关键词: 黄芩, 丹参, 面膜剂, 痤疮, 抗菌活性

Abstract:

Acne is a common skin disease, and the occurrence of acne is closely related to Propionibacterium acnes. Research shows that Scutellaria baicalensis Georgi and Salvia miltiorrhiza Bge. have good antibacterial activities, especially to Propionibacterium acnes. The facial mask combined S. baicalensis Georgi with S. miltiorrhiza Bge. was prepared, and its forming process was optimized through response surface test. A rapid analysis technology for active ingredients was established using high performance liquid chromatography （HPLC）, and the experiments were held to investigate the antibacterial activities of Propionibacterium acnes and Staphylococcus aureus in vitro. The results show that three main factors affecting the forming process are glycerol, 1, 3-butanediol and hydroxyethyl cellulose identified by single factor test. Box-Behnken test and response surface method are used to optimize the variation parameters of the three significant factors, and its optimal process conditions. The twelve indicative activities in the facial mask are identified by HPLC, and the rapid quality analysis method and its chromatographic conditions are established. Also, the bacteriostatic test in vitro of the facial mask confirms that it shows certain bacteriostatic effects on Propionibacterium acens and Staphylococcus aureus, and can exert effectively and continuously within ten hours. When it is combined with positive drug DP-300, it shows better synergistic or additive bacteriostatic effects, which are considered as the mild bacteriostatic effects due to facial mask.

Key words: Scutellaria baicalensis Georgi, Salvia miltiorrhiza Bge., facial mask, acne, antibacterial activity