中草药祛痘复方对痤疮致病菌和外泌酶的影响

doi:10.13218/j.cnki.csdc.2018.07.008

摘要/Abstract

摘要： 通过药敏试验和抑菌试验考察中草药祛痘复方对痤疮丙酸杆菌(Propionibacterium acnes,PA)、金黄色葡萄球菌(Staphylococcus aureus,SA)和表皮葡萄球菌(Staphylococcus epidermidis,SE)的抑菌功效,同时考察其对3种菌的抑菌时间动力学的影响。采用脂肪酶和蛋白酶水解底物形成的透明圈,研究中草药祛痘复方对PA释放脂肪酶和蛋白酶的影响。结果表明,中草药祛痘复方明显抑制了PA、SA和SE,并呈时间和剂量依赖性关系;中草药祛痘复方抑制脂肪酶和蛋白酶的作用主要是通过抑制PA而发挥作用。

关键词: 化妆品添加剂, 中草药祛痘复方, 痤疮丙酸杆菌, 金黄色葡萄球菌, 表皮葡萄球菌, 脂肪酶, 蛋白酶

Abstract: The antimicrobial efficacy of Chinese herbal anti-acne formula on Propionibacterium acnes (PA),Staphylococcus aureus (SA),and Staphylococcus epidermidis (SE) were investigated by susceptibility test and bacteriostatic test,and the bacteriostatic kinetics of it on these three bacterial were also examined.A transparent circle formed by lipase and protease hydrolysis substrate was used to study the effect of Chinese herbal anti-acne formula on the release of lipase and protease from PA.The results show that Chinese herbal anti-acne formula significantly inhibits PA,SA and SE in a time-and dose-dependent manner.The inhibitory effect of Chinese herbal anti-acne formula on lipase and protease mainly depends on inhibiting PA.

Key words: additives of cosmetics, Chinese herbal anti-acne formula, Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, lipase, protease

中图分类号:

TQ658

贺改英,陈迪,沈丽. 中草药祛痘复方对痤疮致病菌和外泌酶的影响[J]. 日用化学工业, 2018, 48(7): 403-407.

HE Gai-ying,CHEN Di,SHEN Li. The effect of Chinese herbal anti-acne formula on pathogenic bacteria and exocrine enzyme of acne vulgaris[J]. China Surfactant Detergent & Cosmetics, 2018, 48(7): 403-407.

参考文献

[1] Dunn L K,O′ Neill J L,Feldman S R.Acne in adolescents:Quality of life,self-esteem,mood and psychological disorders [J].Dermatology Online Journal,2011,17(1):1.
[2] UhLenhake E,Yentzer B A,Feldman S R,et al.Acne vulgaris and depression:a retrospective examination [J].Journal of Cosmetic Dermatology,2010,9(1):59-63.
[3] Zhu Lianhua,Jin Zhehu.The role of Propionibacterium acnes in the pathogenesis of acne [J].Chinese Journal of Aesthetic Medicine,2006,15(4):476-477.
[4] Lovekov Y,Havlíkov I,Yvona Lovekov A,et al.A microbiological approach to acne vulgaris [J].Biomedical Papers,2002,146(2):29-32.
[5] Jahns A C,Eilers H,Ganceviciene R,et al.Propionibacterium species and follicular keratinocyte activation in acneic and normal skin [J].British Journal of Dermatology,2015,172(4):981-987.
[6] Leeming,J P,Ingham E,Cunliffe W J.The microbial content and complement C3 cleaving capacity of comedones in acne vulgaris [J].Acta Dermato-Venereologica,1988,68(6):468-473.
[7] Vowels B R,Yang S,Leyden J J.Induction of proinflammatory cyto-
kines by a soluble factor of Propionibacterium acnes:implications for chronic inflammatory acne [J].Infection Immunity,1995,63(8):3158-3165.
[8] Hassanzadeh P,Bahmani M,Mehrabani D.Bacterial resistance to antibiotics in acne vulgaris:An in vitro study [J].Indian Journal of Dermatology,2008,53(3):122-124.
[9] Chen Xiaomin,Wu Lihui,Yang Zhihua.Clinical research on treating 66 cases of acne with red and blue light plus hedyotis diffusa [J].Clinical Journal of Chinese Medicine,2014,6(5):82-83.
[10] Liu Wenbin,Liang Qing,Wand Hui,et al.Experimental study on anti-acne mechanism of tanshinone cream [J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(14):212-216.
[11] Patil V, Bandivadekar A,Debjani D. Inhibition of Propionibacterium acnes lipase by extracts of Indian medicinal plants [J].International Journal of Cosmetic Science,2012,34(3):234-239.
[12] Wunnoo S,Saising J,Voravuthikunchai S P.Rhodomyrtone inhibits lipase production,biofilm formation,and disorganizes established biofilm in Propionibacterium acnes [J].Anaerobe,2017,43:61-68.
[13] Higaki S,Kitagawa T,Kagoura M,et al.Correlation between Propionibacterium acnes biotypes,lipase activity and rash degree in acne patients [J].The Journal of Dermatology,2000,27(8):519-522.
[14] Lee S E,J M,Jeong S K,et al.Protease-activated receptor-2 mediates the expression of inflammatory cytokines,antimicrobial peptides,and matrix metalloproteinases in keratinocytes in response to Propionibacterium acnes [J].Archives of Dermatological Research,2010,302(10):745-756.
[15] Higaki S.Lipase inhibitors for the treatment of acne [J].Journal of Molecular Catalysis B Enzymatic,2003,22:377-384.