黑茶籽油成分分析及其对于H2O2诱导HFF-1细胞氧化损伤的保护作用

doi:10.3969/j.issn.2097-2806.2024.04.004

摘要/Abstract

摘要：

对茶籽油的活性成分及其抗氧化活性进行研究。对不同产地的茶籽油的脂肪酸、α-生育酚含量进行了测定，发现黑茶籽油的三种不饱和脂肪酸（油酸、亚油酸、亚麻酸）总含量（83.72%），α-生育酚（1.33 mg/g）相比于其他茶籽油（绿茶籽油、普洱茶籽油）、山茶籽油（有机山茶油）和油茶籽油（有机油茶籽油、古树茶籽油）含量更高。在此基础上，测试了黑茶籽油对于DPPH自由基、ABTS自由基、羟基自由基的清除能力。结果表明黑茶籽油在0.15 g/mL质量浓度时，对于DPPH、ABTS自由基的清除率分别93.08%和94.85%；在0.3 g/mL质量浓度下，对羟基自由基的清除率为91.25%，相较于其他茶籽油表现出更好的自由基清除效果。此外，还测定了黑茶籽油对于双氧水诱导的人成纤维细胞（HFF-1）氧化应激以及衰老的抑制作用。细胞增殖实验、活性氧自由基测定实验和β-半乳糖苷酶染色实验的结果表明，黑茶籽油在10~50 μg/mL质量浓度下对双氧水诱导的HFF-1细胞氧化应激具有一定的保护作用，表现出抗氧化、抗衰老的活性。

关键词: 黑茶籽油, 自由基, 抗氧化

Abstract:

The active components of tea seed oil and its antioxidant activity were studied. The contents of three unsaturated fatty acids （oleic acid, linoleic acid and linolenic acid） and α-tocopherol in Camellia Sinensis and Camellia Oleifera Abel seed oils from different producing areas, as well as the Camellia Japonica seed oil were analyzed by chromatography. Results show that the total content of three kinds of unsaturated fatty acids is 83.72%, and the content of α-tocopherol is 1.33 mg/g for the Camellia Camellia Sinensis （dark tea） seed oil, which values are higher that of the other kinds of seed oils. Moreover, the scavenging abilities of Camellia Sinensis （dark tea） seed oil for 1,1-diphenyl-2-picrylhydrazyl （DPPH）, 2,2’-azino-bis（3-ethylbenzothiazoline-6-sulfonicacid）（ABTS） and hydroxyl radicals were tested, and the results show that at a mass concentration of 0.15 g/mL, the scavenging rates to DPPH and ABTS radicals are 93.08% and 94.85%, respectively. While the scavenging rate to hydroxyl radicals is 91.25% at a mass concentration of 0.3 g/mL, which shows a better radical scavenging effect than other tea seed oils. In addition, the inhibitory effects of Camellia Sinensis （dark tea） seed oil on hydrogen peroxide induced oxidative stress and senescence of human fibroblasts （HFF-1） were also determined. The results of cell proliferation test, reactive oxygen species （ROS） test and β-galactosidase staining experiment show that Camellia Sinensis （dark tea） seed oil has a certain inhibitory effect on hydrogen peroxide-induced oxidative stress in HFF-1 cells at mass concentrations of 10 to 50 μg/mL, exhibiting antioxidant and anti-aging activities.

Key words: Camellia Sinensis （dark tea） seed oil, free radicals, antioxidant

中图分类号:

TQ658

李健, 邓建明, 黄源城, 余敏, 王光丽, 王靖. 黑茶籽油成分分析及其对于H₂O₂诱导HFF-1细胞氧化损伤的保护作用[J]. 日用化学工业（中英文）, 2024, 54(4): 393-400.

Jian Li, Jianming Deng, Yuancheng Huang, Min Yu, Guangli Wang, Jing Wang. Compositional analysis of dark tea seed oil and its protective effect against H₂O₂-induced oxidative damage in HFF-1 cells[J]. China Surfactant Detergent & Cosmetics, 2024, 54(4): 393-400.

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DPPH		ABTS		·OH
ρ/（g/mL）	清除率/%	ρ/（g/mL）	清除率/%	ρ/（g/mL）	清除率/%
0.05	11.95	0.05	73.38	0.075	35.26
0.08	26.1	0.08	78.68	0.1	52.64
0.1	48.74	0.1	81.2	0.2	74.56
0.125	72.95	0.15	94.85	0.25	82.32
0.15	93.08	0.20	97.65	0.30	91.25

黑茶籽油成分分析及其对于H₂O₂诱导HFF-1细胞氧化损伤的保护作用

Compositional analysis of dark tea seed oil and its protective effect against H₂O₂-induced oxidative damage in HFF-1 cells

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