荔枝草提取物抗氧化及抗炎活性研究

doi:10.3969/j.issn.2097-2806.2025.10.009

摘要/Abstract

摘要：

为了获得荔枝草中潜在的抗氧化和抗炎活性的功效成分，以总黄酮和总酚酸含量为导向，筛选最佳大孔树脂洗脱工艺，制备得到荔枝草提取物。使用DPPH自由基清除实验和ABTS总抗氧能力实验评估荔枝草提取物的抗氧化功效；使用UVB诱导的HaCaT细胞模型和2, 4-二硝基氟苯（DNFB）诱导的皮炎小鼠模型，探究荔枝草提取物的抗炎功效。结果表明，荔枝草提取物中总黄酮含量为36.42%±0.45%，总酚酸含量为21.32%±0.82%；DPPH和ABTS半数抑制的质量浓度分别为（49.09±0.10） μg/mL和（84.54±0.99） μg/mL；在HaCaT细胞模型上，荔枝草提取物可显著抑制细胞炎症因子IL-6，IL-1α和TNF-α的分泌量；在皮炎小鼠模型上，荔枝草提取物可有效缓解耳部皮肤红肿、溃烂，显著减少炎症细胞数量，减少肥大细胞脱颗粒，降低血清中IFN-γ，IL-4，IL-5，IgE，IL-17等因子的表达量，降低胸腺系数和脾脏系数。

关键词: 荔枝草提取物, 抗氧化, 小鼠, 抗炎

Abstract:

In order to obtain the active components of Salvia plebeia R. Br. for antioxidant and anti-inflammatory activity, the macroporous resin elution process was selected based on the content of total flavonoids and phenolic acid. The antioxidant activity of Salvia plebeia R. Br. extracts （SE） was detected by DPPH free radical scavenging method and ABTS total antioxidant capacity method. UVB-induced human skin immortalized keratinocytes and mouse dermatitis model constructed with 2, 4-dinitrofluorobenzene （DNFB） solution were used to test the anti-inflammatory effect of SE. The total flavonoids content of SE is 36.42%±0.45% and the total phenolic acid content is 21.32%±0.82%. The half maximal inhibitory mass concentrations of DPPH and ABTS are （49.09±0.10） μg/mL and （84.54±0.99） μg/mL, respectively. In vitro, SE can inhibit the secretion of IL-6, IL-1α and TNF-α in human immortalized keratinocytes. In vivo, SE can effectively relieve ear skin redness and swelling, and reduce the number of inflammatory cells and mast cells degranulation, as well as has significant inhibitory effects on the secretion of IFN-γ, IL-4, IL-5, IgE, and IL-17 in mice. In addition, SE can reduce the thymus index and spleen index in mice. This study proves that SE has excellent antioxidant and anti-inflammatory effects, which can provide a basis for high value utilization of SE in the field of daily chemicals.

Key words: Salvia plebeia R. Br. extracts, antioxidant, mice, anti-inflammation

中图分类号:

TQ658

曹贤贤,袁永雷,王飞飞. 荔枝草提取物抗氧化及抗炎活性研究[J]. 日用化学工业（中英文）, 2025, 55(10): 1291-1298.

Xianxian Cao,Yonglei Yuan,Feifei Wang. Study on the antioxidant and anti-inflammatory activities of extracts from Salvia plebeia R. Br.[J]. China Surfactant Detergent & Cosmetics, 2025, 55(10): 1291-1298.

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参考文献 18

[1]	王继锋, 颜娓娓, 徐佳馨, 等. 荔枝草的化学成分及药理作用研究新进展[J]. 湖南中医药大学学报, 2018, 38 (4) : 482-485.
[2]	亢文佳, 富艳彬, 李达翃, 等. 荔枝草的化学成分研究[J]. 中草药, 2015, 46 (11) : 1589-1592.
[3]	于佳慧, 舒淇, 王淇漩, 等. 荔枝草化学成分、药理作用与应用研究进展[J]. 特产研究, 2024, 34: 997-1014.
[4]	Zou Y H, Zhao L, Xu Y K, et al. Anti-inflammatory sesquiterpenoids from the traditional Chinese medicine Salvia plebeia: regulates pro-inflammatory mediators through inhibition of NF-κB and Erk1/2 signaling pathways in LPS-induced Raw264.7 cells[J]. Journal of Ethnopharmacology, 2018, 210: 95-106. doi: 10.1016/j.jep.2017.08.034
[5]	Hyun W K, Taewoong R, Young J S, et al. Isolation of phytochemicals from Salvia plebeia using countercurrent chromatography coupled with reversed-phase HPLC[J]. Natural Product Sciences, 2020, 26 (3) : 236-243. doi: 10.20307/nps.2020.26.3.236
[6]	Huang J L, Fan R Z, Zou Y H, et al. Salviplenoid A from Salvia plebeia attenuates acute lung inflammation via modulating NF-κB and Nrf2 signaling pathways[J]. Phytotherapy Research, 2021, 35 (3) : 1559-1571. doi: 10.1002/ptr.v35.3
[7]	Xiong G H, Deng Y Y, Cao Z G, et al. The hepatoprotective effects of Salvia plebeia R. Br. extract in zebrafish (Danio rerio)[J]. Fish & Shellfish Immunology, 2019, 95: 399-410.
[8]	Mina K, Ji Y K, Hee S Y, et al. Nepetoidin B from Salvia plebeia R. Br. inhibits inflammation by modulating the NF-κB and Nrf2/HO-1 signaling pathways in macrophage cells[J]. Antioxidants, 2021, 10: 1208. doi: 10.3390/antiox10081208
[9]	Liang Y Y, Wan X H, Niu F J, et al. Salvia plebeia R. Br.: an overview about its traditional uses, chemical constituents, pharmacology and modern applications[J]. Biomedicine & Pharmacotherapy, 2020, 121: 109589. doi: 10.1016/j.biopha.2019.109589
[10]	Ning M, Chen K, Wang Y H, et al. Dihydrohomoplantagin and homoplantaginin, major flavonoid glycosides from Salvia plebeia R. Br. inhibit OxLDL-induced endothelial cell injury and restrict atherosclerosis via activating Nrf2 anti-oxidation signal pathway[J]. Molecules, 2022, 27: 1990. doi: 10.3390/molecules27061990
[11]	Afraa A Z, Ramya P, Krishna M, et al. Computational screening of natural compounds from Salvia plebeia R. Br. for inhibition of SARS-CoV-2 main protease[J]. Vegetos, 2022, 35 (2) : 345-359. doi: 10.1007/s42535-021-00304-z
[12]	Wu F H, Wang H, Li J, et al. Homoplantaginin modulates insulin sensitivity in endothelial cells by inhibiting inflammation[J]. Biological & Pharmaceutical Bulletin, 2012, 35 (7) : 1171-1177.
[13]	徐蕊, 袁永雷, 曲丽萍. 地肤子总黄酮脱色工艺及抗氧化活性研究[J]. 食品工业, 2023, 44 (5) : 6-10.
[14]	陈江龙, 吴丽丽, 周丽红, 等. 橘皮总多酚的提取及其抗氧化活性研究[J]. 食品工业, 2023, 44 (8) : 1-4.
[15]	Irina G M, Constantin A. Analytical methods used in determining antioxidant activity: A review[J]. International Journal of Molecular Sciences, 2021, 22: 3380. doi: 10.3390/ijms22073380
[16]	Seon G B, Hyung J L, Yeong S W, et al. Effect of Ampelopsis brevipedunculata (Maxim.) Trautv extract on a model of atopic dermatitis in HaCaT cells and mice[J]. Food Science & Nutrition, 2023, 11: 6616-6625. doi: 10.1002/fsn3.v11.10
[17]	Li W Z, Liu X X, Shi Y J, et al. Unveiling the mechanism of high sugar diet induced advanced glycosylation end products damage skin structure via extracellular matrix-receptor interaction pathway[J]. Journal of Cosmetic Dermatology, 2024, 23 (7) : 2496-2508. doi: 10.1111/jocd.v23.7
[18]	Zhang M, Hui H C, Zhao X R, et al. Protective effects of dioscin against Parkinson’s disease via regulating bile acid metabolism through remodeling gut microbiome/GLP-1signaling[J]. Journal of Pharmaceutical Analysis, 2023, 13: 1153-1167. doi: 10.1016/j.jpha.2023.06.007 pmid: 38024855