芝麻林素对皮肤光老化小鼠AQP3表达和Nrf2信号通路活化的影响

doi:10.3969/j.issn.2097-2806.2024.04.009

摘要/Abstract

摘要：

研究芝麻林素（Sesamolin，SES）对紫外线B（UVB）诱导的皮肤光老化小鼠的影响。将小鼠随机分为5组（n=12）：Control组、UVB组、低剂量SES组（L-SES）、中剂量SES组（M-SES）和高剂量SES组（H-SES）。其中Control组小鼠不进行UVB照射，其他组小鼠均进行8周UVB照射。此外，照射当天开始，L-SES组、M-SES组和H-SES组小鼠分别灌胃0.5 mL 20，40和80 mg/（kg·d）的芝麻林素溶液，共给药8周。给药结束后，分别测定各组小鼠的表皮含水量。通过HE染色评价皮肤组织形态，通过Masson三色染色评价胶原蛋白沉积。测定皮肤组织中氧化应激指标（SOD、CAT、GSH-Px和MDA）水平。通过RT-qPCR和Western blotting检测皮肤组织中MMP-1、Collagen I、Nrf2、Keap1、HO-1、AQP3的mRNA或蛋白水平。结果显示，与UVB组比较，L-SES组、M-SES组和H-SES组小鼠表皮含水量升高，表皮厚度降低，皮肤病变减轻，胶原纤维排列整齐，胶原密度升高，MMP-1 mRNA和蛋白表达水平降低，Collagen I mRNA和蛋白表达水平升高，SOD、CAT和GSH-Px水平升高，MDA水平降低，Nrf2和HO-1的mRNA和蛋白相对表达量升高，Keap1 mRNA和蛋白相对表达量降低，AQP3的mRNA和蛋白相对表达量升高（P<0.05）。表明芝麻林素可剂量依赖性的提高小鼠表皮含水量和抗氧化能力，减轻皮肤损伤，其机制可能与上调AQP3的表达和激活Nrf2信号通路有关。

关键词: 芝麻林素, 紫外线B, 皮肤光老化, 皮肤含水量

Abstract:

The study aimed to reveal the effect of Sesamolin （SES） on skin photoaging mice induced by ultraviolet B （UVB）. Mice were randomly divided into five groups （n=12）: control group, UVB group, low dose SES group （L-SES）, medium dose SES group （M-SES） and high dose SES group （H-SES）. Mice in control group were not irradiated with UVB, while mice in other groups were irradiated with UVB for 8 weeks. In addition, from the day of irradiation, mice in L-SES group, M-SES group and H-SES group were administrated with 0.5 mL Sesamolin solution of 20, 40 and 80 mg/（kg·d）, respectively, for a total of 8 weeks. After administration, the water content of epidermis of mice in each group was measured. Skin morphology was evaluated by HE staining and collagen deposition was evaluated by Masson trichrome staining. The levels of oxidative stress indexes （SOD, CAT, GSH-Px and MDA） in skin tissue were measured. The mRNA or protein levels of MMP-1, Collagen I, Nrf2, Keap1, HO-1 and AQP3 in skin tissue were detected by RT-qPCR and Western blotting. Compared with UVB group, the water content of epidermis in L-SES group, M-SES group and H-SES group increases, the thickness of epidermis decreases, the skin lesion alleviates, the collagen fibers arrange neatly, the collagen density increases, the expression levels of MMP-1 mRNA and protein decrease, the expression levels of Collagen I mRNA and protein increase, the levels of SOD, CAT and GSH-Px increase, the level of MDA decreases, and the relative expression levels of mRNA and protein in Nrf2 and HO-1 increase, the relative expression levels of Keap1 mRNA and protein decrease, while the relative expression levels of mRNA and protein of AQP3 increase （P<0.05）. Sesamolin can increase the water content and antioxidant capacity of mouse epidermis in a dose-dependent manner and reduce skin injury. The mechanism may be related to the up-regulation of AQP3 expression and activation of Nrf2 signal pathway.

Key words: sesamolin, ultraviolet B, skin photoaging, skin water content

中图分类号:

TQ658

韩耕涛, 董卓, 姚韧辉. 芝麻林素对皮肤光老化小鼠AQP3表达和Nrf2信号通路活化的影响[J]. 日用化学工业（中英文）, 2024, 54(4): 431-438.

Gengtao Han, Zhuo Dong, Renhui Yao. Effects of sesamolin on AQP3 expression and Nrf2 signaling pathway activation in skin photoaging mice[J]. China Surfactant Detergent & Cosmetics, 2024, 54(4): 431-438.

图/表 10

图1

图2

图3

图4

表1

图5

图6

图7

图8

图9

参考文献 22

[1]	Afnan Q, Kaiser P J, Rafiq R A, et al. Glycyrrhizic acid prevents ultraviolet-B-induced photodamage: a role for mitogen-activated protein kinases, nuclear factor kappa B and mitochondrial apoptotic pathway[J]. Exp. Dermatol., 2016, 25 (6) : 440-446. doi: 10.1111/exd.12964 pmid: 26836460
[2]	Meng X, Hu L, Li W. Baicalin ameliorates lipopolysaccharide-induced acute lung injury in mice by suppressing oxidative stress and inflammation via the activation of the Nrf2-mediated HO-1 signaling pathway[J]. Naunyn. Schmiedebergs. Arch. Pharmacol., 2019, 392 (11) : 1421-1433. doi: 10.1007/s00210-019-01680-9
[3]	Chaiprasongsuk A, Panich U. Role of phytochemicals in skin photoprotection via regulation of Nrf2[J]. Front. Pharmacol., 2022, 13: 823881. doi: 10.3389/fphar.2022.823881
[4]	Bollag W B, Aitkens L, White J, et al. Aquaporin-3 in the epidermis: more than skin deep[J]. Am. J. Physiol. Cell Physiol., 2020, 318 (6) : 1144-1153.
[5]	Cao C, Sun Y, Healey S, et al. EGFR-mediated expression of aquaporin-3 is involved in human skin fibroblast migration[J]. Biochem. J., 2006, 400 (2) : 225-234. pmid: 16848764
[6]	Gruber J V, Holtz R. Examining the genomic influence of skin antioxidants in vitro[J]. Mediators Inflamm., 2010, 2010: 230450.
[7]	Li Z, Jiang R, Wang M, et al. Ginsenosides repair UVB-induced skin barrier damage in BALB/c hairless mice and HaCaT keratinocytes[J]. J. Ginseng. Res., 2022, 46 (1) : 115-125. doi: 10.1016/j.jgr.2021.05.001 pmid: 35035244
[8]	Pathak N, Bhaduri A, Bhat K V, et al. Tracking sesamin synthase gene expression through seed maturity in wild and cultivated sesame species—a domestication footprint[J]. Plant. Biol. (Stuttg)., 2015, 17 (5) : 1039-1046. doi: 10.1111/plb.2015.17.issue-5
[9]	Korać R R, Khambholja K M. Potential of herbs in skin protection from ultraviolet radiation[J]. Pharmacogn. Rev., 2011, 5 (10) : 164-173. doi: 10.4103/0973-7847.91114
[10]	Lin T K, Zhong L, Santiago J L. Anti-inflammatory and skin barrier repair effects of topical application of some plant oils[J]. Int. J. Mol. Sci., 2017, 19 (1) : 70. doi: 10.3390/ijms19010070
[11]	Rosalina R, Weerapreeyakul N. An insight into sesamolin: physicochemical properties, pharmacological activities, and future research prospects[J]. Molecules, 2021, 26 (19) : 5849. doi: 10.3390/molecules26195849
[12]	Srisayam M, Weerapreeyakul N, Kanokemedhakul K. Inhibition of two stages of melanin synthesis by sesamol,sesamin and sesamolin[J]. Asian Pacific Journal of Tropical Biomedicine, 2017, 10 (7) : 42-51. doi: 10.4103/2221-1691.273093
[13]	Chen Y, Liu X, Lei X, et al. Premna microphylla Turcz pectin protected UVB-induced skin aging in BALB/c-nu mice via Nrf2 pathway[J]. Int. J. Biol. Macromol., 2022, 215: 12-22. doi: 10.1016/j.ijbiomac.2022.06.076 pmid: 35718142
[14]	Yu J, Sun H, Yang Y, et al. Sesamolin alleviates nonalcoholic fatty liver disease through modulating gut microbiota and metabolites in high-fat and high-fructose diet-fed mice[J]. Int. J. Mol. Sci., 2022, 23 (22) : 13853. doi: 10.3390/ijms232213853
[15]	Zhang L, Zhang S, Song H, et al. Ingestion of collagen hydrolysates alleviates skin chronological aging in an aged mouse model by increasing collagen synthesis[J]. Food Funct., 2020, 11 (6) : 5573-5580. doi: 10.1039/d0fo00153h pmid: 32520042
[16]	Hwang E, Lee D G, Park S H, et al. Coriander leaf extract exerts antioxidant activity and protects against UVB-induced photoaging of skin by regulation of procollagen typeⅠand MMP-1 expression[J]. J. Med. Food, 2014, 17 (9) : 985-995. doi: 10.1089/jmf.2013.2999
[17]	Wu S J, Tung Y J, Yen M H, et al. Chemical composition and anti-aging effects of standardized herbal chicken essence on D-galactose-induced senescent mice[J]. Front. Nutr., 2022, 9: 989067. doi: 10.3389/fnut.2022.989067
[18]	Ho C C, Ng S C, Chuang H L, et al. Extracts of Jasminum sambac flowers fermented by Lactobacillus rhamnosus inhibit H (2) O (2) -and UVB-induced aging in human dermal fibroblasts[J]. Environ. Toxicol., 2021, 36 (4) : 607-619. doi: 10.1002/tox.v36.4
[19]	Lee M J, Agrahari G, Kim H Y, et al. Extracellular superoxide dismutase prevents skin aging by promoting collagen production through the activation of AMPK and Nrf2/HO-1 cascades[J]. J. Invest. Dermatol., 2021, 141 (10) : 2344-2353, 2347. doi: 10.1016/j.jid.2021.02.757
[20]	Ishida Y, Kuninaka Y, Nosaka M, et al. Forensic application of epidermal AQP3 expression to determination of wound vitality in human compressed neck skin[J]. Int. J. Legal. Med., 2018, 132 (5) : 1375-1380. doi: 10.1007/s00414-018-1780-1 pmid: 29356947
[21]	Xie H, Zhou L, Liu F, et al. Autophagy induction regulates aquaporin 3-mediated skin fibroblast ageing[J]. Br. J. Dermatol., 2022, 186 (2) : 318-333. doi: 10.1111/bjd.v186.2
[22]	Li J, Tang H, Hu X, et al. Aquaporin-3 gene and protein expression in sun-protected human skin decreases with skin ageing[J]. Australas. J. Dermatol., 2010, 51 (2) : 106-112. doi: 10.1111/j.1440-0960.2010.00629.x pmid: 20546216

	SOD含量/（U/（mg·prot））	CAT含量/（U/（mg·prot））	GSH-Px含量/（U/（mg·prot））	MDA含量/（nmol/（mg·prot））
Control	4.13±0.25	4.48±0.28	145.36±9.67	1.38±0.10
UVB	2.07±0.20 ^*	1.45±0.10 ^*	87.96±4.99 ^*	3.47±0.36 ^*
L-SES	2.54±0.19 ^*#	2.31±0.15 ^*#	116.65±7.04 ^*#	2.93±0.25 ^*#
M-SES	2.90±0.17 ^*#&	2.88±0.22 ^*#&	128.97±10.97 ^*#&	2.37±0.13^*#&
H-SES	3.60±0.22 ^*#&?	3.62±0.24 ^*#&?	138.99±5.08 ^*#&?	1.89±0.15 ^*#&?
F	187.649	379.155	98.300	168.774
P	<0.001	<0.001	<0.001	<0.001