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日用化学工业（中英文）

日用化学工业 ›› 2022, Vol. 52 ›› Issue (2): 166-171.doi: 10.3969/j.issn.1001-1803.2022.02.009

• 开发与应用 • 上一篇    下一篇

珍珠灵芝复配物美白功效与机理初步研究

金佳颖1,2,陈露1,2,王欣之1,2,3,刘睿1,2,3,吴皓1,2,3,*()   

  1. 1. 南京中医药大学 药学院,江苏 南京 210023
    2. 江苏省海洋药用生物资源研究与开发重点实验室,江苏 南京 210023
    3. 江苏省中药资源产业化过程协同创新中心,江苏 南京 210023
  • 收稿日期:2021-04-19 修回日期:2022-01-06 出版日期:2022-02-25 发布日期:2022-02-22
  • 通讯作者: 吴皓
  • 基金资助:
    药用南珠标准化养殖与综合利用产业链协同创新项目(BHsfs002);2020年江苏省研究生实践创新计划项目(SJCX20_0551)

Whitening efficacy and mechanism study of compound of Ganoderma atrum （mushroom） extract and pearl extract

Jin Jiaying1,2,Chen Lu1,2,Wang Xinzhi1,2,3,Liu Rui1,2,3,Wu Hao1,2,3,*()   

  1. 1. College of Pharmacology, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China
    2. Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing, Jiangsu 210023, China
    3. Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing, Jiangsu 210023, China
  • Received:2021-04-19 Revised:2022-01-06 Online:2022-02-25 Published:2022-02-22
  • Contact: Hao Wu

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摘要：

为研究珍珠灵芝提取物复配在美白类化妆品中开发的优势,采用体外酪氨酸酶活性和小鼠B16黑色素瘤细胞模型实验,分别对黑灵芝提取物、珍珠提取物及其复配物的美白功效进行评价,测定三者对体外酪氨酸酶活性、B16细胞相对黑色素含量、酪氨酸酶（TYR）和酪氨酸酶相关蛋白-1（TRP1）mRNA表达水平的影响。结果表明,1%黑灵芝提取物、2%珍珠提取物及二者组成的复配物均可显著抑制酪氨酸酶活性（P<0.05）、降低细胞内黑色素含量（P<0.05）,其中复配物组对酪氨酸酶活性、细胞内黑色素生成抑制率分别达32.87%、68.27%,均优于单用组（P<0.05）;此外,复配物组可显著下调TRP1 mRNA表达水平（P<0.05）,而单用组无显著下调TRP1 mRNA表达水平的效果,提示复配物组较单用组有更好地抑制真黑色素生成的功效,具有开发成为化妆品功效添加剂加入美白化妆品的潜力。

关键词: 珍珠提取物, 美白, 黑色素, B16细胞

Abstract:

The literature reported that both pearl and Ganoderma lucidum had whitening effects, and both of them had products in cosmetics field, but the compound of pearl and Ganoderma lucidum was seldom reported. The purpose of this article was to study the advantages of the compound of pearl extract and Ganoderma lucidum extract in the development of whitening cosmetics. The whitening effects of Ganoderma atrum （mushroom） extract, pearl extract and their compound extracts were evaluated by in vitro tyrosinase and B16 cell model. The effects on tyrosinase activity in vitro, B16 cell viability, relative melanin content in B16 cells, expression level of tyrosinase （TYR） and tyrosinase-related protein 1（TRP1） mRNA were studied. The results of B16 cell viability show that Ganoderma atrum （mushroom） extract, pearl extract and their compound extracts have no cytotoxicity, and they can be used to B16 cells to next step. The results of tyrosinase activity and relative melanin content in B16 cells show that 1% Ganoderma atrum （mushroom） extract, 2% pearl extract and the compound extract can significantly inhibit tyrosinase activity （P<0.05） and reduce melanin content in B16 cells（P<0.05）. Inhibition rate of tyrosinase activity of the compound extract reaches 32.87%, and inhibition rate of melanin synthesis of the compound extract reaches 68.27%. Compared the compound extract with Ganoderma atrum （mushroom） extract and pearl extract, the compound extract has better effect of reducing the melanin content in B16 cells （P<0.05）. The results of the mRNA expression levels of tyrosinase （TYR） and tyrosinase-related protein 1（TRP1） show that the group of the compound extract can significantly reduce mRNA expression levels of TYR and TRP1 （P<0.05）, while single groups can only significantly reduce TYR mRNA expression levels. TRP1 can regulate the production of eumelanin, which is a species of melanin. The results show that the compound extract has better effect on inhibiting the formation of eumelanin, indicating that 2% pearl extract combined with 1% Ganoderma atrum （mushroom） extract have better effect on inhibiting the formation of melanin and it is related with its significant reduction of TRP1 mRNA expression. The compound extract has potential to develop as a cosmetic efficacy additive into whitening cosmetics.

Key words: pearl extract, whitening, melanin, B16 cell

中图分类号: 

  • TQ658