洋甘菊总黄酮提取及其抗氧化活性研究

doi:10.3969/j.issn.2097-2806.2024.09.004

摘要/Abstract

摘要：

通过优化洋甘菊总黄酮的提取和分离纯化工艺，研究了先用超临界CO₂去除洋甘菊脂溶性成分，再结合超声辅助提取洋甘菊总黄酮，用大孔树脂对洋甘菊总黄酮进行分离纯化，并对洋甘菊总黄酮成分进行了分析及表征。得到超临界CO₂去除洋甘菊脂溶性成分的较佳工艺条件为：萃取温度50 ℃、萃取时间60 min、萃取压力25 MPa，洋甘菊除脂率为8.66%；超声辅助提取洋甘菊总黄酮的较佳工艺条件为：超声时间15 min、乙醇体积分数50%、料液比1∶25 （g∶mL）、超声功率200 W，总黄酮提取率为4.25%；选用AB-8树脂，纯化后洋甘菊总黄酮的纯度由6.04%提高到52.25%，而不结合超临界CO₂萃取法的单一超声辅助提取法采用同样纯化工艺后洋甘菊总黄酮的纯度仅为25.47%。结果表明超临界CO₂萃取法和超声辅助提取法的结合工艺能更加高效提取洋甘菊总黄酮，并能降低洋甘菊总黄酮的气味，纯化后的洋甘菊总黄酮可较好的清除DPPH及ABTS自由基，具有较强的抗氧化活性。

关键词: 洋甘菊, 总黄酮, 提取, 纯化, 抗氧化活性

Abstract:

By optimizing the extraction and purification process, the study first used supercritical CO₂ to remove the lipophilic components of chamomile, and then combined with ultrasound assisted the extraction of total flavonoids from chamomile. The total flavonoids in chamomile were separated and purified using macroporous resin, and the total flavonoid components were analyzed and characterized. The optimal process conditions for obtaining supercritical CO₂ to remove fat soluble components from chamomile are: extraction temperature of 50 ℃, extraction time of 60 minutes, extraction pressure of 25 MPa, and chamomile fat removal rate of 8.66%. The optimal process conditions for ultrasound assisted extraction of total flavonoids from chamomile are: ultrasound time of 15 minutes, ethanol volume fraction of 50%, solid-liquid ratio of 1∶25 （g∶mL）, ultrasound power of 200 W, and total flavonoid extraction rate of 4.25%. After purification, the purity of total flavonoids in chamomile increase from 6.04% to 52.25%, while the purity of total flavonoids in chamomile is only 25.47% using the same purification process using a single ultrasound assisted extraction method without supercritical CO₂ extraction. The results show that the combination of supercritical CO₂ extraction and ultrasound assisted extraction can more efficiently extract total flavonoids from chamomile, and reduce the odor. The purified total flavonoids from chamomile can effectively scavenge DPPH and ABTS free radicals, and have strong antioxidant activity.

Key words: chamomile, total flavonoids, extract, purification, antioxidant activity

中图分类号:

TQ658

李传茂, 邱志, 王枫亮, 林盛杰, 邓慧, 李映伟. 洋甘菊总黄酮提取及其抗氧化活性研究[J]. 日用化学工业（中英文）, 2024, 54(9): 1039-1049.

Chuanmao Li, Zhi Qiu, Fengliang Wang, Shengjie Lin, Hui Deng, Yingwei Li. Extraction and antioxidant activity of total flavonoids from chamomile[J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1039-1049.

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树脂类型	性质	比表面积/（m²/g）	平均孔径/nm
DM-130	弱极性	500~550	9~10
AB-8	弱极性	480~520	13~14
D-101	非极性	550~600	9~11
NKA	非极性	570~590	20~22
NKA-9	极性	250~290	15.5~16.5

实验号	A	B	C	除脂率/%
1	1	1	1	7.104 00
2	1	2	2	7.810 00
3	1	3	3	7.540 00
4	2	1	2	8.445 45
5	2	2	3	8.414 20
6	2	3	1	8.498 48
7	3	1	3	7.681 54
8	3	2	1	7.931 00
9	3	3	2	8.390 00
K1	22.454 00	23.230 99	23.533 48
K2	25.358 13	24.155 2	24.645 45
K3	24.002 54	24.428 48	23.635 74
k1	7.484 67	7.743 66	7.844 49
k2	8.452 71	8.051 73	8.215 15
k3	8.000 85	8.142 83	7.878 58
R	0.516 18	0.308 07	0.336 57

树脂型号	吸附量/（mg/g）	吸附率/%	解吸率/%
DM-130	17.61	71.47	82.22
AB-8	20.89	84.78	86.94
D-101	19.79	80.31	78.42
NKA	19.35	78.53	80.18
NKA-9	16.86	68.42	87.91