基于光损伤皮肤成纤维细胞模型的鱼胶原蛋白肽修复性能研究

doi:10.3969/j.issn.2097-2806.2024.11.012

摘要/Abstract

摘要：

鱼胶原蛋白肽（Fish collagen peptides，FCPs）是一类具备多种活性功能的多肽混合物。近几年研究表明，口服鱼胶原蛋白肽能够安全高效地作用于皮肤，发挥抗氧化、抑制黑色素形成、延缓衰老和抗光老化等功效。然而，现有鱼胶原蛋白肽的体外功效研究较少。该研究利用人皮肤成纤维细胞建立UVA诱导的光损伤模型，从细胞形态和细胞存活率两个方面研究鱼胶原蛋白肽对人皮肤成纤维细胞增殖能力的影响。通过测定鱼胶原蛋白肽处理细胞后，其中透明质酸、Ⅰ型胶原、弹性蛋白含量以及总抗氧化能力的变化来研究其抗氧化功效。另外对比分析细胞形态、细胞活力和关键代谢产物等的具体变化来研究鱼胶原蛋白肽对UVA诱导的光损伤细胞的修复作用。该研究为鱼胶原蛋白肽在皮肤光损伤修复领域提供了理论依据，为新型功能食品和胶原蛋白产品的开发与应用奠定了基础。

关键词: 鱼胶原蛋白肽, 光损伤, 人皮肤成纤维细胞, 修复, 抗氧化

Abstract:

Fish collagen peptides （FCPs） represent a class of polypeptide mixtures endowed with diverse bioactive functions. Recent studies indicate that orally administered fish collagen peptides can effectively and safely impact the skin, exhibiting benefits such as antioxidation, inhibition of melanin formation, delayed aging, and resistance to photoaging. However, research comprehensively evaluating the efficacy of fish collagen peptides at the cellular level is relatively scarce. This study established a UVA-induced photodamaged human skin fibroblast model to investigate the impact of collagen peptides on the proliferation of human skin fibroblasts from both cellular morphology and viability perspectives. Additionally, alterations in hyaluronic acid, type Ⅰ collagen, elastin content, and total antioxidant capacity were assessed after treating cells with different samples. Through a comprehensive examination encompassing morphology, cell viability, and crucial metabolic substances, the photodamage repair effect of fish collagen peptides were comprehensively evaluated. This research provides experimental data support for oral collagen peptides in the field of skin photodamage repair, laying the groundwork for the development and application of novel functional foods and collagen products in the future.

Key words: fish collagen peptides （FCPs）, photodamage, human skin fibroblast, repair, antioxidation

中图分类号:

TQ658

蒲春宏, 王荣昌, 华中杰, 张佳婵, 陈朝. 基于光损伤皮肤成纤维细胞模型的鱼胶原蛋白肽修复性能研究[J]. 日用化学工业（中英文）, 2024, 54(11): 1375-1381.

Chunhong Pu, Rongchang Wang, Zhongjie Hua, Jiachan Zhang, Chao Chen. Study on the repair performance of fish collagen peptides based on the UVA induced fibroblast photodamage model[J]. China Surfactant Detergent & Cosmetics, 2024, 54(11): 1375-1381.

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项目名称（单位）	检测结果
灰分（g/100 g样品）	0.29
水分（g/100 g样品）	5.49
蛋白质（以干基计，g/100 g样品）	99.40

蛋白肽分子量范围/Da	峰面积百分比/ % （λ=220 nm）	数均分子量（Mn）	重均分子量（Mv）
>10 000	未检出	未检出	未检出
10 000~5 000	1.1	5 882	5 998
5 000~3 000	6.44	3 598	3 666
3 000~2 000	7.99	2 439	2 471
2 000~1 000	20.75	1 433	1 469
1 000~500	32.82	718	735
500~189	26.21	248	258
<189	4.69	—	—