白花泡桐叶片提取物的抗皮肤光老化作用研究

doi:10.3969/j.issn.2097-2806.2024.09.010

摘要/Abstract

摘要：

考察白花泡桐叶片提取物（Paulownia fortunei leaf extract，PFLE）对紫外线B（UVB）诱导的皮肤光老化小鼠的影响。将小鼠分为5组：分别为正常对照组（NC）、模型组（M）、低剂量PFLE组（L-PFLE）、中剂量PFLE组（M-PFLE）、高剂量PFLE组（H-PFLE）。NC组小鼠不照射。其他组小鼠使用紫外线光疗仪进行UVB照射。UVB照射当天，NC组和M组小鼠背部涂抹雪花膏，L-PFLE组、M-PFLE组和H-PFLE组小鼠分别涂抹50，100和200 mg/kg的PFLE乳膏。UVB照射和给药周期均为6周。检测了小鼠的体重、皮肤含水量、皮肤氧化应激指标、HYP和HA水平。采用HE染色观察小鼠皮肤形态。通过Western blotting检测了小鼠皮肤中Nrf2（细胞核）、Keap1和HO-1的蛋白表达水平。通过RT-qPCR检测了小鼠皮肤中MMP-1、MMP-3的转录水平。结果显示，与M组比较，L-PFLE组、M-PFLE组和H-PFLE组小鼠皮肤含水量升高，皮肤形态明显改善，SOD、CAT和GSH-Px水平升高，MDA水平降低，Nrf2（细胞核）和HO-1蛋白相对表达量升高，Keap1蛋白相对表达量降低，HYP和HA水平升高，MMP-1和MMP-3 mRNA相对表达量降低（P<0.05）。表明PELE经皮给药具有良好的抗皮肤光老化作用。

关键词: 白花泡桐叶片提取物, 皮肤光老化, 抗氧化, 羟脯氨酸, 透明质酸

Abstract:

The study aimed to investigate the effect of Paulownia fortunei leaf extract （PFLE） on ultraviolet B （UVB）-induced skin photoaging in mice. The mice were divided into 5 groups: normal control group （NC）, model group （M）, low-dose PFLE group （L-PFLE）, medium-dose PFLE group （M-PFLE）, and high-dose PFLE group （H-PFLE）. Mice in NC group were not irradiated. Mice in the other groups were irradiated with UVB using ultraviolet photo-therapy equipment. On the day of UVB irradiation, mice in NC group and M group were smeared with cream on their backs, and mice in L-PFLE, M-PFLE and H-PFLE groups were smeared with 50, 100 and 200 mg/kg PFLE cream, respectively. The UVB irradiation and administration cycles were both 6 weeks. The body weight, skin moisture content, skin oxidative stress indicators （SOD, CAT, GSH-Px and MDA）, HYP and HA levels of the mice were detected. HE staining was used to observe skin morphology. The protein expression levels of Nrf2 （nucleus）, Keap1 and HO-1 in the skin were detected by Western blotting. The transcript levels of MMP-1 and MMP-3 in the skin were detected by RT-qPCR. The results show that compared with M group, the skin moisture content of the mice in L-PFLE group, M-PFLE group and H-PFLE group increases, the skin morphological changes are significantly improved, the levels of SOD, CAT and GSH-Px increase, the MDA level decreases, the relative expressions of Nrf2 （nucleus） and HO-1 protein increase, the relative expression of Keap1 protein decreases, the levels of HYP and HA increase, and the relative expressions of MMP-1 and MMP-3 mRNA decrease （P<0.05）. This study shows that transdermal administration of PELE has a good anti-skin photoaging effect.

Key words: Paulownia fortunei leaf extract, skin photoaging, antioxidant, hydroxyproline, hyaluronic acid

中图分类号:

TQ658

孙琳, 张曼. 白花泡桐叶片提取物的抗皮肤光老化作用研究[J]. 日用化学工业（中英文）, 2024, 54(9): 1092-1098.

Lin Sun, Man Zhang. Study on the anti-photoaging effect of Paulownia fortunei leaf extract[J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1092-1098.

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