百合花肽抗衰舒缓功效研究

doi:10.3969/j.issn.2097-2806.2025.11.012

摘要/Abstract

摘要： 该研究旨在探讨酶解发酵技术得到的兰州百合花肽（LLFPs）原料的抗衰老、舒缓作用。采用LC-MS/MS分析鉴定百合花肽的化学成分，通过荧光探针和酶联免疫吸附法、qRT-PCR、冰乙酸刺激斑马鱼模型、人体临床等研究手段，研究抗衰老、舒缓相关指标。结果表明，百合花肽含有535种短肽，其中分子量<1 000 Da占比76.09%；百合花肽可以降低MMP-1、ROS表达，在促进真皮成纤维细胞增殖及胶原蛋白分泌、调节CLOCK、BMAL1基因、对UVB诱导的角质细胞光氧化应激损伤、以及降低冰乙酸刺激斑马鱼总运动距离等方面具有显著作用；此外，该研究在人体临床方面验证了百合花肽的抗衰老、舒缓功效。基于体外和体内功效验证，揭示百合花肽具有抗衰舒缓作用，为其在化妆品中应用提供依据。

关键词: 百合花肽, 光损伤, 舒缓, 抗衰

Abstract:

The aim of this study was to investigate the anti-aging and soothing effects of Lanzhou lily flower peptides （LLFPs） generated via enzymatic hydrolysis and microbial fermentation. Comprehensive methodologies including LC-MS/MS analysis, fluorescent probes, ELISA, qRT-PCR, an acetic acid-induced zebrafish model, and human clinical trials were employed to evaluate the related biomarkers across multiple dimensions, alongside chemical component identification. Results demonstrate that LLFPs contain 535 short-chain peptides, with 76.09% exhibiting molecular weights <1 000 Da. The peptides significantly reduce UVB-induced MMP-1 and ROS expression in keratinocytes, promote dermal fibroblast proliferation and collagen secretion, modulate circadian clock genes （CLOCK, BMAL1）, alleviate UVB-induced photo-oxidative stress in keratinocytes, and decrease total movement distance in acetic acid-stimulated zebrafish. Clinical trials further validate the anti-aging and soothing efficacy of LLFPs in human subjects. Through integrated in vitro and in vivo verification, this research reveals the dual anti-aging and soothing efficacy of LLFPs, providing a scientific foundation for their application in cosmetic formulations.

Key words: lily flower peptide, photo-damage, soothing, anti-aging

中图分类号:

TQ658

李配配, 徐晓明, 张强, 方杨杨, 李慧良. 百合花肽抗衰舒缓功效研究[J]. 日用化学工业（中英文）, 2025, 55(11): 1460-1467.

Peipei Li, Xiaoming Xu, Qiang Zhang, Yangyang Fang, Huiliang Li. Study on anti-aging and soothing effects of lily flower peptides[J]. China Surfactant Detergent & Cosmetics, 2025, 55(11): 1460-1467.

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引物名称	序列
BMAL1	F：5'-GAAGACAACGAACCAGACAATGAG-3'
BMAL1	R：5'-GCGTGCCGAGAAACATATTCC-3'
CLOCK	F：5'-GCCAGTGAAGCTGGGTTTTC-3'
CLOCK	R：5'-CAGCAGCTTTGCAGGAACAA-3'

肽段注释序列	该肽段归属的主蛋白名称	肽段理论分子量/Da	丰度值	保留时间/min
[D]. QLSDLQ.[R]	A0A023ZT32	703.362 1	788 467 264	18.249 3
[V]. EIVDDIHAAIDHIH.[Q]	A0A1B1HXB5	1 597.796 9	782 008 896	7.429 5
[A]. DTGGFLE.[V]	A0A097PNF8	738.330 46	761 825 088	27.434 5
[M]. IIAVPT.[G]	A4ZEV3	613.391 94	216 385 088	38.028 5
[N]. FEGSI.[P]	A0A248SMI3	552.266 4	210 446 960	35.720 6
[L]. LQDIVPT.[F]	A0A894TLA1	785.440 35	208 462 585	60.380 6
[C]. IQGLPE.[G]	W6HY22	656.361 37	194 289 072	25.711 4
[A]. VEEAGGP.[K]	A0A089VKG4	658.304 25	193 021 456	22.795 8
[P]. EDIAPN.[K]	A0A2L0E720	658.304 25	193 021 456	22.814 7
[N]. LTDDDV.[V]	C7SJ62	677.298 83	169 214 496	16.657 5

指标		D0	D14	D28
皱纹参数（眼角鱼尾纹皱纹面积/mm²）	均值	15.47±8.05	16.71±8.63	13.33±6.94
皱纹参数（眼角鱼尾纹皱纹面积/mm²）	变化率		8.00%	-13.81%
R2	均值	0.60±0.112 6	0.60±0.823	0.61±0.815
R2	变化率		0.66%	2.83%
F4	均值	10.93±2.22	10.81±1.92	9.78±2.35^**
F4	变化率		-1.10%	-10.51%
水分含量/c.u.	均值	63.22±11.38	75.39±9.68	79.28±8.22
水分含量/c.u.	变化率		19.26%^*	25.41%^**
a^*值	均值	32.62±2.85	28.70±4.41^*	29.51±1.57^**
a^*值	变化率		-12.01%	-9.52%
经皮水分流失/（g/ （cm²·h））	均值	28.34±5.44	23.22±5.93	22.43±5.26
经皮水分流失/（g/ （cm²·h））	变化率		-18.04%	-20.84%^*