茶多酚的经皮渗透特征及其对UVA致HSF氧化损伤的保护作用

doi:10.3969/j.issn.2097-2806.2023.09.006

摘要/Abstract

摘要：

研究了茶多酚的经皮渗透特征，探究茶多酚对长波紫外线（UVA）诱导的人皮肤成纤维细胞氧化损伤的保护作用机理。采用Franz扩散池，以大鼠皮肤为屏障，建立了完善的检测方法，用高效液相色谱检测茶多酚中儿茶素、表儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯的经皮渗透指标；通过建立UVA损伤HSF细胞模型，探究了茶多酚对细胞活性氧含量、丙二醛水平、超氧化物歧化酶和谷胱甘肽过氧化物酶活力的影响。结果表明该检测方法具有较好的专属性、精密度和加样回收率，且茶多酚中的儿茶素、表儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯4种物质均可较好地作用于皮肤，其中表儿茶素在透皮释放方面具有明显优势。茶多酚通过降低UVA辐照后HSF细胞中的ROS含量和MDA水平，可提高细胞内SOD和GSH-Px活力，对UVA诱导的HSF细胞氧化损伤具有良好的保护作用。

关键词: 茶多酚, 经皮渗透, 长波紫外线辐射, 人皮肤成纤维细胞, 氧化损伤

Abstract:

The aim was to study the transdermal penetration characteristics of tea polyphenols and explore the protective effect of tea polyphenols on UVA-induced oxidative damage in human skin fibroblasts （HSF）. Using the Franz diffusion cell with rat skin as a barrier, a well-performed detection method was established to detect the percutaneous penetration indexes of catechin, epicatechin, epicatechin gallate and epigallocatechin gallate in tea polyphenols by high performance liquid chromatography （HPLC）. The model of HSF damaged by UVA was established to explore the effect of tea polyphenols on the content of reactive oxygen species （ROS）, the level of malondialdehyde （MDA）, the enzymatic activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in cells. The results show that the detection method has good specificity, precision and average recovery. The transdermal permeability of catechin and epicatechin increase gradually with the increase of time, and the infiltration rate of epicatechin is greater than that of catechin. However, the transdermal permeability of epicatechin galate and epigallocatechin galate can not be detected in the 24 h experiment, but the two substances remain more in the skin, indicating that the four substances in tea polyphenols can be applied to the skin well. Besides, tea polyphenols have significant protective effect on UVA-induced oxidative damage in HSF by reducing the content of ROS and the level of MDA, increasing the enzymatic activity of SOD and GSH-Px.

Key words: tea polyphenols, percutaneous penetration, ultraviolet A radiation, human skin fibroblasts, oxidative damage

中图分类号:

TQ658

沈轶昕, 唐礼荣, 张辉. 茶多酚的经皮渗透特征及其对UVA致HSF氧化损伤的保护作用[J]. 日用化学工业（中英文）, 2023, 53(9): 1035-1043.

Shen Yixin, Tang Lirong, Zhang Hui. Percutaneous permeation characteristics of tea polyphenols and its protective effect on human skin fibroblasts under the oxidative damage of ultraviolet A[J]. China Surfactant Detergent & Cosmetics, 2023, 53(9): 1035-1043.

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对照品	回归方程	r	线性范围/ （μg/mL）
儿茶素	y=6 498.2x+1 122.9	0.999 9	1~500
表儿茶素	y=7 213.9x-237.09	0.999 9	1~500
表儿茶素没食子酸酯	y=16 399x-2 703.4	0.999 9	1~500
表没食子儿茶素没食子酸酯	y=11 322x-869.78	0.999 9	1~500

组分	仪器精密度/%	样品稳定性/%	加样回收率/%
儿茶素	0.97	0.45	0.32
表儿茶素	0.77	2.63	0.53
表儿茶素没食子酸酯	0.42	0.48	0.27
表没食子儿茶素没食子酸酯	0.67	12.22	0.39

组分	稳态渗透速率方程	渗透速率/ （μg/ （cm²·h））	时滞/h
儿茶素	y=1.406x-10.88	1.406	7.74
表儿茶素	y=2.614 1x-19.352	2.614 1	7.40
表儿茶素没食子酸酯	—	—	—
表没食子儿茶素没食子酸酯	—	—	—

组分	扩散池残留量/ （μg/cm²）	皮内滞留量/ （μg/cm²）	经皮累积渗透量/ （μg/cm²）
儿茶素	54.58±21.07	12.35±5.20	22.80±5.96
表儿茶素	80.37±29.43	16.99±6.25	42.98±8.40
表儿茶素没食子酸酯	80.24±44.92	94.82±33.64	0.00±0.00
表没食子儿茶素没食子酸酯	215.62±134.63	233.54±89.92	0.00±0.00