水解透明质酸锌的抗痤疮功效研究

doi:10.3969/j.issn.1001-1803.2023.02.006

摘要/Abstract

摘要：

探究了水解透明质酸锌（HA-ZN）在控油与保湿方面的体外生物活性。通过MTT法检测了不同质量浓度的HA-ZN对SZ95和HaCaT的细胞活性的影响，利用油红染色法检测HA-ZN对SZ95细胞内的油脂含量的影响，并且利用体外抑菌实验检测了HA-ZN的抑菌作用。同时，利用免疫荧光法检测HaCaT细胞中HAS2和AQP3表达的变化。结果表明，HA-ZN可以提升锌的安全用量。0.2 mg/mL和0.225 mg/mL的HA-ZN可以抑制SZ95细胞的油脂生成。5 mg/mL的HA-ZN可以抑制痤疮丙酸杆菌和糠秕马拉色菌的生长。同时，0.225 mg/mL的HA-ZN可促进HaCaT细胞表达HAS2和AQP3。HA-ZN具有较好的控油，抑制痤疮相关的菌群的作用，同时具有一定的保湿效果，提示其具有一定的抗痤疮的功效。

关键词: 痤疮, 皮肤保湿, 锌盐, 水解透明质酸锌

Abstract:

In this study, the effects of hydrolysed zinc hyaluronan （HA-ZN） on lipid production and skin hydration were revealed. The HaCaT and SZ95 cell lines were applied to evaluate the toxicity and beneficial effect of hydrolysed zinc hyaluronan. Pro-acne agents, arachidonic acid was used to induce the lipid production of SZ95 and the effect of hydrolysed zinc hyaluronan was detected. At the same time, in vitro antibacterial experiment against acne-related bacteria and the expression of skin hydration involved proteins were estimated. At the identical concentration of zinc ion, hydrolysed zinc hyaluronan exhibits less toxicity than zinc chloride, suggesting a higher safe dosage of zinc ion. In addition, hydrolysed zinc hyaluronan possesses the ability of alleviating lipid production in SZ95, as well as inhibiting the growth of Propionibacterium acnes and Malassezia furfur in vitro. The expression of AQP3 and HAS2 is also augmented in hydrolysed zinc hyaluronan treated group. The results indicate that hydrolysed zinc hyaluronan might be a promising drug candidate against acne vulgaris, averting the side effects of existing drugs such as dry skin.

Key words: acne vulgaris, skin hydration, zinc salt, hydrolysed zinc hyaluronan

中图分类号:

TQ658

陈帆,杨君,杨素萍,吴越. 水解透明质酸锌的抗痤疮功效研究[J]. 日用化学工业（中英文）, 2023, 53(2): 165-170.

Chen Fan,Yang Jun,Yang Suping,Wu Yue. Hydrolysed zinc hyaluronan, a novel anti-acne candidate[J]. China Surfactant Detergent & Cosmetics, 2023, 53(2): 165-170.

图/表 4

参考文献 25

[1]	Sitohang I B S, Yahya Y F, Simanungkalit R, et al. Efficacy and tolerability of topical nicotinamide plus antibacterial adhesive agents and zinc-pyrrolidone carboxylic acid versus placebo as an adjuvant treatment for moderate acne vulgaris in indonesia: a multicenter, double-blind, randomized, controlled trial[J]. The Journal of Clinical and Aesthetic Dermatology, 2020, 13 (7):27-31.
[2]	Knutsen-Larson S, Dawson A L, Dunnick C A, et al. Acne vulgaris: pathogenesis, treatment, and needs assessment[J]. Dermatologic Clinics, 2012, 30 (1):99-106. doi: 10.1016/j.det.2011.09.001 pmid: 22117871
[3]	Saitta P, Keehan P, Yousif J, et al. An update on the presence of psychiatric comorbidities in acne patients, Part 1: overview of prevalence[J]. Cutis, 2011, 88 (1):33-40. pmid: 21877504
[4]	Kurokawa I, Danby F W, Ju Q, et al. New developments in our understanding of acne pathogenesis and treatment[J]. Experimental Dermatology, 2009, 18 (10):821-832. doi: 10.1111/j.1600-0625.2009.00890.x pmid: 19555434
[5]	Szabó K, Kemény L. Studying the genetic predisposing factors in the pathogenesis of acne vulgaris[J]. Human Immunology, 2011, 72 (9):766-773. doi: 10.1016/j.humimm.2011.05.012 pmid: 21669244
[6]	Zouboulis C C, Eady A, Philpott M, et al. What is the pathogenesis of acne?[J]. Experimental Dermatology, 2005, 14 (2):143-152. doi: 10.1111/j.0906-6705.2005.0285a.x pmid: 15679586
[7]	Oláh A, Markovics A, Szabó-Papp J, et al. Differential effectiveness of selected non-psychotropic phytocannabinoids on human sebocyte functions implicates their introduction in dry/seborrhoeic skin and acne treatment[J]. Experimental Dermatology, 2016, 25 (9):701-707. doi: 10.1111/exd.13042 pmid: 27094344
[8]	Zouboulis C C. Acne and sebaceous gland function[J]. Clinics in Dermatology, 2004, 22 (5):360-366. doi: 10.1016/j.clindermatol.2004.03.004
[9]	Shi V Y, Leo M, Hassoun L, et al. Role of sebaceous glands in inflammatory dermatoses[J]. Journal of the American Academy of Dermatology, 2015, 73 (5):856-863. doi: 10.1016/j.jaad.2015.08.015 pmid: 26386632
[10]	Xu H, Li H. Acne, the skin microbiome, and antibiotic treatment[J]. American Journal of Clinical Dermatology, 2019, 20 (3):335-344. doi: 10.1007/s40257-018-00417-3 pmid: 30632097
[11]	Blasiak R C, Stamey C R, Burkhart C N, et al. High-dose isotretinoin treatment and the rate of retrial, relapse, and adverse effects in patients with acne vulgaris[J]. JAMA Dermatology, 2013, 149 (12):1392-1398. doi: 10.1001/jamadermatol.2013.6746 pmid: 24173086
[12]	Glutsch V, Hamm H, Goebeler M. Zinc and skin: an update[J]. Journal der Deutschen Dermatologischen Gesellschaft, 2019, 17 (6):589-596. doi: 10.1111/ddg.13811 pmid: 30873720
[13]	Abendrot M, Płuciennik E, Felczak A, et al. Zinc(Ⅱ) complexes of amino acids as new active ingredients for anti-acne dermatological preparations[J]. International Journal of Molecular Sciences, 2021, 22 (4).
[14]	Rodríguez-Cotto R I, Ortiz-Martínez M G, Rivera-Ramírez E, et al. Particle pollution in Rio de Janeiro, Brazil: increase and decrease of pro-inflammatory cytokines IL-6 and IL-8 in human lung cells[J]. Environmental Pollution (Barking, Essex: 1987), 2014, 194: 112-120. doi: 10.1016/j.envpol.2014.07.010
[15]	Zouboulis C C, Jourdan E, Picardo M. Acne is an inflammatory disease and alterations of sebum composition initiate acne lesions[J]. Journal of the European Academy of Dermatology and Venereology, 2014, 28 (5):527-532. doi: 10.1111/jdv.12298 pmid: 24134468
[16]	Schneider M R, Paus R. Sebocytes, multifaceted epithelial cells: lipid production and holocrine secretion[J]. The International Journal of Biochemistry & Cell Biology, 2010, 42 (2):181-185. doi: 10.1016/j.biocel.2009.11.017
[17]	Barnard E, Li H. Shaping of cutaneous function by encounters with commensals[J]. The Journal of Physiology, 2017, 595 (2):437-450. doi: 10.1113/JP271638 pmid: 26988937
[18]	Belkaid Y, Segre J A. Dialogue between skin microbiota and immunity[J]. Science (New York, N.Y.), 2014, 346 (6212):954-959. doi: 10.1126/science.1260144
[19]	Akaza N, Akamatsu H, Numata S, et al. Microorganisms inhabiting follicular contents of facial acne are not only Propionibacterium but also Malassezia spp[J]. The Journal of Dermatology, 2016, 43 (8):906-911. doi: 10.1111/1346-8138.13245 pmid: 26705192
[20]	Cui R R, Li W, Shen Z, et al. Assessment of skin care behavior and cognition of patients with acne vulgaris in China[J]. Chinese Medical Journal, 2021, 134 (5):614-615. doi: 10.1097/CM9.0000000000001397
[21]	Meng B X, Jian-Ying S U. Detection of malassezia furfur in lesions on face of patients with acne and analysis of therapeutic effect of ketoconazole[J]. China Tropical Medicine, 2003, 3: 468-469.
[22]	Jia Y, Gan Y, He C, et al. The mechanism of skin lipids influencing skin status[J]. Journal of Dermatological Science, 2018, 89 (2):112-119. doi: S0923-1811(17)30979-9 pmid: 29174114
[23]	Cervantes J, Eber A E, Perper M, et al. The role of zinc in the treatment of acne: A review of the literature[J]. Dermatologic Therapy, 2018, 31 (1).
[24]	Chen H, Hossain M A, Kim J H, et al. Kahweol exerts skin moisturizing activities by upregulating STAT1 activity[J]. International Journal of Molecular Sciences, 2021, 22 (16).
[25]	Verdier-Sévrain S, Bonté F. Skin hydration: a review on its molecular mechanisms[J]. Journal of Cosmetic Dermatology, 2007, 6 (2):75-82. doi: 10.1111/j.1473-2165.2007.00300.x pmid: 17524122