小球藻发酵产物抗衰老功效研究及安全性评价

doi:10.3969/j.issn.1001-1803.2022.05.011

摘要/Abstract

摘要：

以小球藻为底物材料,利用植物乳杆菌,发酵制备了小球藻发酵产物化妆品原料。以小球藻发酵产物为研究对象,通过人成纤维细胞增殖试验、Ⅰ型胶原蛋白含量检测试验以及ABTS自由基清除试验等,对其体外抗衰老功效进行了研究,并通过人体封闭式斑贴试验对其安全性进行评价。结果显示,体积分数为10%的小球藻发酵产物能够提高人皮肤成纤维细胞的存活率,可达到185.71%±2.25%（P<0.01）,同时能够提高该细胞合成Ⅰ型胶原蛋白的能力,是阴性对照的（4.43±0.34）倍（P<0.01）;小球藻发酵产物能有效清除ABTS自由基,且呈剂量依赖性模型,当体积分数达到40.5%时,对ABTS自由基的清除率达到62.73%±1.4%（P<0.01）;人体封闭式斑贴试验结果显示小球藻发酵产物具有较好的皮肤安全性。实验表明,小球藻发酵产物具有较好的抗衰老功效且对人体具有较好的安全性。

关键词: 小球藻, 发酵, 化妆品原料, 抗衰老, 安全性

Abstract:

Chlorella is a kind of single-celled green algae, which is rich in high-quality protein, linolenic acid, polysaccharides, nucleotides, peptides, vitamins, trace elements and other substances, showing anti-virus, anti-cancer, anti-oxidation, anti-inflammatory functions, etc. Its nutrient composition and biological activity are becoming a research hotspot in recent years. Herein, Chlorella was used as a substrate material to produce Chlorella ferment for cosmetics by fermentation with Lactobacillus plantarum. Taking the Chlorella fermentation product as the research object, the study on its anti-aging effect in vitro and evaluation of its safety were carried out through a variety of methods such as human fibroblast proliferation test, type Ⅰ collagen content test （ELISA）, ABTS free radical scavenging test and the human body closed patch test. The results showed that the Chlorella fermentation product with a volume fraction of 10% could increase the survival rate of human skin fibroblasts, reaching 185.71%±2.25% （P<0.01）, and meanwhile the ability of human skin fibroblasts to synthesize type I collagen was improved, which was 4.43±0.34 times of the negative control （P<0.01）. The Chlorella fermentation product could effectively scavenge ABTS free radicals in a dose-dependent model. When the volume fraction reached 40.5%, the scavenging rate against ABTS free radicals reached 62.73%±1.4% （P<0.01）. The results of the human closed patch test showed that the Chlorella fermentation product had good skin safety. In summary, the Chlorella fermentation product can promote the proliferation of human fibroblasts, increase the content of type I collagen, and scavenge ABTS free radicals, thereby exhibiting good anti-aging effects and good safety to the human body. This provides a better basis for the development and utilization of Chlorella in the field of cosmetics.

Key words: Chlorella, fermentation, raw material of cosmetics, anti-aging, safety

中图分类号:

TQ658

李海军,马双双,郑德强,张英华,王庆波,薛泽. 小球藻发酵产物抗衰老功效研究及安全性评价[J]. 日用化学工业, 2022, 52(5): 534-538.

Li Haijun,Ma Shuangshuang,Zheng Deqiang,Zhang Yinghua,Wang Qingbo,Xue Ze. Study on the anti-aging efficacy and safety evaluation of Chlorella fermentation product[J]. China Surfactant Detergent & Cosmetics, 2022, 52(5): 534-538.

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反应程度	评分等级	皮肤反应
-	0	阴性反应
±	1	可疑反应,仅有微弱红斑
+	2	弱阳性反应（红斑反应）：红斑、浸润、水肿、可有丘疹
++	3	强阳性反应（疱疹反应）：红斑、浸润、水肿、丘疹、疱疹;反应可超出受试区
+++	4	极强阳性反应（融合性疱疹反应）：明显红斑、严重浸润、水肿、融合性疱疹;反应超出受试区

体积分数/%	细胞存活率/%
阴性对照	100.00±3.18
0.08	99.98±3.31
0.16	102.72±4.26
0.32	103.19±4.97
0.63	105.64±2.35*
1.25	108.78±3.96*
2.50	120.03±6.15**
5.00	147.77±5.31**
10.00	185.71±2.25**

样品体积分数/%	Ⅰ型胶原蛋白相对含量
阴性对照	1.00±0.19
阳性对照	2.90±0.05
10	4.43±0.34**

样品体积分数/%	ABTS自由基清除率/%
阴性对照	0.00±2.17
0.5	0.49±1.11
1.5	3.14±0.73
4.5	13.97±0.33**
13.5	36.56±1.99**
40.5	62.73±1.40**

样品名称	反应程度	反应例数（去除斑试器）			反应人数
样品名称	反应程度	30 min后	24 h后	48 h后	反应人数
空白	-	30	30	30	0
	±	0	0	0
	+	0	0	0
	++	0	0	0
	+++	0	0	0
小球藻发酵产物	-	1	29	29	1
	±	0	0	0
	+	0	0	0
	++	0	0	0
	+++	0	0	0