表面活性剂对PCMX抑菌效果影响研究

doi:10.3969/j.issn.2097-2806.2024.10.008

摘要/Abstract

摘要：

研究了3种表面活性剂（SDS、AES和AEO9）对抑菌剂对氯间二甲苯酚（PCMX）的增溶作用及其复配体系对金黄色葡萄球、大肠杆菌和绿脓杆菌的抑菌效果。结果表明，表面活性剂与PCMX的结合系数受表面活性剂的浓度影响的大小顺序为：AEO9>AES>SDS。通过最小抑菌浓度（MIC）测试考察了表面活性剂对PCMX抑菌效果的影响。当SDS的浓度在临界胶束浓度（cmc）以下时，复配体系中PCMX的MIC维持不变，当SDS浓度进一步增加会引起MIC线性增加。当AES的浓度在cmc以下时，复配体系中PCMX的MIC随AES浓度增加略有降低，在cmc以上AES浓度进一步增加会引起MIC线性增加。当AEO9浓度在1.61 mmol/L及以下时，AEO9浓度增加导致复配体系对金黄色葡萄球菌、大肠杆菌和绿脓杆菌的MIC降低。发现在AEO9的cmc以上存在一段浓度区间，复配体系的抗菌效能随着AEO9浓度增加而提升。

关键词: 对氯间二甲苯酚, 表面活性剂, 临界胶束浓度, 最小抑菌浓度, 结合系数

Abstract:

The solubilization effects of three surfactants, i.e., SDS, AES and AEO9, on PCMX （parachlorometaxylenol） were studied, as well as their combined impact on the antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The results indicated that, the binding coefficient of surfactant with PCMX was influenced by the concentration of surfactant in the following order: AEO9>AES>SDS. The influences of surfactants on the antibacterial effect of PCMX were examined through minimum inhibitory concentration （MIC） testing. For the SDS concentrations below its critical micelle concentration （cmc）, the MIC of the PCMX complex remained unchanged. However, a further increase in SDS concentration beyond the cmc would lead to a linear increase in MIC. In the case of AES below cmc, the MIC of the PCMX complex was slightly decreased with the concentration of AES, while the AES in the concentration range above cmc would cause a linear increase of MIC. When the concentration of AEO9 was≤1.61 mmol/L, the increase of AEO9 concentration would lead to a decrease in MIC against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Above the cmc of AEO9, there was a concentration range in which the increase of AEO9 concentration could enhance the antibacterial efficacy of the system. This study might provide valuable insights into the synergism between surfactants and PCMX in antibacterial effectiveness.

Key words: PCMX, surfactant, critical micelle concentration, minimum inhibitory concentration, binding coefficient

中图分类号:

TQ423

刘庆刚. 表面活性剂对PCMX抑菌效果影响研究[J]. 日用化学工业（中英文）, 2024, 54(10): 1211-1217.

Qinggang Liu. Study on the influences of surfactants on the antibacterial effect of PCMX[J]. China Surfactant Detergent & Cosmetics, 2024, 54(10): 1211-1217.

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表面活性剂类型	cmc/（mmol/L）
表面活性剂类型	纯水	PCMX溶液（c=1.28 mmol/L）
SDS	7.98	5.89
AES	2.58	2.06
AEO9	0.177	0.017 7

c（SDS）/（mmol/L）	c（PCMX）/（mmol/L）	U
0	2.33	1.00
2.77	2.79	1.20
8.67	5.40	2.32
17.34	13.00	5.57
26.01	19.90	8.58
34.68	26.90	11.55
52.01	38.80	16.70
69.35	51.20	22.02
138.71	98.30	42.27
208.06	149.00	63.88
277.41	205.00	88.24
346.76	278.00	119.32

c（AES）/（mmol/L）	c（PCMX）/（mmol/L）	U
0	2.33	1.00
1.31	4.30	1.85
6.54	8.99	3.86
13.09	15.20	6.56
19.63	20.60	8.87
26.18	26.70	11.46
39.27	37.90	16.28
52.36	50.40	21.69
104.71	92.90	39.96
157.07	133.00	57.28
209.42	175.00	75.24
261.78	215.00	92.50

c（AEO9）/（mmol/L）	c（PCMX）/（mmol/L）	U
0	2.33	1.00
0.32	2.28	0.98
1.61	2.61	1.12
3.22	3.81	1.64
8.05	9.02	3.88
16.10	14.60	6.28
24.15	21.50	9.24
32.21	27.90	12.00
64.41	58.10	24.98
96.62	89.50	38.46
128.82	132.00	56.59
161.03	177.00	76.24

表面活性剂	MIC/（mmol/L）
表面活性剂	金黄色葡萄球菌	大肠杆菌	绿脓杆菌
SDS	0.520~0.347	>178	>178
AES	1.030~0.515	>132	>132
AEO9	>90.5	> 90.5	> 90.5