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    2024年, 第54卷, 第11期 刊出日期:2024-11-22 上一期    下一期
    基础研究
    二氧化碳在水中的相转化及其优异的抗炎生理活性
    Hydration transformation behaviors of CO2 and excellent anti-inflammatory activity on RAW 264.7 cell
    张婉萍, 顾宜婷, 李旭艳, 孙志勇, 魏春红, 张冬梅
    2024 (11):  1289-1297.  doi: 10.3969/j.issn.2097-2806.2024.11.001
    摘要 ( 1919 )   HTML ( 17 )   PDF(1353KB) ( 73 )  

    含有碳酸(H2CO3)的护肤品在全球范围内受到广泛关注。然而,二氧化碳(CO2)转化为H2CO3的过程并不稳定,H2CO3护肤的作用机理也尚未得到明确证实。本文对CO2在不同温度、pH值和压力条件下的水合-溶解行为进行了研究。此外,基于CO2水合转化现象,研究了CO2水合物对巨噬细胞(RAW 264.7)的炎症效应。结果表明,温度升高会减弱CO2的水合作用,而pH值和压力的升高都会促进CO2水相转化。当pH值小于6时,CO2与水反应生成H2CO3。当pH值在6~7之间时,提示溶液是H2CO3和HCO3-的混合物。当pH值在7~9之间时,它们主要生成HCO3-。当pH值大于9时,CO2的溶解度主要转化为CO32-。此外,CO2还能通过抑制p38蛋白的磷酸化来抑制RAW264.7细胞分泌炎症因子。CO2水合物抑制了促炎因子IL-6、TNF-α的表达,并上调了抗炎因子IL-10的表达。此外,CO2水合的抗炎分子机制是通过抑制p38的磷酸化来抑制MAPK信号通路。研究了CO2的水合溶解行为,这项研究揭示了CO2水合物的抗炎生物效应,为CO2护肤品提供了理论依据和应用支持。

    Skin care products with carbonic acid (H2CO3) have gained extensive attention worldwide. However, the conversion of CO2 to H2CO3 is not stable, and the mechanism of the effect of H2CO3 on skin care has not been clearly proved. The hydration-dissolution behaviors of CO2 were investigated under different temperature, pH, and pressure conditions. Moreover, based on the phenomenon of CO2 hydration transformation, the inflammatory effect of CO2 hydrate on macrophages (RAW 264.7) was investigated. The result shows that the increase in temperature weakened the hydration of CO2, and the increase in pH and pressure both promoted the water-phase transformation of CO2. When pH<6, CO2 reacts with water to generate H2CO3. When pH was between 6-7, the prompt solution was a mixture of H2CO3 and HCO3-. When the pH was between 7-9, they mainly generated HCO3-. And when pH>9, CO2 solubility mainly converts to CO32-. Besides, CO2 can inhibit the secretion of inflammatory factors by RAW 264.7 cells by inhibiting the phosphorylation of the p38 protein. CO2 hydrate inhibited the expression of pro-inflammatory factors IL-6, TNF-α, and up-regulated the expression of anti-inflammatory factor IL-10. Furthermore, the anti-inflammatory molecular mechanism of CO2 hydration inhibited the MAPK signaling pathway by inhibiting the phosphorylation of p38. The hydration-dissolution behavior of CO2 was investigated. This work revealed the anti-inflammatory bioeffect of CO2 hydrate, providing a theoretical basis and application support for CO2 skin care products.

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    科技讲座
    天然生物质材料的制备、性质与应用(Ⅺ)——高支化的高浓低黏植物多糖:阿拉伯胶
    Preparation, properties and applications of natural biomass materials (Ⅺ)Highly branched, high concentration-low viscosity plant polysaccharides: arabic gum
    赵以墨, 王嘉麟, 吴放, 熊海平, 薛鹏程, 范金石
    2024 (11):  1298-1306.  doi: 10.3969/j.issn.2097-2806.2024.11.002
    摘要 ( 1338 )   HTML ( 7 )   PDF(2066KB) ( 43 )  

    阿拉伯胶是一种来源于合金欢树(Acacia sengal (L.) Willdenow)及其它亲近树种的具有高度支化结构和复杂组成的天然植物胶。因阿拉伯胶具有良好的水溶性、增稠性、乳化性、稳定性等性质以及安全无毒、生物相容性良好、可生物降解的天然属性,且利用其分子结构中的羟基、糖苷键等活性官能团进行物理、化学、酶处理及共混/复合后能显著改善其理化性质、增强其功效性能,使得阿拉伯胶及其改性产物和复合物在日用化工、食品、医药、纺织印染、油墨、功能材料等多个行业领域得到广泛应用。本文浅述了阿拉伯胶的名称、来源、结构和典型理化性质与功能特性,并较为系统地介绍了阿拉伯胶的提取方法和再加工工艺,同时介绍了阿拉伯胶及其改性产物和复合物的应用情况,并提及了阿拉伯胶及其改性产物和复合物当前所面临的主要问题及今后的研发方向与发展前景。

    Arabic gum is one kind of natural plant gum derived from Acacia sengal (L.) Willdenow and other related tree species, characterized by its highly branched structure and complex composition. Arabic gum possesses excellent water solubility, thickening property, emulsifying ability, and stability properties, as well as safe, non-toxic nature, good biocompatibility, and biodegradability. Moreover, both its physicochemical properties and functional performance can be further improved by conducting physical, chemical, enzymatic treatments and blending/composite processing with the active functional groups, such as hydroxyl groups and glycosidic bonds, in the arabic gum molecular structure. As a result, arabic gum, its modified products and composites have been widely used in various industries, including daily-use chemical industry, food processing industry, pharmaceutical industry, textile industry, printing and dyeing industry, ink industry, and functional materials manufacturing industry. In this paper, the name, source, chemical structure, main physicochemical properties and functional characteristics of arabic gum are briefly introduced. Its extraction methods and reprocessing techniques are also systematically presented. Furthermore, the application status of arabic gum and its modified products, its composites, as well as the main issues currently faced by them are together summarized. Meanwhile, some future research and development directions and prospects of arabic gum are properly pointed out.

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    开发与应用
    海上高含水油田用无机复合调驱剂实验研究
    Experimental research of inorganic composite deep profile control and oil displacement agent for offshore high water cut oil fields
    于萌, 徐国瑞, 李翔, 郑玉飞, 冯轩, 杨劲舟, 刘丰钢
    2024 (11):  1307-1312.  doi: 10.3969/j.issn.2097-2806.2024.11.003
    摘要 ( 1906 )   HTML ( 8 )   PDF(1429KB) ( 62 )  

    针对海上碳酸氢钠水型的高含水油田,以硅酸钠和氯化钙为主剂、聚羧酸分散剂和乳液聚合物为助剂制备了环保型无机复合调驱剂,并研究了聚羧酸分散剂类型和乳液聚合物质量浓度对调驱剂性能的影响规律。实验结果表明,当聚羧酸分散剂的酸醚比为3.7、分子量为23 800时,聚羧酸分散剂和无机颗粒表面的静电斥力和空间位阻作用相互促进,可达到有效分散效果。配合使用低质量浓度乳液聚合物(500 mg/L),形成的无机复合调驱体系可进一步增强体系的黏度和强度,初始黏度从1.1 mPa·s提升至5.4 mPa·s。封堵实验表明,无机复合调驱剂能够大幅度增加流动阻力,封堵率可达82.3%。该无机复合调驱剂可实现在线注入,具有快速分散、深部调驱和价格低廉等特点,在海上及其他作业空间受限的高含水油田中具有良好应用前景。

    An environmentally friendly inorganic composite deep profile control and oil displacement agent was prepared for those offshore high water cut oil fields with formation water containing sodium bicarbonate. Sodium silicate and calcium chloride were used as the main agents, while polycarboxylic acid dispersant and emulsion polymer were used as assistants. The effects of the type of polycarboxylic acid dispersant and the mass concentration of emulsion polymer on the performance of the deep profile control and oil displacement agent were studied. The results showed that when the acid-ether ratio of the polycarboxylic acid dispersant was 3.7 and its molecular weight was 23 800, the electrostatic repulsion and steric hindrance from polycarboxylic acid dispersant and inorganic particles achieved an effective dispersing effect. Combined with low concentration of emulsion polymer (500 mg/L), the formulated inorganic composite deep profile control and oil displacement system could enhance the viscosity and strength of the system, and the initial viscosity was increased from 1.1 mPa·s to 5.4 mPa·s. The results showed that this inorganic composite deep profile control and oil displacement agent could increase the flow resistance, with a plugging rate of 82.3%. It could achieve online injection and had the characteristics of rapid dispersing, deep profile control and oil displacement, and low cost. This inorganic composite deep profile control and oil displacement agent had application value in high water cut oil fields.

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    重组人钙网蛋白促雄激素性脱发小鼠毛发再生的作用研究
    Recombinant human calreticulin promotes hair regeneration in androgenetic alopecia mice
    贺改英, 吴首标, 刘树涛, 魏东东, 崔俊生, 王毅
    2024 (11):  1313-1319.  doi: 10.3969/j.issn.2097-2806.2024.11.004
    摘要 ( 1331 )   HTML ( 7 )   PDF(2705KB) ( 38 )  

    研究重组人钙网蛋白(recombinant human calreticulin,rh-CRT)促雄激素性脱发(androgenetic alopecia,AGA)小鼠毛发再生的作用。建立AGA小鼠模型,局部应用rh-CRT,研究rh-CRT对毛发再生的影响。通过观察不同时间毛发整体外观和局部外观评估毛发的再生,采用颜色测试仪检测皮肤的黑色素指数,利用皮肤镜观察不同类型毛发直径的变化,扫描电镜检测毛小皮的改变,并进一步通过HE染色研究皮肤和真皮脂肪厚度以及毛囊的数量。结果发现,rh-CRT促进AGA小鼠毛发的再生,增加黑色素指数,并显著提高AGA小鼠粗毛和细毛的直径,修复睾酮组(testosterone,TES)诱导的毛小皮的损伤。从皮肤冠状面观察发现rh-CRT显著促进AGA小鼠毛囊数量的表达,并增加皮肤和真皮脂肪的厚度。这些结果表明rh-CRT可以促进AGA小鼠毛发的再生,在防脱产品中具有重要的应用价值。

    Androgenetic alopecia (AGA) is the most common type of hair loss and is usually caused by multiple factors. AGA is characterized by progressive miniaturization of hair follicles, shortened anagen phase, prolonged telogen phase, and the decrease of hair density. We studied the role of recombinant human calreticulin (rh-CRT) in promoting hair regeneration in androgenetic alopecia (AGA) mice. Hair regeneration was assessed by observing the overall and local appearance of the hair at different time points, the melanin index of the skin was observed by color tester, the change of hair diameter was detected by dermatoscopy, the change of the hair cuticle was studied by scanning electron microscopy, and the thickness of the skin and the dermal white adipose, and the number of hair follicles were further observed by HE staining. The results reveal that rh-CRT promotes hair regeneration, increases melanin index, significantly improves the diameters of coarse and fine hairs in AGA mice, and repairs the damaged hair cuticle induced by testosterone(TES). In addition, it is demonstrated that rh-CRT significantly promotes the number of hair follicles and the thickness of the skin and dermal white adipose in AGA mice. The results suggest that rh-CRT promotes the regeneration of hair in AGA mice, which has important application value in hair regeneration products.

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    基于蜡酯型乳化剂的液晶结构乳状液的制备研究及应用性能表征
    Preparation and evaluation of the liquid crystal emulsion based on wax ester emulsifier
    沈玲华, 庄洁, 张瑾, 张倩洁, 张婉萍
    2024 (11):  1320-1329.  doi: 10.3969/j.issn.2097-2806.2024.11.005
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    研究了制备工艺和配方组成(油脂、多元醇、流变调节剂和离子性成分)对蜡酯型乳化剂液晶结构形成的影响,并对其液晶结构乳状液进行了相关的应用性能测试。实验结果表明,一罐法制备乳液更有利于该乳化剂配方体系液晶结构的形成。该乳化剂与植物油脂相溶性较好,乳化能力较佳,对于植物来源酯类油脂,遵循油水界面张力越小,乳化效果越佳,越有利于液晶结构形成的规律;甘油的添加能使体系保持最初优异的液晶结构及其稳定性,但1,3-丁二醇会由于其氢键与水形成的水合基团扰乱界面膜分子排列,从而使体系的液晶结构完整性及稳定性有所降低;离子性成分的添加可通过增加乳化剂紧密排列来明显增强体系的液晶结构;天然流变调节剂的加入,如AX和Tara,可以增强体系液晶结构的高温稳定性,而卡波类可增强其低温稳定性。同时,通过对液晶结构乳状液应用性能的研究发现,使用蜡酯型乳化剂制备得到的液晶结构乳状液保湿性能明显优于普通结构乳状液,且具有一定的缓释作用。

    The influences of preparation technology and formula composition (oils, polyols, rheological regulators and ionic components) on the formation of liquid crystal structure in the emulsion stabilized with wax ester emulsifier were systematically studied, and the application performance of the liquid crystal emulsion was also tested. The experimental results showed that the one-pot method was more conducive to the formation of liquid crystal structure of the emulsion system. The emulsifier had good compatibility with vegetable oil and good emulsifying ability. For plant-origin oils, the smaller the interfacial tension between oil and water, the better the emulsifying effect and the more conducive to the formation of liquid crystal structure. The addition of glycerol could maintain the original excellent liquid crystal structure and stability of the system, but 1,3-butanediol would disrupt the molecular arrangement in the interfacial layer due to the hydrated groups formed by hydrogen bonding with water, thus reducing the integrity and stability of the liquid crystal structure of the system; the addition of ionic components could significantly enhance the liquid crystal structure of the system by increasing the compactness of the arrangement of emulsifiers; the addition of natural rheological regulators, such as AX and Tara, could enhance the high-temperature stability of the liquid crystal structure of the system, while Carbopol® type could enhance its low-temperature stability. Meanwhile, by study of the application performance of liquid crystal emulsion, it was found that the liquid crystal emulsion prepared with wax ester emulsifier had better moisturizing performance than the ordinary structured emulsion, and had certain slow-release effect.

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    Au NPs/Cu-TCPP材料的构筑及其催化薄荷醇氧化的研究
    Preparation of Au NPs/Cu-TCPP material and its performance in catalytic oxidation of menthol
    李世叶, 张剑, 王杰, 杨超, 张晋, 成世兴
    2024 (11):  1330-1338.  doi: 10.3969/j.issn.2097-2806.2024.11.006
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    二维纳米片具有大的比表面积和高孔隙率的特征,这使得其可以承载更多的活性中心,从而提高该材料的催化性能。如Cu-TCPP纳米片,拥有大的比表面积,可用于负载Pd NPs、Pt NPs以及Au NPs,以获得X NPs/Cu-TCPP纳米片(X=Pd NPs、Pt NPs和Au NPs)。本研究首先通过水热法,以三水合硝酸铜(Cu(NO32·3H2O)为金属盐,4-羧基卟啉(TCPP)为有机配体,N,N-二甲基甲酰胺(DMF)和CH3CH2OH(体积比3∶1)的混合溶液作为溶剂,合成Cu-TCPP纳米片。随后,采用还原法将Au NPs负载到Cu-TCPP纳米片上,形成Au NPs/Cu-TCPP纳米片,用于催化薄荷醇进行选择性氧化反应。在反应中,以叔丁基过氧化氢(TBHP)为氧化剂,乙腈(MeCN)为溶剂,氨基酸咪唑离子液体([C4mim]Cys)为助催化剂,Au NPs/Cu-TCPP催化薄荷醇进行选择性氧化,生成薄荷酮。在50 ℃下反应18 h,薄荷醇的转化率和薄荷酮的选择性和产率都达到最高,分别为70.2%,99.9%和69.5%,该方法不仅将薄荷醇转化为高选择性的薄荷酮,而且操作简便,绿色环保,符合当前绿色化工发展的要求。

    Two-dimensional nanosheets have the characteristics of large specific surface area and high porosity, which allow them to support more active centers, thus improving the catalytic performance of the material. For example, Cu-TCPP nanosheets have large specific surface area and can be loaded with Pd nanoparticles (NPs), Pt NPs and Au NPs to obtain X NPs/Cu-TCPP nanosheets (X=Pd NPs, Pt NPs and Au NPs). In this work, Cu-TCPP nanosheets were synthesized by hydrothermal method, in which Cu(NO32·3H2O was used as the metal salt, TCPP (4-carboxyporphyrin) was used as the organic ligand, and a mixed solution of DMF and CH3CH2OH (VDMF/VCH3CH2OH=3∶1) was used as the solvent. Then Au NPs were loaded onto Cu-TCPP nanosheets by reduction method to form Au NPs/Cu-TCPP nanosheets, which were employed to catalyze the selective oxidation of menthol. In the reaction, with tert-butyl hydroperoxide (TBHP) as oxidant, acetonitrile (MeCN) as solvent, and the amino acid imidazolium ionic liquid [C4mim]Cys as cocatalyst, Au NPs/Cu-TCPP catalyzed the selective oxidation of menthol to obtain menthone. After reacting at 50 ℃ for 18 h, the conversion of menthol was up to 70.2%, the selectivity and yield of menthone could reach 99.9% and 69.5%. This method not only highly selectively convert menthol to menthone, but also was easy to operate and environmentally friendly, which could meet the current development requirements of the green chemical industry.

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    亚微米二氧化钛基功效性紫外屏蔽复合材料的制备及性能研究
    Preparation and properties of submicron titanium dioxide based efficient UV shielding composites
    吴鑫, 左士祥, 余瑶瑶, 孙晓敏, 蔡悦, 姚超
    2024 (11):  1339-1346.  doi: 10.3969/j.issn.2097-2806.2024.11.007
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    首先制备了一种亚微米花状二氧化钛,依次对其进行无机和有机表面处理后制得TiO2/Al(OH) 3/SS复合材料(TAS),最后在TAS表面包覆一种天然防晒剂姜黄素(Cur),得到最终产物TASC复合材料。通过XRD、UV-Vis、TEM、FT-IR、水接触角等技术手段对所得样品进行表征,结果表明:Al(OH) 3、SS、Cur均以非晶态和物理吸附的形式包覆于TiO2表面,三者的适宜包覆量分别为8%,8%和6%;防晒指数分析表明:TASC的紫外屏蔽能力远强于市售亚微米TiO2(CR-50),接近市售纳米TiO2 (626B)。此外,与TAS相比,TASC复合材料还具有较强的抗氧化性。

    In this study, a sub-micron flower-like titanium dioxide was first prepared, followed by inorganic and organic surface treatment to prepare TiO2 /Al(OH) 3 /SS composite (TAS). Finally, the surface of TAS was coated with curcumin (Cur), a natural sunscreen, to obtain the final product-TASC composite. The samples were characterized by XRD, UV-Vis, TEM, FT-IR and water contact angle. The results show that Al(OH) 3, SS and Cur are all coated on the TiO2 surface in the form of amorphous state and physical adsorption, and the suitable coating amounts of the three are 8%, 8% and 6%, respectively. The UV shielding ability of TASC is much stronger than that of the commercial submicron TiO2 (CR-50) and close to the level of the commercial nano TiO2 (626B). In addition, compared with TAS, TASC composite also has strong oxidation resistance.

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    雪菊与芦荟醇提物对UVB导致的小鼠皮肤光损伤的保护作用
    Protective effect of snow chrysanthemum and Aloe vera alcoholic extract on photodamage of skin in mice caused by UVB
    李阳, 史友航, 吕青遥, 焦鸿雁, 焦士蓉
    2024 (11):  1347-1354.  doi: 10.3969/j.issn.2097-2806.2024.11.008
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    雪菊和芦荟在保护皮肤光损伤方面有极大的潜力。文章研究了雪菊和芦荟的乙醇提取物对户外紫外线(Ultraviolet radiation B,UVB)诱导的小鼠皮肤光损伤的保护作用。实验将小鼠分为正常对照组(NC组)、模型对照组(MC组)、雪菊醇提物组(SCAE组)、芦荟醇提物组(AVAE组)、雪菊芦荟醇提物组(SCAE+AVAE组)。通过对受损皮肤中抗氧化指标、羟脯氨酸(Hydroxyproline,HYP)含量和小鼠血清相关炎症因子的测定,初步揭示预防小鼠皮肤光损伤的作用机制。结果表明,与MC组相比,三个干预组对UVB诱导的小鼠皮肤外观均有明显改善,通过苏木精-伊红染色法(Hematoxylin-eosinstaining,HE)得出,SCAE+AVAE组和SCAE组比AVAE组效果更好,小鼠皮肤更加健康有弹性。三个干预组也可降低UVB诱导小鼠皮肤的氧化应激损伤,SCAE+AVAE组丙二醛(Malondialdehyde,MDA)含量仅为(28.18±4.25) nmol/mgprot,显著低于其他组;SCAE+AVAE组三种抗氧化酶:过氧化氢酶(Catalase,CAT)、超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)活性也显著升高。SCAE+AVAE组较单独醇提物组效果更佳,HYP含量达到了(4.12±0.18) μg/mg。三个干预组均可下调炎症因子含量,抑制炎症反应,其中SCAE+AVAE组效果更优,涂抹SCAE+AVAE使白细胞介素-1 (Interleukin-1,IL-1)、白细胞介素-6 (Interleukin-6,IL-6)和肿瘤坏死因子α (Tumour necrosis factor-α,TNF-α)的活性分别降低了38.93%,43.31%,46.34%。雪菊和芦荟的协同作用在护肤美容等领域具有一定应用前景。

    Snow chrysanthemum and Aloe vera have great potential to protect the skin from photodamage. In this paper, we investigated the protective effects of ethanolic extracts of snow chrysanthemum and Aloe vera against outdoor UVB-induced skin photodamage in mice. According to the experimental requirements, the mice were divided into a normal control group (NC group), a model control group (MC group), a snow chrysanthemum alcoholic extract group (SCAE group), a Aloe vera alcoholic extract group (AVAE group), and a snow chrysanthemum and Aloe vera alcoholic extract group (SCAE+AVAE group). In order to reveal the protective mechanism of alcoholic extract against photodamage in mouse skin, antioxidant indexes, hydroxyproline (HYP) content and serum-associated inflammatory factors in damaged skin were determined in mice. The results show that all three intervention groups significantly improve UVB-induced skin appearance in mice compared to the MC group. By hematoxylin-eosin staining (HE), it indicates that the SCAE+AVAE and SCAE are more effective than the AVAE, and the mice have healthier and more elastic skin. The three intervention groups also reduce UVB-induced oxidative stress damage in mice skin, and the malondialdehyde (MDA) content of the SCAE+AVAE group is only (28.18±4.25) nmol/mgprot, which is significantly lower than that of the other groups. The activities of three antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) are also significantly higher in the SCAE+AVAE group. The SCAE+AVAE group is more effective than the alcoholic extract alone group, and the HYP content reaches (4.12±0.18) μg/mg. All three intervention groups can down-regulate the content of inflammatory factors and inhibit the inflammatory response, among which the SCAE+AVAE group is more effective. And the application of SCAE+AVAE reduces the activities of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) by 38.93%, 43.31% and 46.34%, respectively. Notably, the effect of the combined extracts is much greater than that of the extracts singly at the same dose. This study reveals the synergistic protective effect of SCAE+AVAE in the treatment of UV-induced photodamage, which is promising for skin care and cosmetic applications.

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    甘草酸二钾通过调节巨噬细胞极化促进皮肤修复
    Dipotassium glycyrrhizinate promotes skin repair by regulating macrophage polarization
    王学文, 刘慧, 王倩, 杨素珍, 刘菲
    2024 (11):  1355-1361.  doi: 10.3969/j.issn.2097-2806.2024.11.009
    摘要 ( 99 )   HTML ( 8 )   PDF(1705KB) ( 47 )  

    为研究甘草酸二钾促进皮肤修复的作用机理及其与炎症反应的关系,用不同质量分数的甘草酸二钾处理LPS诱导的小鼠单核巨噬细胞(RAW264.7)炎症模型,通过流式细胞术、免疫荧光和ELISA的方法研究不同关键指标的变化。通过用甘草酸二钾和甘草酸二钾处理的RAW264.7细胞的培养液作为刺激物,处理人永生化角质形成细胞(HaCaT)细胞划痕模型,研究甘草酸二钾对皮肤修复作用的机制。结果显示,经不同质量分数的甘草酸二钾处理的RAW264.7细胞炎症模型,细胞膜上TLR2的表达降低,CD163的表达增加;RAW264.7细胞内的ROS水平降低;上清液中的NO,TNF-α和IL-6的含量显著降低,IL-4和IL-10的含量显著升高;用0.125%(w/%)甘草酸二钾处理的RAW264.7细胞的培养液作用于HaCaT细胞24 h,观察到HaCaT细胞迁移能力显著增强,然而甘草酸二钾直接作用于HaCaT细胞不能显著促进细胞迁移能力。综上,甘草酸二钾能够通过改善LPS诱导的RAW264.7细胞M1极化,调节炎症因子的分泌,达到促进HaCaT细胞迁移、促进皮肤修复的作用。

    The research aimed to study the mechanism of dipotassium glycyrrhizinate in promoting skin repair and its relationship with inflammatory reaction. The inflammatory model of mouse monocyte macrophage (RAW264.7) induced by LPS was treated with different mass fractions of dipotassium glycyrrhizinate, and the expressions of TLR2 and CD163 on the surface of RAW264.7 cells were detected by flow cytometry. The results show that dipotassium glycyrrhizinate inhibits the expression of TLR2 on the cell membrane and promotes the expression of CD163, indicating that dipotassium glycyrrhizinate inhibits the M1 polarization of macrophages and promotes the M2 polarization. It is confirmed by immunofluorescence that dipotassium glycyrrhizinate can prevent M1 polarization by inhibiting the production of ROS in macrophages. By detecting the NO content and the secretion of TNF-α, IL-4, IL-6 and IL-10, the inflammatory factors and mediators related to M1/M2 polarization were studied. Among them, the contents of NO, TNF-α and IL-6 decrease, while the contents of IL-4 and IL-10 increase, which prove that dipotassium glycyrrhizinate regulates LPS-induced M1 polarization of macrophages and promotes the secretion of M2 polarization marker protein. In order to study the mechanism of promoting skin repair by dipotassium glycyrrhizinate, the scratch and proliferation experiments of HaCaT human keratinocytes (HaCaT) were carried out with different mass fractions of dipotassium glycyrrhizinate and the culture medium of RAW264.7 cells treated with dipotassium glycyrrhizinate. The results confirm that dipotassium glycyrrhizinate can promote the proliferation of HaCaT cells, but can not directly promote the migration of HaCaT cells. However, it can promote the migration and proliferation of HaCaT cells by regulating the polarization of RAW264.7 cells. To sum up, dipotassium glycyrrhizinate can improve the M1 polarization of RAW264.7 cells induced by LPS, regulate the secretion of inflammatory factors, and promote the migration of HaCaT cells and skin repair.

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    织物柔顺剂洗衣伴侣的性能测试与研究
    Research and analysis on the performance of a laundry companion as fabric softener
    冯逸鑫, 叶嘉烘, 翁佳丽, 王佳丽, 李燕, 张磊
    2024 (11):  1362-1367.  doi: 10.3969/j.issn.2097-2806.2024.11.010
    摘要 ( 54 )   HTML ( 6 )   PDF(1832KB) ( 26 )  

    测试织物柔顺剂洗衣伴侣的织物护理性能及其使用方式。对洗衣伴侣洗涤后的织物进行去污力、抗静电、再润湿性、感官触摸柔软度和稳定性测试,综合评价洗衣伴侣在织物护理方面的性能优势以及同步洗衣液添加法对洗涤去污力的影响。经过检测,织物柔顺剂洗衣伴侣与洗衣液同步进行添加洗涤后,3块污布去污比值均达到1.0,符合洗衣液产品行业标准;经洗衣伴侣处理后的织物,表征抗静电性能的表面比电阻对数值(∆lg ρs)降低4.0,再润湿性(以毛细效应时间计)达到20 s,感官触摸柔软度评价总得分86分,平均4.3分。与市售织物柔顺剂相比,各方面性能提升明显,具有良好的织物柔顺性能。洗衣伴侣较常规织物柔顺剂具有更简便的使用方法,并且在织物柔顺方面性能优势明显。为洗衣行业产品的不断创新增添新动力,也为行业发展提供一个参考和思路。

    By testing the fabric-care performance of a laundry companion which was a new fabric softener and how to use it, the data support for marketing this type of products was provided. The detergency, antistatic property, rewetting, tactile softness and stability were tested. The advantages of the laundry companion in fabric care and the influence of synchronous detergent addition on the detergency for washing were comprehensively evaluated. The detersive ratios for the three soiled cloths all reached 1.0 if the new fabric softener and laundry liquid were simultaneously added, which could meet the industrial standard of laundry liquid products. After treatment with the laundry companion, the logarithm of surface specific resistance (∆lg ρs) which represented the antistatic property of the fabric, was decreased by 4.0, the rewettability as measured by “capillary effect time” reached 20 s, and the average score for evaluation of tactile softness given by 20 testers was 4.3 points (5 points for full marks). Compared with the fabric treated with commercial fabric softener, the properties of the one treated with laundry companion were significantly improved, indicative of good fabric-softening ability. Compared with conventional fabric softeners, this laundry companion had a simpler method of use and had obvious advantages in fabric softening. This work could add new impetus to the continuous innovation of products in laundry industry, and also provide a reference for the development of the industry.

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    异落新妇苷的美白作用及抗氧化作用研究
    Study on the whitening effect and antioxidation of isoastilbin
    刘慧, 刘芳
    2024 (11):  1368-1374.  doi: 10.3969/j.issn.2097-2806.2024.11.011
    摘要 ( 61 )   HTML ( 6 )   PDF(1520KB) ( 38 )  

    为了揭示异落新妇苷(isoastilbin,ISO)的美白作用及抗氧化作用。建立了紫外线B(UVB)诱导的皮肤光老化(SP)小鼠模型,使用3个剂量(1,2,4 mg/cm2)的异落新妇苷对小鼠进行经皮给药。治疗8周后,检测了小鼠的表皮含水量。通过HE染色和Masson三色染色观察皮肤表皮和真皮形态。按试剂盒说明书检测皮肤氧化应激指标(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA))水平。采用NaOH溶解法检测皮肤黑色素含量。采用酶联免疫吸附剂测定(ELISA)法检测皮肤酪氨酸酶(TYR)活性。通过RT-qPCT检测皮肤基质金属蛋白酶-1(MMP-1)和Collagen Ⅰ的mRNA水平。通过Western blotting检测皮肤细胞核核因子红细胞2相关因子2 (Nrf2)的蛋白表达水平。结果显示,异落新妇苷剂量依赖性地提高了UVB诱导的SP小鼠的表皮含水量,降低了表皮厚度,减少了胶原纤维变性,降低了MMP-1的mRNA相对表达量,升高了CollagenⅠ的mRNA相对表达量,升高了SOD、CAT和GSH-Px活性,降低了MDA含量,升高了细胞核Nrf2的蛋白相对表达量,降低了黑色素含量和TYR活性(P<0.05)。本研究表明,异落新妇苷可减轻UVB诱导的小鼠SP,并具有美白作用,其机制与提高皮肤抗氧化能力以及降低黑色素含量和TYR活性有关。

    The study aimed to reveal the whitening effect and antioxidant effect of isoastilbin (ISO). In this study, a mouse model of skin photoaging (SP) induced by ultraviolet B (UVB) was established. Three doses (1, 2, 4 mg/cm2)of isoastilbin were administered transcutaneously in mice. After 8 weeks of treatment, the water content of mouse epidermis was detected. The morphology of epidermis and dermis was observed by HE staining and Masson trichrome staining. The levels of skin oxidative stress indicators (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA)) were measured according to the instructions of the kit. The content of melanin in skin was detected by NaOH lysis method. The activity of tyrosinase (TYR) in the skin was detected by enzyme-linked immunosorbent assay (ELISA). The mRNA levels of matrix metalloproteinase-1(MMP-1) and Collagen I were detected by RT-qPCT. The protein expression of nuclear factor E2-related factor 2 (Nrf2) in the skin cells was detected by Western blotting. The results show that isoastilbin increases the water content of epidermis, decreases the thickness of epidermis, reduces the degeneration of collagen fibers, decreases the relative expression of mRNA of MMP-1, increases the relative expression of mRNA of Collagen I, increases the activities of SOD, CAT and GSH-Px, decreases the content of MDA, increases the relative expression of nuclear Nrf2 and decreases the content of melanin and TYR in SP mice induced by UVB in a dose-dependent manner (P<0.05). This study shows that isoastilbin can reduce UVB-induced SP in mice and has a whitening effect, which is related to the improvement of skin antioxidant capacity and the decrease of melanin content and TYR activity.

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    基于光损伤皮肤成纤维细胞模型的鱼胶原蛋白肽修复性能研究
    Study on the repair performance of fish collagen peptides based on the UVA induced fibroblast photodamage model
    蒲春宏, 王荣昌, 华中杰, 张佳婵, 陈朝
    2024 (11):  1375-1381.  doi: 10.3969/j.issn.2097-2806.2024.11.012
    摘要 ( 60 )   HTML ( 6 )   PDF(1319KB) ( 50 )  

    鱼胶原蛋白肽(Fish collagen peptides,FCPs)是一类具备多种活性功能的多肽混合物。近几年研究表明,口服鱼胶原蛋白肽能够安全高效地作用于皮肤,发挥抗氧化、抑制黑色素形成、延缓衰老和抗光老化等功效。然而,现有鱼胶原蛋白肽的体外功效研究较少。该研究利用人皮肤成纤维细胞建立UVA诱导的光损伤模型,从细胞形态和细胞存活率两个方面研究鱼胶原蛋白肽对人皮肤成纤维细胞增殖能力的影响。通过测定鱼胶原蛋白肽处理细胞后,其中透明质酸、Ⅰ型胶原、弹性蛋白含量以及总抗氧化能力的变化来研究其抗氧化功效。另外对比分析细胞形态、细胞活力和关键代谢产物等的具体变化来研究鱼胶原蛋白肽对UVA诱导的光损伤细胞的修复作用。该研究为鱼胶原蛋白肽在皮肤光损伤修复领域提供了理论依据,为新型功能食品和胶原蛋白产品的开发与应用奠定了基础。

    Fish collagen peptides (FCPs) represent a class of polypeptide mixtures endowed with diverse bioactive functions. Recent studies indicate that orally administered fish collagen peptides can effectively and safely impact the skin, exhibiting benefits such as antioxidation, inhibition of melanin formation, delayed aging, and resistance to photoaging. However, research comprehensively evaluating the efficacy of fish collagen peptides at the cellular level is relatively scarce. This study established a UVA-induced photodamaged human skin fibroblast model to investigate the impact of collagen peptides on the proliferation of human skin fibroblasts from both cellular morphology and viability perspectives. Additionally, alterations in hyaluronic acid, type Ⅰ collagen, elastin content, and total antioxidant capacity were assessed after treating cells with different samples. Through a comprehensive examination encompassing morphology, cell viability, and crucial metabolic substances, the photodamage repair effect of fish collagen peptides were comprehensively evaluated. This research provides experimental data support for oral collagen peptides in the field of skin photodamage repair, laying the groundwork for the development and application of novel functional foods and collagen products in the future.

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    专论与综述
    皮肤微生态调节型产品的评价方法概述
    Efficacy assessment for skin microecology/microbiome-modulating cosmetics
    郑玉梅, 胡熔, 吴文海, 宋丽雅, 王闻
    2024 (11):  1382-1390.  doi: 10.3969/j.issn.2097-2806.2024.11.013
    摘要 ( 955 )   HTML ( 5 )   PDF(1139KB) ( 930 )  

    综述了皮肤微生态调节型产品的评价方法,包括体外和人体测试方法,用于指导相关产品的研发与评价。化妆品可以通过直接改变皮肤微生物数量、生长代谢、群落结构、群体效应或通过调节皮肤免疫反应、改善皮肤生理功能等方式调节皮肤微生态平衡。皮肤微生物的数量、生长代谢水平、群体效应、皮肤免疫反应状态等评价指标常采用体外法。皮肤微生物的多样性、群落结构和皮肤生理状态常采用人体测试法,并结合16S rRNA扩增子测序、ITS扩增子测序、鸟枪法宏基因组测序等高通量测序技术。不同评价方法评估的维度和优势不同,体外法更具有靶向性,条件易于控制,但模型过于简单缺乏活性;相比之下,人体测试法更具有全观性,更符合真实使用场景,但很难定性个体差异、产品使用与微生物组变化之间复杂的因果关系。在评价过程中采用体外和人体测试相结合的方式,可以一定程度上互补不同评价方法的局限性,能为产品的效果提供更完整的证据链。

    The in vitro and in vivo evaluation methods of microecological skin care products were summarized. Cosmetics can regulate skin microecological balance by directly changing the number, growth metabolism, community structure, and population effect of skin microorganisms, or by regulating skin immune response and improving skin physiological function. The number, growth and metabolism level, population effect of skin microorganisms and skin immune response status are often evaluated by in vitro method. Skin microbial diversity, community structure, and skin physiological state are often tested by human methods, combined with high-throughput sequencing technologies such as 16S rRNA amplicon sequencing, ITS amplicon sequencing, and shotgun metagenomic sequencing. Different evaluation methods have different dimensions and advantages. The in vitro method is more targeted and the test conditions are easy to control, but the model is too simple and lack of activity. In contrast, clinical trials are more holistic and in line with real use scenarios, but it is difficult to qualitative analyze the complex causal relationship among individuals, product use and microbiome changes. In the process of efficacy evaluation, the combination of in vitro and in vivo can complement the limitations of different evaluation methods to a certain extent, and can provide a more complete evidence chain for the efficacy evaluation of products.

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    分析与检测
    基于PMA-LAMP技术可视化检测消毒产品消毒效果
    Visual detection of the disinfection effect of disinfectants by loop-mediated isothermal amplification combined with propidium monoazide
    梁媛媛, 夏琪琪, 杨启立, 徐新怡, 周志南, 刘鹏鹏
    2024 (11):  1391-1396.  doi: 10.3969/j.issn.2097-2806.2024.11.014
    摘要 ( 47 )   HTML ( 6 )   PDF(1540KB) ( 19 )  

    探讨建立一种氮溴化丙锭(Propidium monoazide,PMA)染料与环介导等温扩增法(Loop-mediated isothermal amplification,LAMP)联用的技术,实现可视化判断消毒产品消毒效果的目的。随机选取5种成分不同的市售消毒产品,回收消毒产品消毒处理后的菌液并进行PMA处理,并对处理后的菌液进行细菌基因组DNA提取。将大肠杆菌作为消毒效果检测的指示菌,针对大肠杆菌的fecA基因以及针对存在于所有细菌的16S rRNA基因进行LAMP扩增,并在反应体系中加入钙黄素染料。通过肉眼观察反应管颜色变化即可对消毒产品的消毒效果进行判断。将PMA-LAMP方法与PMA-qPCR方法进行比较后发现PMA-LAMP方法在操作步骤、检测时长、灵敏度方面均优于PMA-qPCR方法。在对消毒产品消毒效果的实际检测中,PMA-LAMP方法与《消毒技术规范(2002年版)》规定的标准方法的结果基本一致,两者杀灭对数值偏差在±0.5以内。该方法不依赖大型精密温控仪器,检测过程为45 min(不包括前处理步骤),实现了对消毒产品消毒效果现场快速可视化检测,具有良好的基层应用前景,将为消毒产品的性能评价提供参考、为行业市场监管提供技术支持。

    For visual detection of the disinfection effect of disinfectant products, a new method combining propidium monoazide (PMA, a dye) with loop-mediated isothermal amplification (LAMP) was established. Five commercial disinfection products with different ingredients were randomly selected in this research. Glass slides covered with bacteria were freshly prepared before use. After disinfection treatment by the disinfection products, the bacteria solutions were collected and then treated with PMA separately. Bacterial genomic DNA was extracted from the bacterial solutions. Subsequently, gDNA was used as template for LAMP and real-time PCR for amplification of fecA gene of Escherichia coli and 16S rRNA which universally existed in bacteria. Meanwhile, by adding calcein to the reaction system, the disinfection effect could be determined. The color change from fluorescent green to light orange in reaction tube could be observed by naked eyes. Excluding the step of disinfection treatment, the detection procedure could be completed within 45 min. Compared with PMA-qPCR, PMA-LAMP had the advantages of easy to operate, more sensitive, faster in detection, and no need of precise temperature-controlling instruments. For testing the disinfection effects of actual disinfection products, the results of PMA-LAMP were basically consistent with those of the standard method (refer to Technical Standard for Disinfection (2002 edition)), with a deviation within±0.5 between the two methods. The newly established PMA-LAMP method had good application prospect in field experiments. It would provide technical support for performance evaluation of disinfection products and market supervision.

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    高效液相色谱法测定牙膏中防腐剂使用情况的研究
    Study on the using status of preservatives in toothpastes based on HPLC
    张良雨, 于海英, 李思龙, 蒋亚奇, 王维剑, 李启艳, 李俊婕
    2024 (11):  1397-1404.  doi: 10.3969/j.issn.2097-2806.2024.11.015
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    采用国家药品监督管理局2021年第17号通告修订的“化妆品中防腐剂检验方法”项下的高效液相色谱法,对样品前处理条件进行了优化,并对市售随机抽取的160批牙膏中33种防腐剂进行了检测,将检验结果进行多维度统计分析,以了解防腐剂在此类产品中的使用情况及标签标识的准确性和真实性,为牙膏中防腐剂的安全评估与科学监管积累数据。结果表明,优化后的前处理条件可适用于牙膏中防腐剂的提取,且回收率良好。产自广东省和江苏省的样品最多,占总样本的54%。160批牙膏中共检出13种防腐剂,其中苯甲酸及其钠盐检出率最高,达35.0%;不同的牙膏产品中防腐剂添加量相差较大,检出量值最高的为苯甲酸及其钠盐,含量为0.44%,检出量值最低的为4-羟基苯甲酸及其钠盐,含量仅为0.00057%;38.1%的样品只检出1种防腐剂,34.4%的样品检出2种及以上防腐剂;61批牙膏检验结果与标签标识不符,其中以检出标签未标识的防腐剂情况居多,存在一定的安全风险。建议在今后的工作中持续关注牙膏的质量安全状况,加强牙膏产品的监管力度,督促企业规范化生产。

    Based on high performance liquid chromatography (HPLC) method under the item “Test Methods for Preservatives in Cosmetics” revised by the National Medical Products Administration Announcement No. 17 of 2021, the sample pretreatment condition was optimized, and 33 preservatives in 160 batches of toothpastes randomly selected from the market were tested. The detection results were subjected to multidimensional statistical analysis to understand the using status of preservatives in toothpastes, as well as the accuracy and authenticity of the labels, in order to accumulate data for the safety assessment and scientific supervision of preservatives in toothpastes. The results show that the optimized pretreatment condition is suitable for the extraction of preservatives from toothpastes, and the recovery rate is good. The samples produced from Guangdong and Jiangsu provinces are the most, accounting for 54% of the total samples. A total of 13 preservatives are detected in 160 batches of toothpastes, among which benzoic acid and its sodium salt has the highest detection rate, reaching 35.0%. The amount of preservatives added in different toothpastes varies greatly. The highest detected value is benzoic acid and its sodium salt, with a content of 0.44%; and the lowest detected value is 4-hydroxybenzoic acid and its sodium salt, with a content of only 0.000 57%. One type of the preservatives is detected in 38.1% of the samples, while two or more types of preservatives are detected in 34.4% of the samples. The detection results of 61 batches of the toothpastes are inconsistent with the labels, among which the majority of the preservatives not indicated on labels are detected, that will pose certain safety risks. It is recommended to pay continuous attention to the quality and safety of toothpastes in the future work, strengthen the supervision of toothpastes, and urge enterprises to standardize production.

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