经皮应用水飞蓟提取物的抗衰老作用评价

doi:10.3969/j.issn.2097-2806.2024.08.013

摘要/Abstract

摘要：

经皮应用水飞蓟提取物（Silybum marianum extract，SME），研究SME对D-半乳糖（D-galactose，D-gal）诱导的皮肤衰老小鼠的抗衰老作用。将小鼠分为6组，连续6周背部皮下注射D-gal （1 g/ （kg·d））诱导皮肤衰老小鼠模型。检测各组小鼠的体重、皮肤含水量、皮肤氧化应激指标、HYP和HA水平。采用HE染色评价了小鼠皮肤损伤。采用RT-qPCR检测了小鼠皮肤中collagen Ⅰ、collagen Ⅲ、MMP-1和MMP-3的mRNA相对表达量。采用Western blotting检测了小鼠皮肤中Nrf2（细胞核）、Keap1和HO-1的蛋白相对表达量。结果显示，经皮应用3个剂量的SME均升高了D-gal诱导的皮肤衰老小鼠的皮肤含水量，减轻了皮肤损伤，升高了SOD、CAT和GSH-Px水平，降低了MDA水平，升高了HYP和HA水平，升高了collagen Ⅰ和collagen Ⅲ mRNA相对表达量，降低了MMP-1和MMP-3 mRNA相对表达量，升高了Nrf2（细胞核）和HO-1蛋白相对表达量，降低了Keap1蛋白相对表达量（P<0.05）。抑制剂ML385抑制了Nrf2途径的激活并减弱了SME的抗衰老作用（P<0.05）。本研究表明，经皮应用SME对D-gal诱导的皮肤衰老小鼠具有良好的抗衰老作用，Nrf2途径介导了SME的抗衰老作用。综上，SME有望成为一种具有较高开发价值的抗衰老天然产物。

关键词: 水飞蓟提取物, 经皮应用, 抗衰老, 氧化应激

Abstract:

The study aims to investigate the anti-aging effect of Silybum marianum extract （SME） on skin aging mice induced by D-galactose （D-gal）. The mice were divided into 6 groups. The mouse model of skin aging was induced by subcutaneous injection of D-gal （1 g/ （kg·d）） on the back for 6 weeks. Body weight, skin water content, skin oxidative stress index, HYP and HA levels of mice in each group were measured. HE staining was used to evaluate the skin injury. The relative mRNA expressions of collagen Ⅰ, collagen Ⅲ, MMP-1 and MMP-3 in skin were detected by RT-qPCR. The relative expressions of Nrf2 （nucleus）, Keap1 and HO-1 in skin were detected by Western blotting. The results show that percutaneous application of all three doses of SME increase the skin water content, alleviate the skin injury, increase the levels of SOD, CAT and GSH-Px, decrease the level of MDA, increased the levels of HYP and HA, increase the relative expression of collagen Ⅰ and collagen Ⅲ mRNA, decrease the relative expression of MMP-1 and MMP-3 mRNA, increase the relative expression of Nrf2 （nucleus） and HO-1 protein, and decrease the relative expression of Keap1 protein in D-gal-induced aging mice （P<0.05）. ML385 inhibits the activation of Nrf2 pathway and weakens the anti-aging effect of SME （P<0.05）. This study shows that percutaneous application of SME has a good anti-aging effect on skin aging mice induced by D-gal, and Nrf2 pathway mediates the anti-aging effect of SME. SME may be a kind of anti-aging daily skin care material with high development value.

Key words: Silybum marianum extract, percutaneous application, anti-aging, oxidative stress

中图分类号:

TQ658

何美玲,范丽敏. 经皮应用水飞蓟提取物的抗衰老作用评价[J]. 日用化学工业（中英文）, 2024, 54(8): 981-987.

Meiling He,Limin Fan. Evaluation of anti-aging effect of percutaneous application of Silybum marianum extract[J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 981-987.

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Gene	Primer sequence
collagen Ⅰ	F：5′-AAACGTAGTCGATGCTGATGC-3′
collagen Ⅰ	R：5′-AGCTGATGCTGATCGTAGTCGTA-3′
collagen Ⅲ	F：5′-ACGTAGTCGTAAGGTCGTGA-3′
collagen Ⅲ	R：5′-CTGATGCTGATGCTGATCGTAA-3′
MMP-1	F：5′-ATAGATTCATGCCAGAACCTGA-3′
MMP-1	R：5′-TGCCTTTGAAATAGCGGACT-3′
MMP-3	F：5′-AATCAGTTCTGGGCTATACGA-3′
MMP-3	R：5′-TCGATCTTCTTCACGGTTGC-3′
GAPDH	F：5′-CCTCGTCCCGTAGACAAAATG-3′
GAPDH	R：5′-TGAGGTCAATGAAGGGGTCGT-3′