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日用化学工业 ›› 2019, Vol. 49 ›› Issue (9): 585-589.doi: 10.3969/j.issn.1001-1803.2019.09.005

• 开发与应用 • 上一篇    下一篇

侧柏叶提取物对SOD酶活力修复作用及抗炎功效

唐洁1,2,杨海延4,李利1,2,3,马冬月4,董婷婷4,熊丽丹1,2()   

  1. 1. 四川大学 华西医院 化妆品评价中心,四川 成都 610041
    2. 四川省化妆品工程技术中心,四川 成都 610041
    3. 四川大学 华西医院皮肤科,四川 成都 610041
    4. 上海辉文生物技术股份有限公司,上海 200120
  • 收稿日期:2018-12-07 修回日期:2019-09-03 出版日期:2019-09-22 发布日期:2019-09-19
  • 通讯作者: 熊丽丹
  • 作者简介:唐 洁,女,四川人,助理研究员,硕士,电话：18428397228,E-mail： tj_hxyy@163.com。
  • 基金资助:
    四川大学华西医院学科卓越发展1·3·5工程项目资助;四川省科技计划(2018SZ0104);四川省科技计划(2017SZ0070)

Repair effect on SOD enzyme and anti-inflammatory activity of the platycladus orientalis extract

TANG Jie1,2,YANG Hai-yan4,LI Li1,2,3,MA Dong-yue4,DONG Ting-ting4,XIONG Li-dan1,2()   

  1. 1. Cosmetics Safety and Efficacy Evaluation Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
    2. Sichuan Engineering Technology Research Center of Cosmetic, Chengdu, Sichuan 610041, China
    3. Department of Dermatology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
    4. Shanghai Huiwen Biotechnology Co., Ltd., Shanghai 200120, China
  • Received:2018-12-07 Revised:2019-09-03 Online:2019-09-22 Published:2019-09-19
  • Contact: Li-dan XIONG

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摘要：

为探索侧柏叶提取物对细胞损伤的修复作用及抗炎功效,采用SDS刺激HaCaT细胞和LPS诱导RAW264.7细胞构建炎症模型。随后加入侧柏叶提取物,观察其对SDS致HaCaT细胞损伤后的SOD酶活力修复作用及对RAW264.7细胞上清中炎症因子分泌的抑制作用。结果表明,SDS刺激HaCaT细胞后,能够对细胞造成一定损伤,并致使细胞内SOD酶活力降低,随后加入侧柏叶提取物,其对SOD酶活力有修复作用,最高修复率为85.6%,表明侧柏叶提取物具有修复SOD酶活力功效。同时LPS诱导RAW264.7细胞,构建炎症模型成功后,随后加入侧柏叶提取物,其对RAW264.7细胞上清NO含量具有降低作用,同时,能够抑制IL-6,IL-1β和TNF-α炎症因子的释放,表明侧柏叶提取物具有抗炎功效。

关键词: 侧柏叶提取物, SOD酶, 细胞损伤修复, 炎症因子, 抗炎

Abstract:

In order to explore the repair of cell damage and anti-inflammatory effects of the platycladus orientalis extract（CBY）, SDS was used to stimulate HaCaT cells to establish an injury model and LPS was used to induce RAW264.7 cells to construct an inflammatory model. After that, CBY was added to repair the SOD activity of damaged HaCaT cells and decrease the inflammatory factors of RAW264.7. The results show that HaCaT cells are damaged to a certain extent after being stimulated by SDS, and the enzyme activity of intracellular SOD is decreased. The addition of platycladus orientalis extract has repair effect on the enzyme activity of SOD with the highest repair rate of 85.6%. Besides, there is high expression of inflammatory factors（NO, IL-6, IL-1β, TNF-α）in RAW264.7 cells after the addition of LPS, while all the factors are decreased with the addition of platycladus orientalis extract, which suggested that the platycladus orientalis extract has anti-inflammatory effect.

Key words: platycladus orientalis extract, SOD enzyme, cell damage repair, inflammatory factor, anti-inflammatory

中图分类号: 

  • TQ658