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日用化学工业(中英文) ›› 2023, Vol. 53 ›› Issue (4): 398-407.doi: 10.3969/j.issn.1001-1803.2023.04.005

• 开发与应用 • 上一篇    下一篇

莲子心总黄酮多元醇提取工艺筛选及其性能研究

郝晴1,吴二敏1,毕永贤2,刘金俊2,孔德承2,成志伟1,*()   

  1. 1.北京工商大学 化学与材料工程学院 北京市植物资源研究开发重点实验室,北京 100048
    2.浙江宜格企业管理集团有限公司,浙江 杭州 310000
  • 收稿日期:2022-12-09 修回日期:2023-03-23 出版日期:2023-04-22 发布日期:2023-04-25
  • 基金资助:
    北京教委科技面上项目(SQKM201710011010)

Study on screening extraction process of total flavonoids from Nelumbinis Plumula by polyols and its properties

Hao Qing1,Wu Ermin1,Bi Yongxian2,Liu Jinjun2,Kong Decheng2,Cheng Zhiwei1,*()   

  1. 1. Key Laboratory of Plant Resources Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
    2. Zhejiang Yige Enterprise Management Group Co., Ltd, Hangzhou, Zhengjiang 310000, China
  • Received:2022-12-09 Revised:2023-03-23 Online:2023-04-22 Published:2023-04-25
  • Contact: * E-mail: chengzhiwei@btbu.edu.cn.

摘要:

莲子心作为一种药食同源的植物,极具开发潜力。该研究通过单因素实验和响应面Box-Behnken设计实验筛选并优化莲子心总黄酮的多元醇超声辅助提取工艺,对该工艺获得的莲子心提取物进行了安全性及体外抗氧化、抑菌和抗敏活性的测定。结果表明,在提取溶剂为1,2-己二醇,溶剂体积分数87%,料液比3.6:100(g:mL),提取时间39 min的条件下,莲子心总黄酮得率为1.80%±0.08%,且其质量浓度在1.2 g/L以下时无刺激性,对DPPH和ABTS自由基的半数抑制质量浓度分别为0.76和0.69 g/L,对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的最小抑菌质量浓度分别为0.56,1.12和0.14 g/L;且其对透明质酸酶的半数抑制质量浓度为0.35 g/L。该研究提供了一种简单、安全、高效的莲子心总黄酮提取工艺,在保证较高总黄酮得率的基础上兼顾其提取物的生物活性,为莲子心资源的开发提供了理论依据。

关键词: 莲子心, 响应面, 总黄酮, 抗氧化活性, 抑菌活性, 抗敏活性

Abstract:

Nelumbinis Plumula is a kind of medicinal and edible plant with great development potential. Single factor test and response surface test were used to screen and optimize the ultrasonic-assisted extraction process of total flavonoids from Nelumbinis Plumula by polyols, and the safety, in vitro antioxidant, antibacterial and anti-allergy activities of Nelumbinis Plumula extract were determined. Results show that under the conditions of extraction solvent 1,2-hexanediol, solvent volume fraction 87%, material-liquid ratio 3.6:100 (g:mL), and extraction time 39 min, the yield of total flavonoids is 1.80%±0.08%. At 1.2 g/L, the Nelumbinis Plumula extract has no irritation, the half maximal inhibitory concentration of DPPH radical and ABTS radical is 0.76 and 0.69 g/L, respectively, and the minimum inhibitory concentration for Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa are 0.56, 1.12 and 0.14 g/L, respectively. Moreover, the half maximal inhibitory concentration of hyaluronidase is 0.35 g/L. This study provides an easy, safe and efficient method for extracting total flavonoids from Nelumbinis Plumula, which ensures the biological activity of the extract and a high yield of total flavonoids, and provides a theoretical basis for the development of Nelumbinis Plumula.

Key words: Nelumbinis Plumula, response surface, total flavonoids, antioxidant activity, antibacterial activity, anti-allergy activity

中图分类号: 

  • TQ658