重组Ⅲ型胶原蛋白对皮肤光老化的改善作用研究

doi:10.3969/j.issn.2097-2806.2025.11.011

摘要/Abstract

摘要：

为探究重组Ⅲ型胶原蛋白（RHC-Ⅲ）对皮肤光老化的改善作用。采用原代皮肤细胞和离体皮肤组织模型，通过结晶紫染色法、酶联免疫吸附测定法和免疫荧光法等检测皮肤中光老化标志物的变化情况。细胞实验结果显示，与空白对照组相比，RHC-Ⅲ可增加NHDFs细胞的粘附能力；与模型对照组相比，RHC-Ⅲ可降低细胞内ROS水平和MMPs分泌量并提高CAT酶活力；离体皮肤组织模型实验结果显示，与模型对照组相比，RHC-Ⅲ可提高COL-Ⅳ、COL-Ⅶ、COL-ⅩⅦ、整合素α6亚单位、COL-Ⅰ、COL-Ⅲ和弹性蛋白的含量。以上结果表明，RHC-Ⅲ改善皮肤光老化的机制可能与促进成纤维细胞粘附，减少皮肤细胞氧化应激，减少细胞外基质（ECM）的降解，促进DEJ结构相关蛋白的表达，提高真皮层细胞外基质关键蛋白的含量有关。

关键词: 重组Ⅲ型胶原蛋白, 光老化, 离体皮肤, 氧化应激, 真表皮连接

Abstract:

The aim of this study was to investigate the mechanism of recombinant human collagen Ⅲ （RHC-Ⅲ） on anti-skin photoaging. The blank control group, model control group, positive control group and sample groups were set up in the experiment. Primary human skin cells and ex vivo human skin tissue model were used to detect the changes of photoaging markers in dermal-epidermal junction （DEJ） and dermis by crystal violet staining, enzyme-linked immunosorbent assay （ELISA） and immunofluorescence. The results of the cell experiments show that compared with the blank control group, RHC-Ⅲ significantly increases the adhesion ability of normal human dermal fibroblasts （NHDFs）. Compared with the model control group, RHC-Ⅲ significantly decreases the level of ROS, MMPs secretion and increases the activity of CAT. In ex vivo human skin tissue model experiments, compared with the model control group, RHC-Ⅲ significantly increases the contents of COL-Ⅳ, COL-Ⅶ, COL-ⅩⅦ, integrin α6 subunit, COL-Ⅰ, COL-Ⅲ and elastin. The results indicate that the mechanism of RHC-Ⅲ improving skin photoaging is related to promoting NHDFs adhesion, reducing oxidative stress and the degradation of extracellular matrix, improving the expression of DEJ structure-related proteins, and increasing the synthesis of key extracellular matrix proteins in dermis.

Key words: recombinant human collagen Ⅲ, photoaging, ex vivo human skin model, oxidative stress, dermal-epidermal junction

中图分类号:

TQ658

刘菲, 王倩, 刘慧, 杨素珍, 韩婷婷, 武涛. 重组Ⅲ型胶原蛋白对皮肤光老化的改善作用研究[J]. 日用化学工业（中英文）, 2025, 55(11): 1452-1559.

Fei Liu, Qian Wang, Hui Liu, Suzhen Yang, Tingting Han, Tao Wu. Study on the effect of recombinant human collagen Ⅲ on anti-skin photoaging[J]. China Surfactant Detergent & Cosmetics, 2025, 55(11): 1452-1559.

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ρ（RHC-Ⅲ）/ （mg/mL）	Cell viability/%
ρ（RHC-Ⅲ）/ （mg/mL）	NHEKs	NHDFs
0	100.00±1.99	100.00±3.89
0.08	96.05±4.47	101.30±4.92
0.16	96.36±6.26	101.04±2.61
0.31	95.60±1.74	99.06±3.77
0.63	95.92±3.16	99.69±3.34
1.25	94.26±4.34	99.69±4.86
2.50	93.49±3.47	97.29±3.38
5.00	90.88±2.55	96.30±4.02
10.00	72.19±3.18	96.04±6.32