神经酰胺脂质体对光损伤的修护及机械损伤的修复功效研究

doi:10.3969/j.issn.2097-2806.2025.07.011

摘要/Abstract

摘要： 通过高压均质微射流技术制备了水溶性神经酰胺NP脂质体（CNPL），首先研究了CNPL理化性质和驻留性；再用UVB损伤的3D表皮皮肤模型研究CNPL的修护机理及功效；最后采用斑马鱼胚胎尾鳍切除模型研究了CNPL对机械损伤的修复效果。实验表明：制备得到的CNPL透明度好，10.00%质量分数以内的CNPL水溶液透光率与水接近；平均粒径22.25 nm （PDI=0.241），pH范围6.70~6.90；经皮驻留实验发现，CNPL中的神经酰胺NP的驻留量是未包裹的神经酰胺NP的3.8倍；3.00%体积分数的CNPL对UVB光损伤后的3D表皮皮肤模型中丝聚蛋白（FLG）和紧密连接蛋白1 （Claudin-1）的提升率分别为128.21%和73.68%，5.00%体积分数的CNPL对损伤模型中FLG和Claudin-1的提升率分别为194.87%和100.00%；质量浓度0.01和0.03 g/L的CNPL对斑马鱼胚胎尾鳍修复促进率分别为3.05%和8.60%。结果表明：CNPL具有很好的水溶性、稳定性、透光性、更高的透皮驻留性和生物利用度，对3D表皮皮肤模型中的光损伤具有一定的修护效果，也对斑马鱼尾鳍的机械损伤具有一定的修复效果，CNPL在配方应用中更加的便捷，高效，这有助于神经酰胺NP更广泛的应用。

关键词: 神经酰胺脂质体, 修护, 光损伤, 3D表皮皮肤模型, 斑马鱼胚胎尾鳍修复

Abstract:

In this study, water-soluble ceramide NP liposomes （CNPL） were prepared using high-pressure homogeneous microfluidization technology, and the physical and chemical properties of CNPL were thoroughly investigated. The repair mechanism and efficacy of CNPL were assessed utilizing a UVB-injured 3D epidermal skin model, alongside an examination of the repair effects on physical injury through the caudal fin excision model in zebrafish embryos. The experimental results indicate that CNPL has high transparency and stability, and the transmittance of CNPL aqueous solution within 10.00% mass fraction is close to that of the water. The average particle size is 22.25 nm （PDI=0.241）, and the pH range is 6.70-6.90. The permeability increases 3.8 times after 12 h. The improvement rates of FLG and Claudin-1 in 3.00% volume fraction CNPL are 128.21% and 73.68%, respectively. While the improvement rates of FLG and Claudin-1 in 5.00% volume fraction CNPL are 194.87% and 100.00%, respectively. The higher the concentration of CNPL, the stronger the improvement effect of FLG and Claudin-1, with 0.01 g/L and 0.03 g/L CNPL promoting the repair of zebrafish embryonic caudal fin by 3.05% and 8.60%, respectively. Overall, the findings suggest that CNPL possesses excellent water solubility, stability, transmittance, as well as superior transdermal absorption efficiency and bioavailability characteristics. It demonstrates significant reparative effects against UVB-induced light damage as well as physical injuries, thereby aiding in skin barrier restoration, delaying photoaging processes, promoting wound healing efforts, and presenting itself as an advantageous component for broader applications within cosmetic formulations.

Key words: ceramide liposomes, repair, light injury, 3D epidermal skin model, caudal fin repair of zebrafish embryos

中图分类号:

TQ658

刘敏, 叶磊, 蒋玲玲, 郑久炎. 神经酰胺脂质体对光损伤的修护及机械损伤的修复功效研究[J]. 日用化学工业（中英文）, 2025, 55(7): 902-908.

Min Liu, Lei Ye, Lingling Jiang, Jiuyan Zheng. Study on the repairing effect of ceramide liposomes on light injury and physical injury of skin[J]. China Surfactant Detergent & Cosmetics, 2025, 55(7): 902-908.

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