaluation method on cellular level for efficacy of anti-senility cosmetics,i.e.the keratinocytes model,the fibroblast model and the three-dimensional skin model were summarized.The evaluation method is mainly based on the cytological observation and the detection of molecular biological indicators in the cell model of keratinocytes,fibroblast and three-dimensional skin.The application of these techniques has a wide range value for the development and evaluation of skin anti-senility products. 参考文献 | 相关文章 | 计量指标

分析与检测

Select

超高效液相色谱法同时测定化妆品中7种美白成分 Simultaneous determination of seven kinds of whitening ingredients in cosmetics by ultra performance liquid chromatography 张斌,钱叶飞,闵春燕,郝刚,陈丽波 2016 (2):  118-122.  doi: 10.13218/j.cnki.csdc.2016.02.013 摘要 ( 260 )   PDF(877KB) ( 409 )   建立了超高效液相色谱-二极管阵列检测器(UPLC-DAD)同时测定化妆品中奥扎格雷、烟酰胺、阿魏酸、苯酚、水杨酸、苯乙基间苯二酚和辛酰水杨酸7种美白成分的方法。样品用50%甲醇水溶液超声提取20 min,采用ACQUITY UPLC HSS T3色谱柱 (2.1 mm×50 mm,1.8 μm),以5 mmol/L的癸烷磺酸钠溶液(含0.1%磷酸)-乙腈为流动相进行梯度洗脱,流速为0.3 mL/min,检测波长为220和277 nm,柱温为30 ℃。结果表明,奥扎格雷、辛酰水杨酸和水杨酸的线性范围分别为0.25~10,0.5~20和2.0~80 mg/L,其他4种化合物的线性范围均为1.0~40 mg/L,相关系数均大于0.999。保留时间和峰面积的日内和日间精密度均较好,相对标准偏差(RSD)均小于2.0%。7种美白成分的方法检出限为0.39~4.33 mg/kg,平均回收率为91.9%~108.2%,RSD为0.1%~2.6%。该方法简单、快速、准确、可靠,适用于化妆品中多种美白成分的同时检测。 A method based on ultra performance liquid chromatography-diode array detector (UPLC-DAD) for simultaneous determination of ozagrel,niacinamide,ferulic acid,phenol,salicylic acid,phenylethyl resorcinol and octanoylsalicylic acid in whitening cosmetics was established.These seven compounds were extracted by 50% methanol aqueous solution under assistance of ultrasonic wave for 20 min.ACQUITY UPLC HSS T3 column (2.1 mm×50 mm,1.8 μm) was used with mobile phase of sodium 1-decanesulfonate with concentration of 5 mmol/L (containing 0.1% phosphoric acid) for conducting gradient elution.The detection wavelength is 220 and 277 nm,and the column temperature is 30 ℃.The linearity ranges in 0.25~10 mg/L for ozagrel,0.5~20 mg/L for octanoylsalicylic acid,2.0~80 mg/L for salicylic acid as well as 1.0~40 mg/L for niacinamide,ferulic acid,phenol and phenylethyl resorcinol.The intra and inter day precision of retention time and peak area are also good.The average recovery rates are from 91.9% to 108.2%,and the relative standard deviations (RSD) are from 0.1% to 2.6%.The method is convenient,accurate,reliable and suitable for the determination of seven compounds above mentioned at the same time. 参考文献 | 相关文章 | 计量指标