HPLC-ELSD法测定积雪草中积雪草苷及羟基积雪草苷的含量

doi:10.3969/j.issn.2097-2806.2023.08.015

日用化学工业（中英文） ›› 2023, Vol. 53 ›› Issue (8): 976-980.doi: 10.3969/j.issn.2097-2806.2023.08.015

HPLC-ELSD法测定积雪草中积雪草苷及羟基积雪草苷的含量

王虹丹¹,胥维昌^2,^*(),王远^2,^*(),孔祥文¹,孙丽娟²

1.沈阳化工大学理学院，辽宁沈阳 110000
2.沈阳化工研究院有限公司，辽宁沈阳 110000

收稿日期:2022-08-03 修回日期:2023-07-28 出版日期:2023-08-22 发布日期:2023-08-28
通讯作者: 胥维昌,王远

Determination of asiaticoside and madecassoside in Centella asiatica （L.） Urban by HPLC-ELSD

Wang Hongdan¹,Xu Weichang^2,^*(),Wang Yuan^2,^*(),Kong Xiangwen¹,Sun Lijuan²

1. College of Science, Shenyang University of Chemical Technology, Shenyang, Liaoning 110000, China
2. Shenyang Chemical Research Institute Co., Ltd., Shenyang, Liaoning 110000, China

Received:2022-08-03 Revised:2023-07-28 Online:2023-08-22 Published:2023-08-28
Contact: Weichang Xu,Yuan Wang

摘要/Abstract

摘要：

积雪草提取物通常被用作化妆品原料，有美白、抗氧化等功效，发挥此功效的主要物质有积雪草苷、羟基积雪草苷。采用高效液相色谱-蒸发光散射检测（HPLC-ELSD）技术，建立了测定积雪草中积雪草苷和羟基积雪草苷含量的方法。选用Unitary C₁₈色谱柱（4.6×250 mm，5 μm），以乙腈-水为流动相，梯度洗脱，流速1 mL/min，柱温为30 ℃，采用Agilent 1260 Infinity Ⅱ蒸发光散射检测器（漂移管温度70 ℃，载气流速1.6 L/min）。研究表明，积雪草苷和羟基积雪草苷质量浓度分别在100~702 mg/L，105~738 mg/L范围内，峰面积与质量浓度线性关系良好，相关系数分别为0.999 6和0.999 7。积雪草苷和羟基积雪草苷平均加样回收率分别为101.20%，98.30%。相对标准偏差（RSD）均在3%以内。方法简单，准确度高，分离效果好，干扰少，可用于积雪草中积雪草苷和羟基积雪草苷的含量测定。

关键词: 积雪草, 高效液相色谱-蒸发光散射检测器, 积雪草苷, 羟基积雪草苷

Abstract:

Centella asiatica （L.） Urban extract is usually used as a cosmetic raw material, which has whitening, antioxidant and other effects. The main substances to play this effect are asiaticoside and madecassoside. In this paper, a high performance liquid chromatography-evaporative light scattering detection （HPLC-ELSD） technique was used to determine the content of asiaticoside and madecassoside in Centella asiatica （L.） Urban. Unitary C₁₈column （4.6×250 mm, 5 μm） with acetonitrile-water as the mobile phase was used for gradient elution at a flow rate of 1 mL/min. Column temperature was 30 ℃. Agilent 1260 Infinity Ⅱ detector （drift tube temperature was 70 ℃） was used for column elution with the carrier gas flow rate at 1.6 L/min. The results show that the concentrations of asiaticoside and madecassoside are 100-702 mg/L and 105-738 mg/L, respectively. The linear relationship between peak area and concentration is good, and the correlation coefficients are 0.999 6 and 0.999 7, respectively. The average recoveries of asiaticoside and madecassoside are 101.20% and 98.30%, respectively. The relative standard deviations （RSD） are all within 3%. The method is simple, accurate, effective and less interference, and can be used for the determination of asiaticoside and madecassoside in Centella asiatica （L.） Urban.

Key words: Centella asiatica （L.） Urban, high performance liquid chromatography-evaporative light scattering detector, asiaticoside, madecassoside

中图分类号:

TQ658

王虹丹,胥维昌,王远,孔祥文,孙丽娟. HPLC-ELSD法测定积雪草中积雪草苷及羟基积雪草苷的含量[J]. 日用化学工业（中英文）, 2023, 53(8): 976-980.

Wang Hongdan,Xu Weichang,Wang Yuan,Kong Xiangwen,Sun Lijuan. Determination of asiaticoside and madecassoside in Centella asiatica （L.） Urban by HPLC-ELSD[J]. China Surfactant Detergent & Cosmetics, 2023, 53(8): 976-980.

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参考文献 16

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时间/min	流动相A/%	流动相B/%
0.00	79.00	21.00
22.00	79.00	21.00
30.00	60.00	40.00
30.10	10.00	90.00
35.00	10.00	90.00
35.10	79.00	21.00
40.00	79.00	21.00

标记	取样量/g	样品含量/mg	加入量/mg	测得量/mg	回收率/%	平均回收率/%	RSD/%
80%-1	0.250 3	4.88	3.899 4	8.991 6	105.4	104.9	1.35
80%-2	0.250 1	4.88		8.903 3	103.3
80%-3	0.250 3	4.88		9.014 0	106.0
100%-1	0.250 4	4.88	4.966 7	9.695 7	96.9	98.5	1.49
100%-2	0.250 1	4.88		9.779 5	98.7
100%-3	0.250 3	4.88		9.839 2	99.8
120%-1	0.250 2	4.88	5.922 3	10.911 5	101.8	100.2	1.70
120%-2	0.250 4	4.88		10.819 3	100.2
120%-3	0.250 3	4.88		10.712 1	98.4

标记	取样量/g	样品含量/mg	加入量/mg	测得量/mg	回收率/%	平均回收率/%	RSD/%
80%-1	0.250 1	9.60	7.820 1	17.599 1	102.2	103.1	1.75
80%-2	0.250 2	9.61		17.571 3	101.9
80%-3	0.250 1	9.60		17.832 6	105.2
100%-1	0.250 2	9.61	9.721 3	18.813 9	94.7	96.6	1.97
100%-2	0.250 1	9.60		19.183 0	98.5
100%-3	0.250 4	9.61		19.009 3	96.6
120%-1	0.250 3	9.61	11.623 5	20.984 0	97.9	95.2	2.50
120%-2	0.250 2	9.61		20.456 7	93.4
120%-3	0.250 2	9.61		20.563 2	94.2

样品	含量/%
	“1.2”项色谱条件		药典色谱条件
	积雪草苷	羟基积雪草苷	积雪草苷	羟基积雪草苷
1	2.08	4.01	2.01	3.93
2	1.79	3.72	1.86	3.84
3	1.99	3.80	2.10	3.89
平均值	1.95	3.84	1.99	3.89
方差（S²）	0.011 62	0.022 43	0.009 27	0.002 03

HPLC-ELSD法测定积雪草中积雪草苷及羟基积雪草苷的含量

Determination of asiaticoside and madecassoside in Centella asiatica （L.） Urban by HPLC-ELSD

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