雪菊与芦荟醇提物对UVB导致的小鼠皮肤光损伤的保护作用

doi:10.3969/j.issn.2097-2806.2024.11.008

摘要/Abstract

摘要：

雪菊和芦荟在保护皮肤光损伤方面有极大的潜力。文章研究了雪菊和芦荟的乙醇提取物对户外紫外线（Ultraviolet radiation B，UVB）诱导的小鼠皮肤光损伤的保护作用。实验将小鼠分为正常对照组（NC组）、模型对照组（MC组）、雪菊醇提物组（SCAE组）、芦荟醇提物组（AVAE组）、雪菊芦荟醇提物组（SCAE+AVAE组）。通过对受损皮肤中抗氧化指标、羟脯氨酸（Hydroxyproline，HYP）含量和小鼠血清相关炎症因子的测定，初步揭示预防小鼠皮肤光损伤的作用机制。结果表明，与MC组相比，三个干预组对UVB诱导的小鼠皮肤外观均有明显改善，通过苏木精-伊红染色法（Hematoxylin-eosinstaining，HE）得出，SCAE+AVAE组和SCAE组比AVAE组效果更好，小鼠皮肤更加健康有弹性。三个干预组也可降低UVB诱导小鼠皮肤的氧化应激损伤，SCAE+AVAE组丙二醛（Malondialdehyde，MDA）含量仅为（28.18±4.25） nmol/mgprot，显著低于其他组；SCAE+AVAE组三种抗氧化酶：过氧化氢酶（Catalase，CAT）、超氧化物歧化酶（Superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GSH-Px）活性也显著升高。SCAE+AVAE组较单独醇提物组效果更佳，HYP含量达到了（4.12±0.18） μg/mg。三个干预组均可下调炎症因子含量，抑制炎症反应，其中SCAE+AVAE组效果更优，涂抹SCAE+AVAE使白细胞介素-1 （Interleukin-1，IL-1）、白细胞介素-6 （Interleukin-6，IL-6）和肿瘤坏死因子α （Tumour necrosis factor-α，TNF-α）的活性分别降低了38.93%，43.31%，46.34%。雪菊和芦荟的协同作用在护肤美容等领域具有一定应用前景。

关键词: 雪菊, 芦荟, UVB照射, 皮肤光损伤, 协同作用

Abstract:

Snow chrysanthemum and Aloe vera have great potential to protect the skin from photodamage. In this paper, we investigated the protective effects of ethanolic extracts of snow chrysanthemum and Aloe vera against outdoor UVB-induced skin photodamage in mice. According to the experimental requirements, the mice were divided into a normal control group （NC group）, a model control group （MC group）, a snow chrysanthemum alcoholic extract group （SCAE group）, a Aloe vera alcoholic extract group （AVAE group）, and a snow chrysanthemum and Aloe vera alcoholic extract group （SCAE+AVAE group）. In order to reveal the protective mechanism of alcoholic extract against photodamage in mouse skin, antioxidant indexes, hydroxyproline （HYP） content and serum-associated inflammatory factors in damaged skin were determined in mice. The results show that all three intervention groups significantly improve UVB-induced skin appearance in mice compared to the MC group. By hematoxylin-eosin staining （HE）, it indicates that the SCAE+AVAE and SCAE are more effective than the AVAE, and the mice have healthier and more elastic skin. The three intervention groups also reduce UVB-induced oxidative stress damage in mice skin, and the malondialdehyde （MDA） content of the SCAE+AVAE group is only （28.18±4.25） nmol/mgprot, which is significantly lower than that of the other groups. The activities of three antioxidant enzymes: catalase （CAT）, superoxide dismutase （SOD）, and glutathione peroxidase （GSH-Px） are also significantly higher in the SCAE+AVAE group. The SCAE+AVAE group is more effective than the alcoholic extract alone group, and the HYP content reaches （4.12±0.18） μg/mg. All three intervention groups can down-regulate the content of inflammatory factors and inhibit the inflammatory response, among which the SCAE+AVAE group is more effective. And the application of SCAE+AVAE reduces the activities of interleukin-1 （IL-1）, interleukin-6 （IL-6） and tumour necrosis factor-α （TNF-α） by 38.93%, 43.31% and 46.34%, respectively. Notably, the effect of the combined extracts is much greater than that of the extracts singly at the same dose. This study reveals the synergistic protective effect of SCAE+AVAE in the treatment of UV-induced photodamage, which is promising for skin care and cosmetic applications.

Key words: snow chrysanthemum, Aloe vera, UVB irradiation, skin photodamage, synergistic effect

中图分类号:

TQ658

李阳, 史友航, 吕青遥, 焦鸿雁, 焦士蓉. 雪菊与芦荟醇提物对UVB导致的小鼠皮肤光损伤的保护作用[J]. 日用化学工业（中英文）, 2024, 54(11): 1347-1354.

Yang Li, Youhang Shi, Qingyao Lv, Hongyan Jiao, Shirong Jiao. Protective effect of snow chrysanthemum and Aloe vera alcoholic extract on photodamage of skin in mice caused by UVB[J]. China Surfactant Detergent & Cosmetics, 2024, 54(11): 1347-1354.

图/表 7

表 1

图 1

图 2

图 3

图 4

图 5

表 2

参考文献 26

[1]	Yang Ya, Xu Lei, Liu Rongchang, et al. Effects of lavender total flavonoids on oxidative stress in skin photodamaged mice[J]. China Food Additives, 2023, 34 (1) : 172-179.
[2]	Chen Xinxin, Luo Anling, Li Jialuo, et al. Study on the anti-UVB-induced photoaging and damage of human dermal fibroblasts by polysacchride extract from Hyriopsis cumingii[J]. China Surfactant Detergent & Cosmetics, 2019, 49 (10) : 656-660.
[3]	Zhi Qi. The anthocyanin extracted from purple-fleshed sweet potato exhibited anti-photoaging effects on UVB-irradiated BALB/c-nu mouse skin[D]. Chongqing: Southwest University, 2019.
[4]	Bosch R, Philips N, Suarez-perez J A, et al. Mechanisms of photoaging and cutaneous photocarcinogenesis, and photoprotective strategies with phytochemicals[J]. Antioxidants (Basel, Switzerland), 2015, 4 (2) : 248-268.
[5]	Baliga M S, Katiyar S K. Chemoprevention of photocarcinogenesis by selected dietary botanicals[J]. Photochemical & Photobiological Sciences, 2006, 5 (2) : 243-253.
[6]	Yu Q, Chen W, Zhong J, et al. Structural elucidation of three novel oligosaccharides from Kunlun chrysanthemum flower tea and their bioactivities[J]. Food and Chemical Toxicology, 2021, 149: 112032. doi: 10.1016/j.fct.2021.112032 pmid: 33529680
[7]	Zeng Xiaoman, He Xiaoyun. Research progress on chemical composition and efficacy of Coreopsis tinctoria[J]. Science and Technology of Food Industry, 2019, 40 (13) : 335-339.
[8]	Yang Jinmei, Wang Deping. Study on the component analysis and antioxidant activity in vitro and in vivo of Kunlun chrysanthemum oleoresin[J]. Science and Technology of Food Industry, 2020, 41 (9) : 285-290.
[9]	Chen Mengting, Luo Bingjun, Yang Fangfang. Protective effect of 200∶1 Aloe vera (L.) Burm. f. Gel freeze-dried powder on gastric mucosa in rats[J]. Modern Agricultural Science and Technology, 2023 (18) : 189-192, 200.
[10]	Rodriguez R E, Darias M J, Diaz R C. Aloe vera as a functional ingredient in foods[J]. Critical Reviews in Food Science and Nutrition, 2010, 50 (4) : 305-326.
[11]	Langmead L, Makins R J, Rampton D S. Anti-inflammatory effects of Aloe vera gel in human colorectal mucosa in vitro[J]. Alimentary Pharmacology & Therapeutics, 2004, 19 (5) : 521-527.
[12]	Misawa E, Tanaka M, Saito M, et al. Protective effects of Aloe sterols against UVB-induced photoaging in hairless mice[J]. Photodermatology, Photoimmunology & Photomedicine, 2017, 33 (2) : 101-111.
[13]	Cho B O, Che D N, Shin J Y, et al. Photoprotective properties of combined extracts from Diospyros lotus leaves and Curcuma longa rhizomes against chronic UVB-induced photodamage[J]. Journal of Food Biochemistry, 2018, 42 (6) : e12672.
[14]	Ulrich-Merzenich G, Panek D, Zeitler H, et al. Drug development from natural products: exploiting synergistic effects[J]. Indian Journal of Experimental Biology, 2010, 48: 208-219. pmid: 21046973
[15]	Wu Yuxuan. Study on anti-skin photoaging effects of oral intake the complex of collagen peptides from Tilapia and natural antioxidants[D]. WuXi: Jiangnan University, 2022.
[16]	Yang Ya, Zhou Maojie, Liu Rongchang, et al. Protective mechanism of total flavonoids of Lavender against skin photodamage in mice[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28 (2) : 112-120.
[17]	Wang Shimeng, Li Tian, Yang Tianye, et al. Progress of skin photo aging models of ultraviolet irradiated experimental animals[J]. Chinese Journal of Aesthetic Medicine, 2018, 27 (7) : 146-150.
[18]	Liu Yin, Wu Fengzhi, Chen Long, et al. Determination of water-soluble protein in walnut by Bradford method[J]. China Brewing, 2013, 32 (12) : 131-133. doi: 10.3969/j.issn.0254-5071.2013.12.032
[19]	Kawada S, Ohtani M, Ishii N. Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation[J]. Am J Physiol Regul Integr Comp Physiol, 2010, 299 (2) : R694.
[20]	Kim O K, Ho J N, Nam D E, et al. Anti-wrinkle activity of a Curdrania tricuspidata extract on Ultraviolet-induced photoaging[J]. Journal of the Korean Society of Food Science & Nutrition, 2013, 42 (4) : 608-614.
[21]	Bai Y, Gu Y W, Liu S Z, et al. Flavonoids metabolism and physiological response to ultraviolet treatments in Tetrastigma hemsleyanum Diels et Gilg[J]. Frontiers in Plant Science, 2022, 13: 926197.
[22]	Martin L J. The mitochondrial permeability transition pore: a molecular target for amyotrophic lateral sclerosis therapy[J]. Biochim Biophys Acta, 2010, 1802 (1) : 186-197. doi: 10.1016/j.bbadis.2009.07.009 pmid: 19651206
[23]	Cho B O, Che D N, Shin J Y, et al. Ameliorative effects of fruit stem extract from Muscat Bailey A against chronic UV-induced skin damage in BALB/c mice[J]. Biomedicine & Pharmacotherapy, 2018, 97: 1680-1688.
[24]	Lin Dan, Jin Zhentao, Song Guoyong, et al. Research of the determination methods of hydroxyproline in collagen peptide[J]. Food Science and Technology, 2019, 44 (11) : 334-338.
[25]	Im A R, Kim H S, Hyun J W, et al. Potential for tyndalized Lactobacillus acidophilus as an effective component in moisturizing skin and anti-wrinkle products[J]. Experimental and Therapeutic Medicine, 2016, 12 (2) : 759-764. pmid: 27446272
[26]	Wang Xuehong, Yin Xingxing, Lu Jie, et al. Inhibition effects of raspberry pulp oil on photoaging of HaCaT cells induced by UVB[J]. Southwest China Journal of Agricultural Sciences, 2023, 36 (2) : 285-293.

	体重/g
	D0	D3	D5	D7	D9	D11	D14
NC组	27.61±1.35	29.48±0.36	32.27±0.37	33.82±0.57	34.43±0.28	35.78±0.41	36.24±0.51
MC组	27.79±1.65	28.29±0.34^##	29.66±0.75^##	30.75±0.54^##	32.06±0.69^##	32.99±0.64^##	33.80±0.47^##
SCAE组	27.51±1.57	29.79±0.85^**	31.61±0.35^**	32.91±0.51^**	33.72±0.59^**	34.75±0.82^**	35.68±0.33^**
AVAE组	27.72±1.11	29.24±1.32^**	30.87±1.60^**	32.32±1.13^**	33.21±0.47^**	34.27±1.45^**	35.15±0.91^**
SCAE+AVAE组	27.80±1.70	29.99±0.58^**	32.84±0.42^**	34.26±0.65^**	35.38±0.56^**	36.32±0.57^**	36.76±0.52^**

	IL-1含量/（pg/mL）	IL-6含量/（pg/mL）	TNF-α含量/（pg/mL）
NC组	133.71±17.20^a	44.37±10.13^a	178.69±19.35^a
MC组	225.71±21.38^c	92.65±9.72^c	396.13±45.33^e
SCAE组	150.25±12.33^a	70.07±12.47^b	251.68±13.92^c
AVAE组	170.45±18.79^b	65.06±5.60^b	284.18±19.57^d
SCAE+AVAE组	137.83±18.94^a	52.52±8.11^a	212.57±12.86^b