百子莲脱水素蛋白对人皮肤细胞损伤的保护作用

doi:10.3969/j.issn.1001-1803.2020.02.002

摘要/Abstract

摘要：

应用角质形成细胞（HaCaT）与成纤维细胞（NHSF）建立脱水、光老化及氧化应激模型评价百子莲Y₂SK₂与SK₃型脱水素蛋白对人皮肤细胞在不同胁迫条件下的保护功能。结果表明：0.01~10 μmol/L为两种脱水素的安全作用浓度。光老化与氧化应激模型会造成人皮肤细胞发生氧化损伤与细胞凋亡。10 μmol/L ApSK₃可将皮肤细胞超低温冻存后细胞活性提升57%~91%。ApSK₃可明显改善皮肤细胞在脱水处理中的胁迫损伤,两种脱水素在光老化与氧化应激模型中对皮肤细胞均有抗氧化保护作用,可显著降低细胞中MDA含量约10%~36%,提升HaCaT细胞的SOD酶活性;ApSK₃可有效抑制皮肤细胞在氧化胁迫下的casepase-3与casepase-8的酶活性,降低细胞程序性死亡事件发生。该研究发现脱水素蛋白在胁迫伤害中对人皮肤细胞具有保护作用,其机制可能与增强细胞抗氧化性以及参与细胞凋亡过程有关。

关键词: 脱水素蛋白, 角质细胞, 成纤维细胞, 氧化应激, 细胞凋亡

Abstract:

Normal human skin fibroblast （NHSF） cells and HaCaT cells were applied to establish evaluation models of dehydration, light-aging and oxidative stress. Based on the models, the protective effects of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox （ApY₂SK₂ and ApSK₃） on human skin cells were studied. The results showed that, the safe concentration of dehydrins was 0.01-10 μmol/L. Light-aging and oxidative stress could cause oxidative damage and apoptosis of human skin cells. 10 μmol/L ApSK₃ could increase the activity of skin cells by 57%-91% after cryopreservation. ApSK₃ could significantly improve the activity of skin cells under dehydration. Both the two dehydrins provided anti-oxidative protection for skin cells in the models of light-aging and oxidative stress, in which the level of malondialdehyde was decreased by 10%-36% and the activity of SOD was significantly increased for HaCaT cells. The activity of casepase-3 and casepase-8 was decreased by ApSK₃according to the oxidative stress model, and the occurrence of programmed cell death was reduced. The research showed that, Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox could protect human skin cells against stress damage, the mechanism of which could possibly be related to the enhanced cell anti-oxidation and their participation in the process of cell apoptosis.

Key words: dehydrin, HaCaT cell, NHSF cell, oxidative stress, cell apoptosis

中图分类号:

TQ658

吕可,李婧,张荻. 百子莲脱水素蛋白对人皮肤细胞损伤的保护作用[J]. 日用化学工业, 2020, 50(2): 76-85.

LV Ke,LI Jing,ZHANG Di. Protection of human skin cells against stress damage by use of Y₂SK₂ and SK₃ type dehydrins from Agapanthus praecox[J]. China Surfactant Detergent & Cosmetics, 2020, 50(2): 76-85.

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组别	蛋白浓度/（μmol·L^-1）	HaCaT细胞增殖率/%			NHSF细胞增殖率/%
不添加脱水素空白组	0	100			100
添加ApY₂SK₂实验组	0.01	96.65	±	9.56	96.07	±	6.78
	0.05	103.17	±	10.64	95.24	±	11.22
	0.1	114.79	±	11.33	111.87	±	7.50
	0.5	124.47	±	12.64^*	111.49	±	7.82
	1	127.99	±	9.54^*	118.14	±	10.97^*
	5	133.45	±	11.11^*	128.72	±	10.24^*
	10	135.56	±	16.90^*	116.70	±	3.96^*
添加ApSK₃实验组	0.01	97.90	±	9.96	97.12	±	8.75
	0.05	110.23	±	6.17^*	102.34	±	16.90^*
	0.1	111.84	±	10.07^*	117.31	±	14.17^*
	0.5	124.78	±	8.52^*	118.33	±	14.96^*
	1	136.78	±	7.37^*	120.74	±	10.25^*
	5	126.11	±	8.94^*	132.24	±	15.23^*
	10	120.96	±	9.85^*	127.85	±	22.26