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日用化学工业（中英文）

日用化学工业（中英文） ›› 2024, Vol. 54 ›› Issue (7): 777-783.doi: 10.3969/j.issn.2097-2806.2024.07.004

• 开发与应用 • 上一篇    下一篇

氧化苦参碱对小鼠皮肤屏障功能障碍的修复作用研究

刘兆亿1,陈鑫宇2,王艳2,3,4,*(),李雪5,郭若曦6,张晗6   

  1. 1.天津中医药大学 体育健康学院，天津 301617
    2.天津中医药大学 中药制药工程学院，天津 301617
    3.天津市中药智能制药与绿色制药重点实验室，天津 301617
    4.现代中医药海河实验室，天津 301617
    5.天津医科大学总医院，天津 300052
    6.天津尚美化妆品有限公司，天津 300385
  • 收稿日期:2023-12-12 修回日期:2024-06-07 出版日期:2024-07-22 发布日期:2024-07-26
  • 基金资助:
    国家自然科学基金青年科学基金项目(81602773);天津市技术创新引导专项（基金）(23YDTPJC00590);中国工程院项目(2023-XBZD-11-01);现代中医药海河实验室2023年第一批“揭榜挂帅”科技项目

Study on the reparative effects of oxymatrine on impaired skin barrier function in mice

Zhaoyi Liu1,Xinyu Chen2,Yan Wang2,3,4,*(),Xue Li5,Ruoxi Guo6,Han Zhang6   

  1. 1. School of Sport and Health, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
    2. School of Chinese Medicine and Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
    3. Tianjin Key Laboratory of Intelligent and Green Pharmaceuticals for Traditional Chinese Medicine, Tianjin 301617, China
    4. Haihe Laboratory of Modern Chinese Medicine, Tianjin 301617, China
    5. Department of Nuclear Medicine Tianjin Medical University General Hospital, Tianjin 300052, China
    6. Tianjin Shangmei Cosmetics Co., Ltd, Tianjin 300381, China
  • Received:2023-12-12 Revised:2024-06-07 Online:2024-07-22 Published:2024-07-26
  • Contact: *E-mail: paozhijiaoxue@126.com.

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摘要：

探讨氧化苦参碱（OMT）乳膏对小鼠皮肤屏障功能的修复作用。将70只雄性ICR小鼠随机分为7组，除正常组小鼠外，采用胶带粘贴法模拟机械损伤，复制皮肤屏障功能障碍小鼠模型。造模后经皮连续给药5天，拍摄照片并测定造模区域的经表皮失水量（TEWL）。实验结束后，HE染色观察皮肤组织形态学变化，酶联免疫吸附法（ELISA）检测皮肤组织中肿瘤坏死因子α （TNF-α）、白细胞介素-8 （IL-8）的含量，通过实时定量PCR （RT-qPCR）检测紧密连接蛋白（ZO-1）、丝聚蛋白（FLG）、水通道蛋白3 （AQP3） mRNA的表达量，通过Western blot检测屏障相关蛋白如封闭蛋白（OCC）、FLG、紧密连接蛋白（Claudin）的表达量。结果显示，与模型组相比，OMT各剂量组均能降低皮肤TEWL，减轻炎症浸润，改善皮肤病理状态，同时高剂组皮肤组织中TNF-α等炎症因子的含量显著降低（P<0.05），ZO-1、FLG mRNA及OCC、FLG、Claudin蛋白的表达显著升高（P<0.05）。因此，OMT具有修复皮肤屏障功能的作用。

关键词: 氧化苦参碱, 皮肤屏障, 小鼠

Abstract:

This study investigates the reparative effects of oxymatrine （OMT） cream on the skin barrier function in mice. Seventy male ICR mice were randomly divided into seven groups. Except for the normal group, mechanical damage was induced using tape-stripping to create a mouse model of impaired skin barrier function. Following model induction, continuous topical administration of OMT was administered for 5 days, during which time photographs were taken, and transepidermal water loss （TEWL） was measured in the model area. After completing the experiment, morphological changes in skin tissue were observed using Hematoxylin and Eosin （HE） staining. Enzyme-linked immunosorbent assay （ELISA） was used to determine the levels of tumor necrosis factor-alpha （TNF-α） and interleukin （IL）-8 in skin tissue. Real-time quantitative PCR （RT-qPCR） was used to assess the expression levels of tight junction protein （ZO-1）, filaggrin （FLG）, and aquaporin 3 （AQP3） mRNA. Additionally, Western blot analysis was performed to measure the expression levels of occludin （OCC）, FLG, and claudin-1. The results indicated that all OMT dosage groups exhibited a reduction in skin TEWL, alleviation of inflammatory infiltration, and improvement in skin pathological conditions compared to the model group. Moreover, the high-dosage group showed a significant decrease in the levels of inflammatory factors such as TNF-α （P<0.05）, and a significant increase in the expression of ZO-1, FLG mRNA, OCC, FLG, and Claudin （P<0.05）. OMT was found to possess reparative effects on skin barrier function, with its mechanism may be associated with the inhibition of inflammatory factors and the upregulation of barrier-related protein expression.

Key words: oxymatrine, skin barrier, mice

中图分类号: 

  • TQ658