紫茉莉花提取物促进胶原蛋白功能化的功效研究

doi:10.3969/j.issn.1001-1803.2019.08.007

摘要/Abstract

摘要：

采用人真皮成纤维细胞体外培养,利用酶联免疫吸附测定方法,结合细胞活性检测,测定对胶原蛋白功能化有重要作用的前胶原C端蛋白酶（BMP-1）在成纤维细胞培养过程中的分泌量,通过考察较佳测定时间、选择稳定的阳性对照品,建立和优化BMP-1的细胞测试模型。结果显示,紫茉莉花提取物质量浓度为0.05 g/L时,BMP-1含量是对照组的150.4%±8.5%（P<0.001）。紫茉莉花提取物可以显著促进BMP-1的产生,具有促进胶原蛋白功能化的护肤功效。

关键词: 护肤, 前胶原C端蛋白酶, 胶原蛋白功能化, 人真皮成纤维细胞, 紫茉莉

Abstract:

Human dermal fibroblasts were cultured in vitro, and enzyme-linked immunosorbent assay（ELISA）combined with cell activity assay was used to determine the secretion of BMP-1, which plays an important role in collagen functionalization. The measurement timepoint and positive control of the assay model were optimized. Based on the standardized assay model, the efficacy of Mirabilis jalapa flower extract on BMP-1 synthesis was evaluated. The results show that when treated with Mirabilis jalapa flower extract at 0.05 g/L, the relative BMP-1 content of the fibroblasts is 150.4%±8.5% compared with the control group（P<0.001）. Therefore, the Mirabilis jalapa flower extract can significantly promote the production of BMP-1 from dermal fibroblasts, and has the great potential in skincare effect of promoting collagen functionalization.

Key words: skin care, procollagen C-proteinase, collagen functionalization, human dermal fibroblast, Mirabilis jalapa

中图分类号:

TQ658

李慧,蒋丹丹,DOS SANTOS Morgan,章漳,蒋耀权. 紫茉莉花提取物促进胶原蛋白功能化的功效研究[J]. 日用化学工业, 2019, 49(8): 526-530.

LI Hui,JIANG Dan-dan,DOS SANTOS Morgan,ZHANG Zhang,JIANG Yao-quan. Efficacy evaluation of Mirabilis jalapa flower extract on promoting collagen functionalization[J]. China Surfactant Detergent & Cosmetics, 2019, 49(8): 526-530.

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参考文献 14

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t/h	OD值	CV/%
8	0.19	9.1
12	0.37	5.1
18	0.81	7.9
24	1.17	7.7
30	1.55	9.9

实验批次	VC（50 mmol/L）组	TNF-α（10 μg/L）组
第1批	108.5%±14.0%	129.2%±13.5%^*
第2批	88.1%±9.9%^**	141.8%±10.8%^**
第3批	87.7%±22.3%^*	142.9%±5.2%^**

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