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日用化学工业（中英文）

日用化学工业 ›› 2021, Vol. 51 ›› Issue (4): 324-330.doi: 10.3969/j.issn.1001-1803.2021.04.010

• 开发与应用 • 上一篇    下一篇

双向发酵提取燕麦β-葡聚糖的护肤功效研究

付豪1(),吴迪1,邴雪1,张佳婵1,2,王昌涛1,2,李萌1,2()   

  1. 1.北京工商大学 化学与材料工程学院 北京市植物资源研究开发重点实验室,北京 100048
    2.北京工商大学 北京食品营养与人类健康高精尖创新中心,北京 100048
  • 收稿日期:2020-07-21 修回日期:2021-03-30 出版日期:2021-04-22 发布日期:2021-04-27
  • 通讯作者: 李萌
  • 作者简介:付 豪（1997-）,男,河北人,硕士研究生,电话：18811359600,E-mail： 18811359600@163.com。

Study on the skin care effect of oat β-glucan extracted by bidirectional fermentation

FU Hao1(),WU Di1,BING Xue1,ZHANG Jia-chan1,2,WANG Chang-tao1,2,LI Meng1,2()   

  1. 1. Beijing Key Lab of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100048, China
    2. Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology and Business University, Beijing 100048, China
  • Received:2020-07-21 Revised:2021-03-30 Online:2021-04-22 Published:2021-04-27
  • Contact: Meng LI

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摘要：

以燕麦作为培养基,通过双向发酵法提取燕麦中的β-葡聚糖,进行化妆品护肤功效研究。选用黄伞、大杯伞和灰树花3种真菌与燕麦进行双向发酵,实验结果表明,燕麦发酵后细胞毒性明显的降低。通过对成纤维细胞内活性氧（ROS）含量、Ⅰ型胶原蛋白（Col Ⅰ）含量及基质金属蛋白酶（MMP-1）含量进行测定发现,与发酵前相比,发酵液及纯化燕麦β-葡聚糖所作用的成纤维细胞内活性氧含量相对降低,Col I含量相对升高,MMP-1含量相对降低,证明发酵液具有一定的抗衰老功效,且比未经发酵处理的燕麦效果要好。通过对小鼠黑素瘤细胞（B16细胞）内黑色素含量及酪氨酸酶激活率的测定发现,发酵液处理后的细胞内黑色素含量更低,其中黄伞燕麦发酵液的效果最好。Franz扩散池透皮吸收实验证明发酵液的累积透过量更高。

关键词: 双向发酵, 燕麦β-葡聚糖, 药用真菌, 抗衰老, 美白, 透皮吸收

Abstract:

Using oats as a culture medium, the β-glucan in oats was extracted by a bidirectional fermentation method, and its efficacy of cosmetic skin care was studied. Three fungi, i.e., Pholiota adiposa, Clitocybe maxima and Grifola frondose, were used for bidirectional fermentation with oats. The experimental results showed that the cytotoxicity of oats is significantly reduced after fermentation. By measuring the content of reactive oxygen species （ROS）, type I collagen （Col I） and matrix metalloproteinase （MMP-1） in fibroblasts, it was found that the fermentation broth had certain anti-aging effect, and the effect was better than that of unfermented oats. Compared with those before fermentation, the fermentation broth and purified fermented oat β-glucan showed relatively reduced content of active oxygen, relatively high content of Col I, and relatively low content of MMP-1in fibroblasts. By measuring the melanin content and tyrosinase activation rate in mouse melanoma cells（B16 cells）, it was found that the melanin content in the cells after being treated with the fermentation broth was lower, and the Pholiota adiposa/oat fermentation broth had the best effect. Franz diffusion cell transdermal absorption experiments had showed that the cumulative permeation amount of the fermentation broth is higher.

Key words: bidirectional fermentation, oat β-glucan, medicinal fungi, anti-aging, whitening, transdermal absorption

中图分类号: 

  • TQ658