薰衣草精油和亚洲薄荷精油促毛发生长作用的研究

doi:10.3969/j.issn.1001-1803.2020.08.005

摘要/Abstract

摘要：

通过体外细胞实验和脱发小鼠模型,研究了薰衣草精油与亚洲薄荷精油对毛发生长的促进作用。通过细胞实验考察两种精油对人永生角质形成细胞血管内皮生长因子（VEGF）和角质形成细胞生长因子（KGF）分泌的影响;通过小鼠模型考察两种精油对新生毛发生长长度、脱毛区皮肤组织学变化、毛囊数目、真皮厚度、皮肤VEGF和KGF表达的影响。结果表明,两种精油均能促进毛发伸长,增加脱毛区皮肤毛囊数目和真皮层厚度,延长毛发处于生长期的时间,但二者的作用机制不同;薰衣草精油促进KGF的分泌,亚洲薄荷精油则促进VEGF的分泌,动物实验结果与细胞实验结果相符。说明薰衣草精油和亚洲薄荷精油均有促毛发生长的功效。

关键词: 薰衣草精油, 亚洲薄荷精油, 毛发生长, 人永生角质形成细胞, 脱发小鼠模型

Abstract:

The effects of lavender （Lavandula angustifolia） essential oil and Asian mint （Mentha asiatica） essential oil on hair growth were studied by cell experiment in vitro and hair loss mouse model. The effects of two essential oils on the secretion of vascular endothelial growth factor （VEGF） and keratinocyte growth factor （KGF） of human immortalized keratinocytes were detected by cell experiment. The effects of two essential oils on hair regrowth length, histological changes of skin in depilated area, number of hair follicles, thickness of dermis, and expressions of skin VEGF and KGF were studied by mouse model. The results show that both lavender essential oil and Asian mint essential oil can promote hair elongation, increase the number of hair follicles and dermal thickness in the depilated area, and prolong the anagen hair cycle in mice. However, their mechanisms might be different. Lavender essential oil promotes the secretion of KGF, while Asian mint essential oil increases the secretion of VEGF. The results of animal experiments are consistent with those of cell experiments. It demonstrates that both lavender essential oil and Asian mint essential oil have hair-promoting effects.

Key words: lavender essential oil, Asian mint essential oil, hair growth, human immortalized keratinocytes, hair loss mouse model

中图分类号:

TQ658

李蕊,张楠,姚雷,李玉红. 薰衣草精油和亚洲薄荷精油促毛发生长作用的研究[J]. 日用化学工业, 2020, 50(8): 536-541.

LI Rui,ZHANG Nan,YAO Lei,LI Yu-hong. Study on the hair growth promotion effect of lavender essential oil and Asian mint essential oil[J]. China Surfactant Detergent & Cosmetics, 2020, 50(8): 536-541.

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ρ （精油） / （mg·L^-1）	薰衣草精油		亚洲薄荷精油
ρ （精油） / （mg·L^-1）	RGR/%	毒性分级	RGR/%	毒性分级
0	100.00± 7.40	0	100.00±12.49	0
0.01	88.98± 8.10	1	113.41±19.52	0
0.1	84.71±10.66	1	115.60± 4.85	0
1	86.23± 8.41	1	96.52± 8.69	1
10	95.18±12.77	1	106.96± 2.08	0
50	80.58± 1.19	1	98.97±13.75	1
100	91.87± 3.26	1	100.26±11.72	0
500	76.31± 5.64^*	2	89.04± 7.59	1

	ρ （VEGF） / （μg·L^-1）
	0 mg/L	0.01 mg/L	0.1 mg/L	1 mg/L	10 mg/L	100 mg/L
薰衣草精油	3.70±0.11	3.97±0.08	4.21±0.47	4.02±0.15	3.95±0.15	4.14±0.47
亚洲薄荷精油	2.66±1.14	4.74±1.77^*	4.54±1.47	3.35±0.54	4.97±1.02^*	3.67±1.04

	ρ （KGF） / （μg·L^-1）
	0 mg/L	0.01 mg/L	0.1 mg/L	1 mg/L	10 mg/L	100 mg/L
薰衣草精油	2.84±0.20	3.60±0.56^**	3.39±0.22^*	3.41±0.26^*	3.62±0.21^**	3.73±0.25^**
亚洲薄荷精油	2.57±0.51	2.59±0.60	2.52±0.62	2.76±0.13	3.12±0.77	2.57±0.58

组别	毛发长度/mm
组别	12 d	15 d	18 d	21 d
对照组	1.79±0.19	2.88±0.35	4.91±0.49	5.75±0.51
阳性对照组	2.09±0.30^**	3.37±0.58^**	5.11±0.49	5.83±0.56
1%薰衣草精油组	1.96±0.25^*	3.50±0.93^**	5.22±0.69^*	6.08±0.52^*
5%薰衣草精油组	1.94±0.31^*	2.78±0.56	4.48±0.80^**	5.34±0.76^**
10%薰衣草精油组	1.89±0.27	2.94±0.38	4.84±0.72	5.56±0.76
1%亚洲薄荷精油组	2.09±0.27^**	3.06±0.37	4.77±0.60	5.63±0.48
5%亚洲薄荷精油组	1.94±0.51^*	3.01±0.28	4.66±0.47	5.70±0.37
10%亚洲薄荷精油组	1.77±0.17	3.15±0.61^*	4.55±0.62^*	5.30±0.78^**

组别	毛囊数量/（个/视野）	真皮厚度/μm
对照组	10.60± 2.51	246.19± 20.02
阳性对照组	28.20±11.30^*	336.91± 39.68^*
1%薰衣草精油组	35.80±16.81^**	352.38± 41.41^*
5%薰衣草精油组	24.00± 6.53	331.99± 39.85^*
10%薰衣草精油组	29.67±11.43^*	300.70± 50.90
1%亚洲薄荷精油组	31.00±11.92^*	366.67± 53.98^**
5%亚洲薄荷精油组	30.50±20.14^*	390.48±110.77^**
10%亚洲薄荷精油组	15.80± 5.22	302.38± 48.42