4种原料在高渗脱水细胞模型中的作用研究

doi:10.3969/j.issn.2097-2806.2024.10.009

摘要/Abstract

摘要：

为探究透明质酸钠、泛醇、马齿苋提取物和金盏花提取物对高渗脱水诱导人永生化角质形成细胞（HaCaT）损伤的保护作用。通过检测细胞活率筛选4种原料安全作用浓度，采用ELISA法测定了透明质酸（HA）的分泌量。在mRNA水平上检测HaCaT屏障功能相关基因（OVOL1，EREG，TGM1，TGM2，IVL，IRF6，THBS1，CASP14）的表达情况，探究4种原料对屏障功能相关基因的调节作用。结果发现，4种原料能提高高渗脱水后的细胞活率，促进HA的分泌，改善高渗脱水后屏障功能相关基因的表达情况，其中泛醇和金盏花提取物效果较好。结果表明，4种原料对高渗脱水细胞模型均有一定的保护作用，为其在化妆品中的应用提供数据支撑。

关键词: 高渗脱水细胞模型, 保护作用, 屏障修复, 化妆品功效评价, 舒缓功效原料

Abstract:

It aims to investigate the protective effects of sodium hyaluronate, panthenol, Portulaca oleracea L. and Calendula officinalis L. on hyperosmotic dehydration-induced injury of human immortalized keratinocytes （HaCaT）. The safety mass concentrations of four raw materials were screened by detecting cell viability, and the secretion of hyaluronic acid （HA） was determined using the ELISA method. The expression of HaCaT barrier function related genes （OVOL1, EREG, TGM1, TGM2, IVL, IRF6, THBS1, CASP14） was detected at the mRNA level to explore the regulatory effect of four raw materials on these genes. The results demonstrate that pretreatment with the four kinds of raw materials could increase the cell viability after hyperosmotic dehydration, promote the secretion of HA, and improve the expression of barrier function related genes after hyperosmotic dehydration, among which panthenol and Calendula officinalis L. are better. The results show that the four raw materials have a certain protective effect on the hyperosmotic dehydration cell model, which provides data support for its application in cosmetics.

Key words: hypertonic dehydration cell model, protective effect, barrier repair, efficacy evaluation of cosmetics, soothing ingredients

中图分类号:

TQ658

高媛媛, 谭淇丹, 曹晨灿, 陈海峰, 加裕盈, 刘蕾. 4种原料在高渗脱水细胞模型中的作用研究[J]. 日用化学工业（中英文）, 2024, 54(10): 1218-1226.

Yuanyuan Gao, Qidan Tan, Chencan Cao, Haifeng Chen, Yuying Jia, Lei Liu. Study on the effect of four kinds of raw materials in hypertonic dehydration cell model[J]. China Surfactant Detergent & Cosmetics, 2024, 54(10): 1218-1226.

图/表 8

参考文献 16

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No.	Gene name	Primer sequence
1	OVOL1	Forward: 5’-GCCACATGAAGTGTCACAACGA-3’
1	OVOL1	Reverse: 5’-ACTCCTCACACACGTACAGCTTG-3’
2	EREG	Forward: 5’-TTTCCATCTTCTACAGGCAGTCC-3’
2	EREG	Reverse: 5’-CATCGGACACCAGTATAACCCAC-3’
3	IVL	Forward: 5’-GTTCCTCCTCCAGTCAATACCCA-3’
3	IVL	Reverse: 5’-CACATTCTTGCTCAGGCAGTCC-3’
4	THBS1	Forward: 5’-GAGAATGCTGTCCTCGCTGTT-3’
4	THBS1	Reverse: 5’-CTCGTAGAACAGGAGGTCCACTC-3’
5	TGM1	Forward: 5’-ATGTCTCAGGCCACGTCAAG-3’
5	TGM1	Reverse: 5’-CCGGAAGACGACATTGGTGA-3’
6	TGM2	Forward: 5’-CTCACCCAGCAGGGCTTTAT-3’
6	TGM2	Reverse: 5’-CCATCTTCAAACTGCCCAAAA-3’
7	IRF6	Forward: 5’-AGATGGAAGTACCCCAGGCAC-3’
7	IRF6	Reverse: 5’-AGGCTGACGGGACCAAAGA-3’
8	CASP14	Forward: 5’-GTCAGTTGTGCCTTCGTGGTA-3’
8	CASP14	Reverse: 5’-GGTTTGTGGGCTGTCTTTGATG-3’

Stage 1	Stage 2 （40 cycles）	Stage 3 （Melting curve）
95 ℃, 30 sec, predenaturation	95 ℃, 15 sec, denaturation	65 ℃→95 ℃
	60 ℃, 30 sec, annealing/extension	Collect fluorescent signal every 0.5 ℃ of temperature increase