鞣花酸纳米银颗粒的绿色合成、表征及抗菌活性研究

doi:10.3969/j.issn.2097-2806.2026.09.006

摘要/Abstract

摘要：

以鞣花酸为还原剂和稳定剂，采用一锅法绿色合成鞣花酸修饰的纳米银颗粒（EA-AgNPs），同时对EA-AgNPs进行紫外-可见分光光度、粒径、Zeta电位、透射电子显微镜、X射线衍射及傅里叶变换红外光谱的系统表征，并评价了EA-AgNPs的体外抗菌活性。结果表明，EA-AgNPs的制备条件为：鞣花酸与硝酸银的摩尔比1∶1，在85 ℃、pH=10的碱性条件下，加热搅拌1 h，转速为200 r/min。该纳米银颗粒呈球形，平均粒径为20~50 nm。Zeta电位为-25 mV。透射电子显微镜和X射线衍射分析显示该颗粒具有良好的分散性和晶体结构。抗菌实验证实，EA-AgNPs对大肠杆菌（E. coli）、金黄色葡萄球菌（S. aureus）均具有显著抗菌活性，其最小抑菌浓度（MIC）分别为1.56和6.25 μg/mL，明显优于游离鞣花酸（大肠杆菌提高了40倍，金黄色葡萄球菌提高了5倍）。时间-杀菌曲线进一步证实其快速杀菌能力，尤其对大肠杆菌效果显著。细胞毒性测试表明，EA-AgNPs在抗菌有效浓度下对哺乳动物3T3细胞毒性较低，生物相容性良好。综上，EA-AgNPs具有较好的体外抗菌活性，并具有较好的生物相容性。

关键词: 鞣花酸纳米银颗粒, 绿色合成, 抗菌

Abstract:

Ellagic acid-modified silver nanoparticles （EA-AgNPs） were synthesized through one-pot green synthesis approach by using ellagic acid as both reducing agent and stabilizer. The product of EA-AgNPs was characterized by ultraviolet-visible spectrophotometry, particle-size distribution analysis, zeta potential measurement, transmission electron microscopy （TEM）, X-ray diffraction （XRD）, and Fourier transform infrared spectroscopy （FTIR）. Its antibacterial activity was also investigated. The optimized conditions for preparation of EA-AgNPs were obtained, in which the molar ratio of ellagic acid: silver nitrate was 1∶1, the pH value was 10, and the mixture was heated at 85 ℃ while stirred at stirring speed of 200 r/min for 1 hour. The nanoparticles were spherical, with average particle size of 20-50 nm. The zeta potential was measured as -25 mV. TEM and XRD analyses revealed that the nanoparticles exhibited good dispersity and crystalline structure. The antibacterial tests demonstrated that EA-AgNPs exhibited significant antibacterial activities against Escherichia coli （E. coli） and Staphylococcus aureus （S. aureus）, with minimum inhibitory concentrations （MIC） of 1.56 and 6.25 μg/mL, respectively. Such antibacterial activities were remarkably superior to those of free ellagic acid, showing 40-fold improvement against E. coli and 5-fold improvement against S. aureus. Time-kill curve assays further confirmed its rapid bactericidal efficacy, particularly against E. coli. Cytotoxicity tests showed that EA-AgNPs exhibited low toxicity to mammalian 3T3 cells at effective antibacterial concentrations, indicative of good biocompatibility. In conclusion, EA-AgNPs exhibited notable in vitro antibacterial activity and favorable biocompatibility. This work could provide theoretical support and technical reference for high-value utilization of polyphenols derived from agricultural by-products.

Key words: ellagic acid-modified silver nanoparticle, green synthesis, antibacterial

中图分类号:

TQ658

冯淑瑶, 李佳宁, 王紫鹃, 姜菲, 高振珅, 张海娟. 鞣花酸纳米银颗粒的绿色合成、表征及抗菌活性研究[J]. 日用化学工业（中英文）, 2026, 56(9): 1180-1187.

Shuyao Feng, Jianing Li, Zijuan Wang, Fei Jiang, Zhenshen Gao, Haijuan Zhang. Green synthesis, characterization and antibacterial activity of ellagic acid-modified silver nanoparticles[J]. China Surfactant Detergent & Cosmetics, 2026, 56(9): 1180-1187.

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