关键词: 乳酸, 复方精油, 微乳, 响应面法, 脂溢性脱发

Abstract:

This study utilizes a lactic acid solution, which promotes hair growth, as the aqueous phase, and compound essential oil as the oil phase, to prepare an O/W microemulsion for preventing hair loss and promoting hair growth. The formulation of the microemulsion was optimized using a pseudo-ternary phase diagram combined with the Box-Behnken response surface method, evaluating particle size and PDI as indicators to obtain the best preparation process. A seborrheic alopecia animal model was established using C57BL/6 mice to evaluate the effects of the microemulsion on hair loss prevention and hair growth. The results indicate that the optimal formulation of the microemulsion comprise 9.29% oil phase, 21.32% Tween 80, 10.66% anhydrous ethanol, and 58.71% aqueous phase, yielding an average particle size of 16.48 nm and a PDI of 0.038. The microemulsion exhibits good centrifugation and temperature stability. The formulation significantly increases hair length and weight in mice （P<0.05）, improves the number and distribution of hair follicles, upregulates the concentrations of KGF and VEGF in mouse skin, and decreases the concentration of TGF-β1 （P<0.05）. This study provides a basis for the development of new formulations containing essential oils for hair loss prevention and promotion.

Key words: lactic acid, essential oil of traditional Chinese medicine, microemulsion, Box-Behnken, seborrheic alopecia