甘草酸二钾通过调节巨噬细胞极化促进皮肤修复

doi:10.3969/j.issn.2097-2806.2024.11.009

摘要/Abstract

摘要：

为研究甘草酸二钾促进皮肤修复的作用机理及其与炎症反应的关系，用不同质量分数的甘草酸二钾处理LPS诱导的小鼠单核巨噬细胞（RAW264.7）炎症模型，通过流式细胞术、免疫荧光和ELISA的方法研究不同关键指标的变化。通过用甘草酸二钾和甘草酸二钾处理的RAW264.7细胞的培养液作为刺激物，处理人永生化角质形成细胞（HaCaT）细胞划痕模型，研究甘草酸二钾对皮肤修复作用的机制。结果显示，经不同质量分数的甘草酸二钾处理的RAW264.7细胞炎症模型，细胞膜上TLR2的表达降低，CD163的表达增加；RAW264.7细胞内的ROS水平降低；上清液中的NO，TNF-α和IL-6的含量显著降低，IL-4和IL-10的含量显著升高；用0.125%（w/%）甘草酸二钾处理的RAW264.7细胞的培养液作用于HaCaT细胞24 h，观察到HaCaT细胞迁移能力显著增强，然而甘草酸二钾直接作用于HaCaT细胞不能显著促进细胞迁移能力。综上，甘草酸二钾能够通过改善LPS诱导的RAW264.7细胞M1极化，调节炎症因子的分泌，达到促进HaCaT细胞迁移、促进皮肤修复的作用。

关键词: 甘草酸二钾, 巨噬细胞极化, 皮肤修复

Abstract:

The research aimed to study the mechanism of dipotassium glycyrrhizinate in promoting skin repair and its relationship with inflammatory reaction. The inflammatory model of mouse monocyte macrophage （RAW264.7） induced by LPS was treated with different mass fractions of dipotassium glycyrrhizinate, and the expressions of TLR2 and CD163 on the surface of RAW264.7 cells were detected by flow cytometry. The results show that dipotassium glycyrrhizinate inhibits the expression of TLR2 on the cell membrane and promotes the expression of CD163, indicating that dipotassium glycyrrhizinate inhibits the M1 polarization of macrophages and promotes the M2 polarization. It is confirmed by immunofluorescence that dipotassium glycyrrhizinate can prevent M1 polarization by inhibiting the production of ROS in macrophages. By detecting the NO content and the secretion of TNF-α, IL-4, IL-6 and IL-10, the inflammatory factors and mediators related to M1/M2 polarization were studied. Among them, the contents of NO, TNF-α and IL-6 decrease, while the contents of IL-4 and IL-10 increase, which prove that dipotassium glycyrrhizinate regulates LPS-induced M1 polarization of macrophages and promotes the secretion of M2 polarization marker protein. In order to study the mechanism of promoting skin repair by dipotassium glycyrrhizinate, the scratch and proliferation experiments of HaCaT human keratinocytes （HaCaT） were carried out with different mass fractions of dipotassium glycyrrhizinate and the culture medium of RAW264.7 cells treated with dipotassium glycyrrhizinate. The results confirm that dipotassium glycyrrhizinate can promote the proliferation of HaCaT cells, but can not directly promote the migration of HaCaT cells. However, it can promote the migration and proliferation of HaCaT cells by regulating the polarization of RAW264.7 cells. To sum up, dipotassium glycyrrhizinate can improve the M1 polarization of RAW264.7 cells induced by LPS, regulate the secretion of inflammatory factors, and promote the migration of HaCaT cells and skin repair.

Key words: dipotassium glycyrrhizinate, macrophage polarization, skin repair

中图分类号:

TQ658

王学文, 刘慧, 王倩, 杨素珍, 刘菲. 甘草酸二钾通过调节巨噬细胞极化促进皮肤修复[J]. 日用化学工业（中英文）, 2024, 54(11): 1355-1361.

Xuewen Wang, Hui Liu, Qian Wang, Suzhen Yang, Fei Liu. Dipotassium glycyrrhizinate promotes skin repair by regulating macrophage polarization[J]. China Surfactant Detergent & Cosmetics, 2024, 54(11): 1355-1361.

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分组	迁移率/%	SD值	P值（与NC组比较）
BC	69.10	3.70	0.009 0^**
NC	57.41	1.60
0.125%甘草酸二钾	63.79	1.32	0.019 8^*
0.062 5%甘草酸二钾	58.71	0.40	0.338 0
0.031 25%甘草酸二钾	55.55	0.99	0.263 6

分组（w/%）	迁移率/%	SD值	P值（与0%组比较）
0	36.67	3.30
0.125	43.14	0.87	0.056 3
0.062 5	39.13	0.65	0.360 5
0.031 25	36.06	1.65	0.822 1