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日用化学工业（中英文）

日用化学工业 ›› 2019, Vol. 49 ›› Issue (8): 531-536.doi: 10.3969/j.issn.1001-1803.2019.08.008

• 开发与应用 • 上一篇    下一篇

白果内酯和银杏内酯B对过氧化氢诱导人角质形成细胞氧化损伤的保护作用

熊丽丹1,2,唐洁1,2,李利1,2,3()   

  1. 1. 四川大学华西医院 化妆品评价中心,四川 成都 610041
    2. 四川省化妆品工程技术研究中心,四川 成都 610041
    3. 四川大学华西医院 皮肤科,四川 成都 610041
  • 收稿日期:2018-12-05 修回日期:2019-08-05 出版日期:2019-08-22 发布日期:2019-08-26
  • 通讯作者: 李利
  • 作者简介:熊丽丹,女,四川成都人,助理研究员,电话：13882285741,E-mail： 1585244575@qq.com。
  • 基金资助:
    四川大学华西医院学科卓越发展1·3·5工程项目;四川省科技计划资助项目(2018SZ0104);四川省科技计划资助项目(2017SZ0070)

Protective effects of bilobalide and ginkgolides B on oxidative damage in human keratinocytes induced by H2O2

XIONG Li-dan1,2,TANG Jie1,2,LI Li1,2,3()   

  1. 1. Cosmetics Safety and Efficacy Evaluation Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
    2. Sichuan Engineering Technology Research Center of Cosmetic, Chengdu, Sichuan 610041, China
    3. Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
  • Received:2018-12-05 Revised:2019-08-05 Online:2019-08-22 Published:2019-08-26
  • Contact: Li LI

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摘要：

通过CCK-8法测定细胞存活率,WST染色方法测定超氧化物歧化酶（SOD）活性,DTNB测定谷胱甘肽（GSH）活性,脂质氧化试剂盒检测丙二醛（MDA）含量,DCFH-DA法测定活性氧（ROS）含量,ELISA法测定炎症因子TNF-α、IL-1β和IL-6含量水平,研究了白果内酯和银杏内酯B对过氧化氢（H2O2）诱导人角质形成细胞（HaCaT细胞）的抗氧化损伤和抑制炎症因子的影响,同时利用药物数据库预测目标靶点,研究白果内酯和银杏内酯B发挥功效的机制。结果表明,白果内酯与银杏内酯B能减少H2O2诱导的SOD、谷胱甘肽过氧化物酶（GSH-Px）活性降低,抑制MDA释放增加,减少ROS的产量,减少炎症因子TNF-α、IL-1β、IL-6的生成;综合网络分析结果显示白果内酯和银杏内酯B抗炎活性呈现多分子、多靶点和多通路特点。

关键词: 白果内酯, 银杏内酯B, 炎症因子, 活性氧, 体外实验

Abstract:

Different concentrations of bilobalide and ginkgolides B were added to H2O2-stimulated HaCaT model. Cell viability was determined by CCK-8 method, superoxide dismutase（SOD）activity was determined by WST staining method, glutathione（GSH）activity was measured by DTNB, malondialdehyde（MDA）content was detected by lipid oxidation kit. The content of ROS was investigated via DCFH-DA method. And ELISA assays were adopted to investigate the production of TNF-α, IL-1β, and IL-6. To better understand the mechanism of the efficacy of bilobalide and ginkgolides B, TCM database（IUPHAR/BPS Guide to Pharmacology and String）was used to predict common targets of drugs. The results show that bilobalide and ginkgolides B can increase the SOD and GSH-Px activity, decrease MDA and ROS content induced by H2O2, and reduce the production of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 in H2O2-induced HaCaT cells. The drug discovery database shows that the anti-inflammatory effect of bilobalide and ginkgolides B has the characteristics as multi-molecular, multi-target and multi-pathway.

Key words: bilobalide, ginkgolides B, inflammatory factor, ROS, in vitro test

中图分类号: 

  • TQ658