驴乳延缓皮肤衰老的研究

doi:10.3969/j.issn.1001-1803.2021.08.009

摘要/Abstract

摘要：

使用驴乳溶液处理正常人原代真皮成纤维细胞（HDFn细胞）和人永生化表皮细胞（HaCaT细胞）,检测了驴乳对细胞活性、细胞增殖、损伤修复的影响。研究了驴乳溶液处理HDFn细胞后细胞外基质（胶原蛋白、基质金属蛋白酶）含量的影响。质量浓度为0.63~10 mg/mL驴乳作用于HDFn细胞和HaCaT细胞,细胞安全无毒性。通过BrdU法检测不同质量浓度的驴乳对HDFn细胞和HaCaT细胞的增殖能力,5、10 mg/mL驴乳具有显著的促进细胞增殖的作用（p<0.01）。与阴性对照相比,5、10 mg/mL驴乳可促进HDFn细胞中ColⅠ合成（p<0.01）,抑制MMP-1分泌。5、10 mg/mL 驴乳作用HDFn细胞24 h后,与阴性对照相比,细胞愈合率显著提高（p<0.01）。驴乳可促进细胞增殖、增加胶原蛋白含量、抑制MMP-1分泌、促进损伤修复,发挥延缓皮肤衰老的作用,为其在护肤品中的应用提供科学依据。

关键词: 驴乳, 抗衰老, 细胞增殖, 胶原蛋白, 基质金属蛋白酶, 划痕试验

Abstract:

The anti-aging effect of donkey milk on skin was evaluated. More and more attention has been paid to donkey milk in recent years since its nutrition composition shows higher similarity to human milk than others. Donkey milk has not only high nutritional value but also a wide range of medicinal value. Herein, the measurement of cell activity, cell proliferation, extracellular matrix （collagen, matrix metalloproteinase） content and invitro wound healing by the scratch assay were used. Donkey milk was found to be safe and nontoxic up to the concentration of 0.63- 10 mg/mL. Bromodeoxyuridine （BrdU） assay data showed the proliferation of HDFn cells and HaCaT cells after 48 h, and it was found that when the concentration of donkey milk was 5 and 10 mg/mL, the proliferation of cells was increased significantly （p<0.01）. The collagen in extracellular matrix was increased by 38% at 10 mg/mL donkey milk compared to the control group （p<0.01）. The content of MMP-1 secreted by HDFn cells induced by UVB was significantly reduced after treated with 5 and 10 mg/mL donkey milk. In addition, wound healing assay exhibited significant wound closure and cell migration compared to the control group after HDFn cells being treated with donkey milk for 24 h. In conclusion, donkey milk can promote cell proliferation, increase collagen content, inhibit MMP-1 secretion, promote damage repair, and thereby delay skin aging. It provides a necessary scientific basis for the application of donkey milk in functional cosmetics; however, the roles and specific molecular mechanism in delaying skin aging need to be further explored.

Key words: donkey milk, anti-aging, cell proliferation, collagen, matrix metalloproteinase, scratch assay

中图分类号:

TQ658

刘春媛,樊雨梅,何婕,周广运,苏宁,廖峰. 驴乳延缓皮肤衰老的研究[J]. 日用化学工业, 2021, 51(8): 754-760.

Liu Chunyuan,Fan Yumei,He Jie,Zhou Guangyun,Su Ning,Liao Feng. Study on the effects of donkey milk in delaying skin aging[J]. China Surfactant Detergent & Cosmetics, 2021, 51(8): 754-760.

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