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日用化学工业（中英文）

日用化学工业（中英文） ›› 2024, Vol. 54 ›› Issue (9): 1069-1077.doi: 10.3969/j.issn.2097-2806.2024.09.007

• 开发与应用 • 上一篇    下一篇

构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用及机制研究

赖梓漩1,宋雨轩1,段雪伟2,刘诗芸1,刘冰1,张敏君2,杨慧文1,*()   

  1. 1.广东药科大学 药学院，广东 广州 510006
    2.广东药科大学 护理学院，广东 广州 510006
  • 收稿日期:2023-10-09 修回日期:2024-08-20 出版日期:2024-09-22 发布日期:2024-09-26
  • 基金资助:
    国家级大学生创新创业训练计划项目(202310573012)

The protective effect and mechanism of crude polysaccharide from Broussonetia papyrifera flower on UV-induced skin photodamage

Zixuan Lai1,Yuxuan Song1,Xuewei Duan2,Shiyun Liu1,Bing Liu1,Minjun Zhang2,Huiwen Yang1,*()   

  1. 1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
    2. School of Nursing, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China
  • Received:2023-10-09 Revised:2024-08-20 Online:2024-09-22 Published:2024-09-26
  • Contact: * E-mail: halleyyang@163.com.

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摘要：

研究构树花粗多糖对紫外线诱导小鼠皮肤光损伤的保护作用，并探讨其作用机制。采用雌性昆明鼠建立长波紫外线（UVA）诱导小鼠皮肤光损伤模型，并对小鼠背部皮肤进行评分。采用苏木素-伊红（HE）、Masson染色考察小鼠皮肤组织病理学变化、皮肤厚度和胶原纤维的变化；考察皮肤组织Hyp、抗氧化酶SOD、GSH-Px、CAT和MDA，TNF-α、IL-1β、IL-6的含量，MMPs、Nrf2、HO-1和NF-κB通路相关蛋白的表达。结果显示，构树花粗多糖凝胶可不同程度改善皮肤粗糙溃烂现象，抑制皮肤增厚，提高真皮层胶原含量，使胶原纤维排列趋于紧密；提高皮肤组织中SOD、GSH-Px和CAT活力，降低MDA含量（P<0.05）；降低皮肤促炎因子TNF-α、IL-1β和IL-6含量（P<0.05）；MMP-1和MMP-3含量明显降低（P<0.05），Nrf2蛋白和HO-1蛋白表达显著升高（P<0.01），p-p65/p65蛋白表达比例显著降低（P<0.05）。可见，构树花粗多糖对紫外线致小鼠皮肤光损伤具有显著的保护作用，其机制可能与构树花粗多糖激活Nrf2通路、增强SOD等抗氧化酶活性，抑制NF-κB通路有关。

关键词: 构树花粗多糖, 皮肤光损伤, 氧化应激, 炎症因子, Nrf2通路

Abstract:

To investigate the protective effect of crude polysaccharide from Broussonetia papyrifera flower on skin photodamage, a total of 56 female Kunming mice were randomly divided into 7 groups: control group, model group, matrix gel group, vitamin A group, and crude polysaccharide low-dose, medium-dose and high-dose groups. The exposed back skin of the mice was irradiated by UVA to induce skin photodamage model, and the drugs were applied to the skin 1 hour before irradiation. After 8 weeks of continuous irradiation, the back skin was scored. The effects of crude polysaccharide from Broussonetia papyrifera flower on the back skin morphology, skin thickness and collagen fibers were investigated by hematoxylin-eosin and Masson staining. And the contents of skin hydroxyproline, SOD, GSH-Px, CAT, MDA, TNF-α, IL-1β, and IL-6, and the protein expressions of MMP-1/3, Nrf2, HO-1 and NF-κB pathway were also examined. Pre-treated skin with different dose of crude polysaccharide gel 1 hour before UVA irradiation can improve roughness and ulceration of the back skin to different degrees, ameliorate skin thickening, and dermal collagen damage. At the same time, the activities of SOD, GSH-Px and CAT in skin tissue are increased, and the content of MDA is decreased （P<0.05）. The contents of pro-inflammatory cytokines, TNF-α, IL-1β and IL-6, are decreased （P<0.05）. The contents of MMP-1 and MMP-3 are decreased significantly （P<0.05）, the expression of Nrf2 and HO-1 protein are increased （P<0.01）, and p-p65/p65 protein are decreased significantly （P<0.05） in the mouse back skin by pre-treatment with crude polysaccharide gel. The crude polysaccharide from Broussonetia papyrifera flower has a strong antioxidant activity in vitro, and a protective effect on UVA-induced mice skin photodamage in vivo. The mechanism may be related to the activation of Nrf2 and inhibition of NF-κB signaling pathway, leading to the regulation of antioxidative enzyme SOD and improvement of inflammatory response.

Key words: crude polysaccharide of Broussonetia papyrifera flower, skin photodamage, oxidative stress, proinflammatory cytokine, Nrf2 pathway

中图分类号: 

  • TQ658